伊蚊

三种伊蚊细胞系基因组多态性DNA的研究

Study on the Genomic Polymorphic DNA of Three Species of Aedes

中华按蚊和白纹伊蚊涎腺及血淋巴中蛋白质的PAGE分析

The Protein of Salivary Gland and Hemolymph of Anopheles sinensis and Aedes albopictus in PAGE

中国不同地理株白纹伊蚊细胞色素C氧化酶亚基Ⅰ基因序列比较

Sequences analysis of cytochrome C oxidase subunit ⅰ gene in Aedes albopictus from different geographic strains in China

结果各地理株白纹伊蚊COⅠ基因片段序列长度均为415bp，所测各株序列均无缺失。

Results Sequence length of CO ⅰ fragment in each geographic strains was 415 bp .

溴氰菊酯抗性白纹伊蚊抑制消减cDNA文库的构建

Construction of Suppression Subtracted cDNA Library of Deltamethrin-resistant Aedes albopictus

目的：1.分析广州市内不同群体白纹伊蚊细胞色素C氧化酶亚基I基因遗传多态性。

To analyse the genetic polymorphism of mitochondrial cytochrome C oxidase subunit I gene in different populations of Aedes albopictus from Guangzhou . 2 .

白纹伊蚊Rh类糖蛋白基因的克隆与生物信息学分析

Cloning and Bioinformatics Analysis of A Rhesus-like Glycoprotein from Aedes albopictus

目的从白纹伊蚊基因组DNA中获得乙酰胆碱酯酶（AchE）基因片段及其DNA序列。

Objective To get the clone of the acetylcholinesterase ( AchE ) fragment and its DNA sequence from Aedes albopictus .

2种杀虫剂对白纹伊蚊幼虫的孳生地具有良好的控制作用，经趋势检验，两种杀虫剂的P值均小于001，表明幼虫孳生阳性率随时间逐渐降低，效果显著。

The trial also showed both insecticides had good effects on larvae in breeding-ground , and the positive rate of larvae in breeding-ground gradually reduced as the time went on .

抗登革病毒E蛋白单克隆抗体的制备及鉴定登革病毒感染对白纹伊蚊生理生态特性的影响

Preparation and Characterization of Monoclonal Antibody Against DV Envelope Pretein ; The Influence of Dengue Virus Infection Upon the Physiological and Ecological Natures of Aedes albopictus

1316只蚊分为116组，经C6／36白纹伊蚊细胞培养和单克隆抗体间接免疫荧光试验检测，全部未分离出DF病毒。

1316 mosquitoes divided into 116 groups were tested and no dengue virus was isolated .

不同发育期中华按蚊、白纹伊蚊LDH和EST同工酶的比较研究

Comparative Studies on LDH and EST Isoenzymes in Larvae , Pupae and Adults of Anopheles sinensis and Aedes albopictus

采用生物化学方法对α-T作用后白纹伊蚊幼虫蛋白质含量、酯酶活力及MDA（丙二醛）进行测定。

To detect protein content , esterase activity and MDA content of Aedes albopictus 4th larvae by biochemistry method after treated by UVA and alpha-terthienyl .

深圳市白纹伊蚊rDNAITS2区克隆及SNP分析

Cloning and SNP analysis of rDNA ITS 2 of Aedes albopictus in Shenzhen

白纹伊蚊感染登革2型病毒24h后，和对照蚊虫一起提取RNA，用差异显示PCR（DDRTPCR）技术对蚊虫感染病毒后基因表达的变化进行研究。

Differential expressed genes of Aedes albopictus was studied by DDRT-PCR technique , 24h after mosquito infected with dengue type 2 virus .

埃及伊蚊抗凝血因子Xa基因片段的克隆测序

Part Gene Sequence of Factor Xa-directed Anticoagulant from the Vector Mosquito Aedes aegypti in China

方法：用兼并引物PCR方法从我国的埃及伊蚊基因组DNA扩增抗凝血因子Xa基因片段，克隆入测序T载体进行测序。

Methods : Degenerate oligo-nucleotide primers PCR was used to amplify from its genome DNA , then the amplified fragment was cloned into T vector to sequence .

核酸杂交和McAb－IFA快速检出鉴定白纹伊蚊体内登革热病毒的研究

Use of McAB ─ IFA and Nucleic Acid Hybridization for Detecting Dengue Virus in Infected Aedes Aegypti Mosquitoes

观察白纹伊蚊幼虫吸食α-T并受UVA照射后生长发育的改变。

To determine the effect of alpha-terthienyl on the growth of Aedes albopictus larvae under UVA .

白纹伊蚊和埃及伊蚊防御素（defensin）成熟肽基因克隆及结构推测

Gene cloning of mosquito defensin mature peptide and conjecture of the secondary and tertiary structure

2种杀虫剂喷洒处理24h后，笼内白纹伊蚊校正死亡率均大于99%，说明白纹伊蚊对2种杀虫剂都非常敏感。

More than 99 % calibrated mortality of Aedes albopictus in cages were observed for 24 hours after the trials of both insecticides .

2002年，支国舟等在中国首先克隆了埃及伊蚊和白纹伊蚊（Aedesalbopictus）的防御素基因并进行序列分析。

Cloned and analysed the defensin gene of Aedes aegypti , and Aedes albopictus at first in 2002 .

方法根据已发表的埃及伊蚊和冈比亚按蚊防御素基因序列设计合成两对引物，以中华按蚊成蚊cDNA为模板进行PCR扩增，将预期片段克隆测序并进行分析；

Methods Two pairs of primer were designed and synthesized based on the published defensin sequences , and PCR amplification was performed using the cDNA of An . sinensis as the template .

白纹伊蚊乙酰胆碱酯酶基因片段简并引物PCR、克隆及鉴定随机引物聚合酶链反应法鉴定2例无菌性脑炎的未知病毒

Degenerate Primers Polymerase Chain Reaction Clone and Identification of the Acetylcholinesterase Gene Fragment from the Mosquito , Aedes albopictus ; Detection of Unknown Virus from 2 Case of Aseptic Meningitis Epidemic Using Random Primer PCR Assay

【目的】PCR法扩增与测定白纹伊蚊和致倦库蚊的细胞内共生菌Wolbachia16SRRNA序列，分析其种系发生关系及传播方式。

Objective To amplify and analyze the Wolbachia 16S rRNA sequences from Aedes albopictus and Culex pipiens quinquefasciatus , and to analyze their phylogenetic relationship . I is presented . relation .

在近紫外光（UVA）下，确定黑暗中不同处理时间与α-三噻吩（α-T）对白纹伊蚊幼虫毒杀作用的关系。

To identify the relationship between different treating time in dark and the toxicity of alpha-terthienyl under UVA to Aedes albopictus larvae .

蚊虫的防御素基因首先从埃及伊蚊中克隆，2000年Eggleston等报道了冈比亚按蚊防御素基因的DNA结构及其免疫调节作用。

The defender genes of mosquitoes firstly clone from aedes . Eggleston ( 2000 ) reports DNA structure of the defender genes of anopheles and its immune regulation .

目的测定和比较中华按蚊、白纹伊蚊和致倦库蚊细胞色素C氧化酶亚基II（COII）基因全序列，分析3种蚊虫之间及与其它几种蚊虫的COII基因序列的同源性。

Objective To determine and compare the complete sequence of COII gene from Anopheles sinensis , Aedes albopictus and Culex quinquefasciatus and to identify the molecular homology among these mosquitoes .

方法DV2经胸注射入白纹伊蚊。

Methods DV2 were injected into the thorax of Ae .

光活化杀虫剂K-01对白纹伊蚊幼虫的毒杀作用

Toxicity of photoactivated insecticide K-01 to the larvae of Aedes albopictus