低温保存

C组：组织工程骨未行低温保存；

Group C : Tissue engineered bone without cryopreservation .

拟南芥幼苗超低温保存后DNA甲基化的遗传变异

Variation of DNA Methylation in Arabidopsis thaliana Seedlings After the Cryopreservation

观察深低温保存不同时期人视网膜的酶组织化学及超微结构变化

To observe the enzymic histochemical and ultrastructural changes of cryopreserved human retina .

男孩的伤口包扎好，断肢低温保存后，被送往和田机场赶往乌鲁木齐的航班。南航和田营业处和新疆和田机场塔台迅速将此情况反馈至上级部门。

After the boy 's wounds were bound up and the severed limb was preserved at a low temperature , he was sent to the Hotan airport to catch a flight to Urumqi .

目的探索4°C低温保存肝细胞的方法。

Objective To explore an optimal method for hypothermic preservation of hepatocytes at 4 ° C.

线粒体ATP敏感性钾通道在心脏低温保存中的作用研究

Effect of Mitochondrial ATP-Sensitive Potassium Channel on Hypothermic Preserved Rat Myocardium

大剂量~（60）Coγ照射加深低温保存对大鼠同种带瓣主动脉活性的影响

Endothelial cell viability in rat aortic valve homograft after high-dose ~ ( 60 ) Co γ irradiation and cryopreservation

低温保存-再灌注肝脏肝细胞凋亡与肝细胞糖原含量的关系及bax基因在其中的作用

Relationship Between Hepatocellular Apoptosis and Glycogen Contents During Hepatic Cold Preservation Reperfusion and Its Mechanism

最后剔除断裂及降解的、纯度较低的DNA样本，超低温保存，从而建立了中国少数民族基因组DNA样本库。

At last , we removed the DNA samples of breakage and degradation or lower purity , thereby establishing a genomic DNA library of Chinese ethnic minorities .

结论外源性ATP在大鼠肝脏低温保存中有保护作用，但需Mg2+参与；

Conclusions Exogenous ATP showed protective effects on rat liver during cold storage , Mg 2 + was indispensable .

伴随IVF-ET等人类助孕技术的发展，低温保存技术起到了不可估量的作用。

Following the development of IVF-ET , cryopreservation has an inestimable effects .

神经干细胞（NeuralStemCells）的移植对于治疗各种神经退行性疾病和组织缺陷性疾病有广阔的临床应用前景，其低温保存对于发挥其组织修复和医疗效力有至关重要的作用。

The transplant of neural stem cells has excellent future for healing some neurodegenerative diseases and tissue defects . The cryopreservation of NSCs play significant role in tissue repair and medical treating .

芸芥（ErucasativaMill）发状根的诱导、超低温保存及次生代谢物的分析

Induction and Cryopreservation of Eruca Sativa Mill Hairy Roots and Analysis of Its Secondary Metabolites

结论肝脏低温保存再灌注过程中，肝细胞内糖原能明显拮抗肝实质细胞凋亡的发生，其内在机理可能为肝细胞糖原通过抑制bax基因蛋白的表达从而达到拮抗肝实质细胞凋亡的发生。

Conclusion Intracellular abundant glycogen may significantly depress the hepatocellular apoptosis during hepatic cold preservation reperfusion by decreasing hepatocellular bax gene expression .

Euro－Collins液加前列腺素E1与低钾右旋糖酐液低温保存供体兔肺18h的实验研究

Study on the Effects of Modified Euro Collins Solution With Prostaglandin E 1 and Low Potassium Dextran Solution on Rabbit Lung Preservation

使用超滤管进行浓缩除盐，最后使用Bio-Radproteinassayreagent进行蛋白定量，低温保存备用。

Utilize 5K hyperfiltration tube to condense and demineralization . Finally , quantitate the proteins with Bio-Rad protein assay reagent , Cryopreservation standby . 2 .

目的探讨改良高渗枸橼酸盐嘌呤肾保存液（HCA）对离体肾脏的低温保存效果。

Objective To evaluate the protecting effects of Modified HCA Preservation Solution on Isolated Canine kidneys .

结果与C组比较，H组供心低温保存后含水率、MDA高于C组，而ATP明显降低，差异均有显著性（P<0.05）；

Results Compare with group C , heart tissue water content and MDA content were increased ( P < 0.01 ) and ATP decreased ( P < 0.01 ) in group H after storage for 4 h.

目的：探讨大鼠肝脏低温保存及常温缺血再灌注过程中，不同保存液中嘌呤核苷磷酸酶（PNP）活性的变化。

Objective : To study the change of purine nucleoside phosphorylase ( PNP ) during ischemia and reperfusion in rat liver .

低温保存（hypothermicpreservation）是目前保持肝细胞生物学活性的重要方法，可分为两大类：一类为4℃左右短期保存；

Hypothermic preservation , an important means to keep the biological activities of hepatocytes , can be mainly divided into two kinds : one is short storage at about 4 ℃;

采用不同的拟南芥ABA突变体，超低温保存后研究其存活率的不同，进而探讨ABA信号转导途径与低温胁迫，脱水胁迫信号转导途径间的相互作用。

Cells of different ABA synthesis or response mutants of Arabidopsis thaliana had different viability after cryopreservation , which revealed ABA has a closed relationship with cold - and dehydration-stress signaling transduction .

血小板胞内冰晶形成（IIF）的温度是指导血小板低温保存最重要的物理参数之一。

The temperature of platelet intracellular ice crystal formation ( IIF ) is one of the most important physical parameters to instruct platelet cryopreservation .

果树休眠枝的低温保存对T-N和T-P的去除效果较差。

Preservation For Temperate Fruit Dormant Shoots In Low Temperature and for T-N and T-P are quite low .

用终浓度4%二甲基亚砜（DMSO）作为防冻剂，于30℃和80℃冷冻保存血小板以开展低温保存血小板的输注。

Using the dimethylsulfoxide ( DMSO ) with final concentration of4 % as cryoproteclant , the human platelets were stored at-30 ℃ and-80 ℃ to provide cryopreserved platelets transfusion service .

采用玻璃化法成功地超低温保存了胡萝卜悬浮培养细胞及其原生质体，存活率分别为83.3%（TTC法检测）和47%（FDA法检测）。

The survival rates for carrot cells and protoplasts cryopreserved by vitrification were 83.3 % ( based on the TTC reduction ) and 47 % ( based on the FDA assay ) respectively .

方法采用细胞培养技术和流式细胞仪技术研究液氮低温保存对大鼠主动脉平滑肌细胞和成纤维细胞主要组织相容性抗原类（MHC类抗原）表达的影响。

METHODS By means of cell culture and flow cytometry we studied the effect of fluid nitrogen cryopreservation on the expression of MHC ⅰ antigen of smooth muscle cells and fibroblasts of aortic homografts of rats .

目的研究三七总皂甙（PanaxNotoginsengSaponins，PNGS）对大鼠肝脏低温保存再灌注的影响及其机制，并筛选三七总皂甙保存肝脏的适宜浓度。

Objective To investigate the effects and mechanism of Panax Notoginseng Saponins ( PN6S ) on the rat liver during cold preservation , and screen optimal concentration of PNGS on the rat liver .

主要阐述了在以二甲亚砜为抗冻剂，不同的4℃平衡时间对PCK细胞低温保存的影响。

This paper mainly described the effect of the different equilibrium time on cryopreservation of PCK cells when dimethylsulphoxide ( DMSO ) was used as the cryoprotectant .

结论：XJ超极化心脏保存液在低温保存供心4～6h心肌超微结构变化不明显，心脏保存10h后心肌超微结构改变较8h明显。

Conclusion Hypothermia preservation of donor heart by X-J hyperpolarized cardiac preservation solution for 4 ~ 6 hours causes no significant changes on ultrastructure , while 10 hours preservation causes significant change compared with 8 hours preservation .

近年来，AQPs在细胞转运水和冷冻保护剂中的作用倍受关注，AQPs能提高某些细胞和组织的冻融存活率，在低温保存中具有一定的应用前景。

Recently the roles of AQPs in the transport of water and cryopreservation are attracting considerable interest as they can improve the survival of cells and tissues during freezing / thawing .