胚性愈伤组织

BA能促进愈伤组织的生长，但对胚性愈伤组织的诱导有抑制作用。

BA promoted callus growth but inhibited embryogenic callus induction .

胚胎发生：由胚轴诱导产生的淡绿色、生长迅速的愈伤组织在含NAA和2，4-D的培养基上培养4周后可形成黄白色疏松易碎的胚性愈伤组织。

Somatic embryogenesis : NAA and 2,4-D help callus form light yellow , frail embryogenic callus .

小麦胚性愈伤组织导入外源DNA的探讨

Experiments of introducing exogenous DNA to wheat embryo callus

玉米胚性愈伤组织经不同方法导入大豆DNA形成的幼苗同工酶分析

Analysis on the isozyme of maize 's seedling acquired through transmitting DNA of soybean to embryogenic calli with different methods

玉米叶片胚性愈伤组织诱导及其与内源IAA和ABA关系的初步研究

Embryogenic Callus Induction of Maize Leaves and Related to Endogenous IAA and ABA

ABA、NAA诱导水稻胚性愈伤组织的研究

Studies on the induction of embryogenic callus in Rice by aba , NAA

MB培养基是最适的胚性愈伤组织诱导培养基；

MB was the optimal medium for embryogenic development .

荷兰芹胚性愈伤组织诱导及胚状体发生与内源IAA和ABA关系的初步研究

Induction of embryonic callus and formation of embryoid related to Endogenous IAA and ABA in Celery

LM培养基上诱导产生极少量的胚性愈伤组织，在后期增殖过程中死亡。

LM medium induced a little embryonic callus and died during the proliferation .

渗透胁迫下芦苇胚性愈伤组织中脯氨酸含量的变化及外源ABA的影响

Changes of Free Proline Contents of the Calluses from Reed Ecotypes to the Osmotic Stress and Exogenous ABA

这些结论表明胚胎形成需要相对低的DNA胞嘧啶甲基化水平，而对于胚性愈伤组织的分化过程则需要高甲基化。

These results suggest that embryogenesis maybe need a relatively low level of cytosine DNA methylation , while for differentiation from embryogenic calli , a higher methylation level is necessary .

2,4-D是诱导胚性愈伤组织所必须的，NAA对愈伤组织的诱导效果弱于2,4-D。

It was found 2,4-D essential for embryogenic callus induction , but not NAA .

在胚性愈伤组织时期，RNA的含量很低，而DNA合成十分活跃。

At embryogenic callus stage RNA contents were very low , while DNA was actively synthesized , closely related to the active synthesis of proteins at this stage .

非胚性愈伤组织细胞的ATP酶定位于液泡，与液泡的水解功能有关。

In the cells of non-embryogenic calli , ATPase located in vacuoles and involved in the function of the hydrolysis of vacuole .

研究2,4-D和6-BA对诱导胚性愈伤组织的影响。

The experiment was made on the influence of 2,4-D and 6-BA on embryogenic callus induction .

克隆了龙眼胚性愈伤组织2个类型的磷酸丙糖异构酶基因（TPI）。

Two longan embryogenic callus triosephosphate isomerase ( TPI ) genes were cloned .

方法以高羊茅（Fes-tuca.arundinacea）成熟种子为外植体，以MS为基本培养基，进行高羊茅胚性愈伤组织诱导、继代、分化及生根培养。

Methods Use mature seeds of fescue arundinacea and MS medium were respectively as explants and basical medium to perfume embryonic callus induction , subculture , differentiation and rooting medium .

本试验通过控制2,4-D浓度与生长发育时间来进行龙眼胚性愈伤组织体胚发生同步化调控；

In this experiment , longan embryogenic callus was synchronized by controlling 2,4-D concentration and development time ;

POD同工酶的P3带是胚性愈伤组织特有的酶带，表明胚性存在。

Band P3 of POD isoenzyme was the specific band of embryogenic callus , and it might show the embryogenic capability .

在低浓度2,4-D和添加了ABA的继代培养基中，愈伤组织质量明显改善，胚性愈伤组织增加。

By reducing consistence of 2,4-D and adding ABA in subculture medium , the quality of embryogenic callus was improved .

结果表明，胚性愈伤组织开始分化后，DNA、RNA和可溶性蛋白质含量迅速增加，到胚状体形成时达到高峰。

The results showed that the contents of DNA , RNA and protein increased rapidly while the beginning of the embryogenic calli differentiation . The peak arrived at the formation of somatic embryo .

MSB培养基中添加IBA和BA也不能直接诱导获得胚性愈伤组织。

Embryogenic callus could be directly obtained on the MSB sold medium with the combination of IBA and KT .

以石刁柏嫩茎为材料，研究了2,4-D对其初始愈伤组织诱导和胚性愈伤组织发生的影响。

We studied effect of 2,4-D on initial callus inducing and embryogenic callus occurring from Asparagus tender stem culture .

研究了PEG-6000胁迫下两种生态型芦苇胚性愈伤组织游离脯氨酸含量的变化及外源ABA对其变化的影响。

The changes of free proline contents of the calluses from two kinds of reed ecotypes have been detected .

试验结果表明：培养基组合NN（NMB+NB）有利于胚性愈伤组织的诱导和分化。

The results showed that the best media NN ( NMB + NB ) were in favor of the induction and regeneration of embryonic callus .

龙眼松散型胚性愈伤组织阶段可溶性蛋白含量明显少于其他阶段，差异极显著（P0.01），而其他各阶段的差异不大。

The soluble protein contents at FEC stage were extremely significant ( P0.01 ) lower than that of other stages , but there was little differences at other stages .

采用mRNA差别显示技术对龙眼（DimocarpusLonganLour.）体细胞胚胎发生过程中的胚性愈伤组织、球形胚、鱼雷胚、子叶胚、成熟胚等阶段进行分析。

The mRNA differential display method was applied to analyse differential gene expression in the process of somatic embryogenesis in longan ( Dimocarpus longan Lour . )

不同浓度的2,4-D和6-BA对白皮松胚性愈伤组织的诱导率均存在极显著差异，2,4-D和6-BA的组合存在交互作用并对其胚性愈伤组织诱导有显著影响。

The different concentration combination of 2,4-D and 6-BA , as well as their interaction had a significant effect on induction rates of EC .

胚性愈伤组织转入无2,4-D的分化培养基后能产生发育良好的体细胞胚，再经萌发可得到正常的绿色植株。

After transferred to 2 , 4-D free medium , embryogenic callus could produce well-developed somatic embryos , which readily germinated into normal plants .

低浓度的KT能显著促进小麦幼穗愈伤组织的生长和胚性愈伤组织的形成，而高浓度的KT不利于小麦幼穗体细胞无性系的形成和生长；

In the lower concentration , the KT promoted the formation and growth of wheat immature infloresences regenerated somaclone significantly higher than that of it in the higher concentration .