人胰岛素

重组人胰岛素及C肽的分离纯化

Separation and purification of recombinant human insulin and C peptide expressed in the yeast Pichia pastoris

B（19）-Gly-B（20）人胰岛素的分离纯化及性质研究

Research on the purification and characterization of B_ ( 19 ) - Gly-B_ ( 20 ) human insulin

人胰岛素原C肽的合成及专一性抗体制备

Synthesis of Human Proinsulin C-peptide and Preparation of Specific Antibody to It

人胰岛素原C肽的表达、纯化及其细胞活性研究

Expression and Purification of Proinsulin C-peptide and its Cell Bioactivity

人胰岛素原C肽固相合成的改进

An Improved Solid Phase Synthesis of Human Proinsulin C-peptide

人胰岛素原C肽单克隆抗体的研究

Studies on Monoclonal Antibodies Directed Against Human C-peptide

在非p细胞内不能合成人胰岛素。

Human proinsulin is not effectively coverted to mature insulin in non - β - cells .

Met-Lys-双C肽人胰岛素原基因的构建表达及分离纯化

Construction of Met Lys Double C Peptide Human Proinsulin Gene and Its Expression and Characterization

hGH-双精氨酸C肽人胰岛素原基因的克隆表达及纯化研究

Study on Cloning , Expression and Purification of hGH-double Arg C Peptide Human Proinsulin Gene

人胰岛素原DNA疫苗预防非肥胖糖尿病鼠胰岛β细胞凋亡的机制探讨

Mechanisms of human proinsulin DNA vaccine preventing islet beta-cell apoptosis in NOD mice

目的：构建一种快速、高效的人胰岛素原C肽基因的表达载体，为有效地提高重组C肽的产量奠定了基础。

Objective : A rapid strategy for high-level expression vector expressing human c-peptide gene was described .

人胰岛素原DNA疫苗的构建与鉴定

Construction and Identification of Human Proinsulin DNA Vaccine

表达的融合蛋白质通过设计的特异性位点的酶切得到重组的人胰岛素原C肽单体。

Coli and the recombinant proinsulin C peptide was produced through site specific cleavage of the resulting gene products .

首先，构建克隆载体：将改造的人胰岛素样生长因子（huIGF-I）基因直接克隆到T-载体，并进行DNA序列测定；

First , cloning vector wais constructed : The gene encoding human insulin-like growth factor-I was modified .

人胰岛素A链与B链基因的化学合成、克隆与表达

Chemical synthesis , cloning and expression of human insulin A chain and B chain gene separately in E. coli

目的：探讨人胰岛素C肽对（？）糖尿病大鼠动脉粥样硬化相关因子的影响。

OBJECTIVE : To investigate the correlativity between the C-peptide in different concentration and the relative factors of atherosclerosis in diabetes rats .

同时，也用PAM树脂合成了人胰岛素原C肽类似物。

The preparation human proinsulin C-peptide of analogue using of PAM resin was also reported .

重组人胰岛素类似物的HPLC分析

The Analysis of Insulin Lispro by HPLC

突变的人胰岛素原CDNA测序结果见后。

Measure sequence confirms that sequence of modified human proinsulin cDNA is correct .

方法1RT-PCR方法从胎儿胰腺获取野生型人胰岛素原cDNA。

Methods Native human insulin cDNA was derived from fetus pancreas by RT-PCR .

简捷的PCR点突变实现人胰岛素原基因非β细胞表达

Expression of human proinsulin gene in non - β - cells by point mutation with concise PCR

U基因。人胰岛素原基因突变体的构建

Construction of a mutant human proinsulin gene

GC盒不是人胰岛素基因启动子中关键顺式作用元件

GC - box is not a key cis - acting element in human insulin gene promoter

结论用重组人胰岛素诺和灵N联合口服降糖药治疗老年糖尿病是安全有效的。

[ Conclusion ] Recombinant human insulin injection Novolin N and oral hypoglycemia agent combination therapy on elderly diabetes mellitus is safety and effective .

用RP-HPLC法对基因工程人胰岛素纯度的评价

Evaluation of purity for recombinant human insulin by RP-HPLC

目的探讨人胰岛素原DNA疫苗预防非肥胖糖尿病（NOD）小鼠胰岛β细胞凋亡的机制。

Objective To investigate the mechanisms of human proinsulin DNA vaccine preventing islet β - cell apoptosis in NOD mice .

RP-HPLC法测定鼻用重组人胰岛素粉雾剂中的相关蛋白质

Determination of Relevant Protein in Recombinant Human Insulin Intranasal Dry Powder Inhalation by RP-HPLC

目的：分析深圳市机关职员1146人胰岛素抵抗（InsulinResistance，IR）与血脂变化的关系。

AIM : To analyze the relationship of insulin resistance ( IR ) with the changes of blood lipid in 1 146 officials of Shenzhen city .

猪胰岛素与人胰岛素的差别仅为B链30位一个氨基酸不同，猪胰岛素为丙氨酸，人胰岛素为苏氨酸。

The only difference between porcine insulin and human insulin is the 30 ~ th amino acid of B-chain , porcine insulin is alanine , and the latter is threonine .

定点突变的人胰岛素基因在CHO细胞表达的研究

A study on the expression of site-directed mutated human insulin gene in CHO cell