胚体

悬浮培养ES细胞能得到囊状胚体。

Culturing single suspension ES-like cell formed cystic embryoid body .

胚体培养对小鼠ES细胞定向神经分化的影响研究

The Effects of Embryonic Bodies on Differentiation of Mouse Embryonic Stem Cells to Neuronal Cells in Vitro

方法：胚胎干细胞在无饲养细胞、无碱性成纤维细胞生长因子（bFGF）、悬浮条件下培养形成类胚体。

Meth-ods : Human embryonic stem cells were cultured in suspension without feeder layers and bFGF in medi-um .

方法：首先培养用于迁移实验的细胞，即源于类胚体的nestin阳性前体细胞。

Methods : The first step was to prepare nestin-positive precursor cells for cell migration experiments .

一种利用STO饲养层细胞制备拟胚体的新方法

A Novel Method to form Embryoid Bodies Using STO Cells as Feeder Layers

目的探讨类胚体中残留未分化胚胎干细胞（ESC）的特性。

Objective To explore the residual undifferentiated mouse embryonic stem cells ( ESCs ) in embryoid bodies .

EGFP标记胚胎干细胞体外向心肌样细胞分化及拟胚体形成的初步研究

Labeling Embryonic Stem Cell with EGFP Differentiation into Cardiomyocyte-Like Cells in Vitro and the Preliminary Study of Embryoid Body Formation

采用类胚体法（EB）的EB分化4-/4+程序和单层分化法研究不同的实验方法对胚胎干细胞神经分化的影响。

The neural differentiation process of mouse embryonic stem ( ES ) cell induced by embryoid differentiation and monolayer differentiation was investigated in this study .

采用碱性磷酸酶染色、核型分析、类胚体实验对扩增细胞进行ESC鉴定。

Subsequently we identified the expanding cells by alkaline phosphatase staining , karyotype analysis and the formation of embryoid body which are typical characteristics of ESCs .

就提高小鼠免疫能力而言，以10d龄的鸡胚尿囊液和胚体悬液，剂量为0.4mL/（只·日）效果最好.有关鸡胚尿囊液在功效学方面的作用，有待进一步研究。

For promotion of immunity , 10d chick allantoic fluid and suspension with dose of 0.4 mL / 1 brings in top effect .

ESCs体外诱导为神经细胞的经典途径是先通过拟胚体阶段以启动分化，再通过RA或无血清培养法诱导拟胚体分化为神经细胞。

The typical method of ES cells differentiation into neural cells in vitro includes embryoid bodies ( EBs ) formation and subsequent differentiation into neural cells under all-trans retinoic acid or serum-free conditions .

随着鸡胚内、外血管的发育，PGCs则进入血管中，并顺着血管于孵化约36h（10期）时首次迁移到胚体上。

Later , these PGCs entered the blood vascular system and appeared first in the embryo proper at stage 10 ( about 36 h post incubation ) .

方法悬浮法培养类胚体14d，10%中性甲醛固定，常规石蜡包埋切片，取具有子宫内膜样组织结构的切片行雌激素受体（ER）免疫组织化学染色。

Methods ( 1 ) Embryoid bodies were cultured in suspension for 14 days , fixed in 10 % neutral-buffered formalin and embedded in paraffin . Estrogen receptor ( ER ) was detected by immunocytochemical staining .

ES细胞在体外分化所形成的拟胚体在结构上能够模仿早期胚胎发育过程，包括在内细胞团表面形成内胚层、柱状上皮细胞的分化，以及中央空腔的形成。

When cultured as aggregates embryoid bodies derived from ES cells recapitulate early steps of embryo development in structure including formation of endoderm on the surface of the inner cell mass differentiation of columnar epithelium and formation of central cavity .

在三维的悬浮拟胚体培养体系，ECs的分化不依赖于外源性生长因子，分化表现为条索状结构、管腔样结构及排列紊乱的细胞团三种形式。

In the suspended embryoid bodies ( EBs ) culture system of three-dimensional differentiation , three structures were found without addition of exogenous growth factors : cord-like structure , vessel-like structure and disordered cell mass .

目的：探讨不经拟胚体（EB）培养阶段，应用维甲酸（RA）在体外直接将胚胎干细胞（ESC）诱导分化为神经细胞的可行性和效果。

AIM : To investigate the feasibility and effect of directly differentiation of embryonic stem cells ( ESC ) into neural cells induced by retinoid acid ( RA ) without embryonic body ( EB ) culture period in vitro .

将小鼠5d龄ES-D3细胞源的类胚体（EBs）单细胞，按5×104/mL接种6孔细胞培养板，在DMEM基础培养基中使EBs单细胞贴壁培养。于第14！

The cells of 5-day embryoid bodies ( EBs ) derived from murine ES-D3 lineage were inoculated into each well of 6-well culture plate at 5 × 104 / mL in DMEM .

方法先诱导mESCs形成类胚体（EB），进一步诱导其分化为成纤维细胞（mEB-dFE），以用作饲养细胞。

Methods mESCs were induced to form mouse embryoid bodies ( EB ), and then fibroblast-like cells were derived from further differentiated mEB ( mEB-dF ), which served as feeder cells .

本研究发现：小鼠ES细胞在体外分化5～14d形成的拟胚体中含有HPPCFC。

In this study the HPP CFC could be detected within EBs differentiated for 5 to 14 days of murine ES cells , but the development dynamics of the HPP CFC differed greatly among distinct serum lots .

以上结果表明，诱导hAFS细胞向心肌细胞分化时，通过形成类胚体并采用条件培养液的诱导效果最好。

The conclusion showed that the induce effect of hAFS cells differentiation into cardiomyocytes through the formation of embryoid bodies under the conditional medium was advantageous over other induce conditions .

结果：10、30、50、70mg/ml丹参在拟胚体形成的第10、11、12d均可明显提高心肌细胞的分化率（P<0.01）。

Results : On days 10,11 , and 12 of EBs generation , 10,30,50 , and 70 mg / ml of salvia miltiorrhiza significantly increased the percentage of cardiomyocytes ( P < 0.01 ) .

水温18～26℃下，20-24h胚体发育到D形幼虫，6～14d幼虫开始变态，8&17d变态为稚贝。

At water temperature 18 - 26 ℃ , it takes 20 - 24 hours for the embryo to develop to veliger larvae , 6 - 14 days to begin metamorphosis , and 8 - 17 days to develop to naiad larvae .

方法：将ES-D3细胞形成4天拟胚体（4dEBs），再用骨髓内皮细胞条件培养液（mBMEC-CM）和/或胎肝基质细胞条件培养液（FLSC-CM）诱导4dEBs生成造血干细胞。

Methods ES-D3 cells by formation of the day-4 embryoid bodies ( 4dEBs ) were induced into hematopoietic stem cells by co-culture with murine bone marrow endothelial cell-conditional medium ( mBMEC-CM ) and the fetal liver stromal cell-conditional medium ( FLSC-CM ) .

绛色细胞由胚体1～8腹节的外胚层部分细胞发育而成。

Oenocytes derived from ectodermal cells of 1 to 8 abdomen .

铁皮石斛原胚体液体悬浮培养条件

Study on the condition of pro-embryoids suspension culture of Dendrobium candidum

竹果胚体类型及其系统分类

The embryonic types of the bamboo fruits and their systematic classification

胚体大小也显著增加。

Arboreum , and the embryo size also increased evidently .

胚胎期死亡时表现为胚体腐烂；

At the embryo stage , the embryo became putrid after death .

向日葵未成熟胚体细胞胚胎发生的研究

Studies of the somatic embryogenesis in young embryos of sunflower

利用小鼠胚胎干细胞贴壁制备拟胚体的新方法研究

A novel method for preparing embryoid bodies with attached embryonic stem cells