纯化抗体

方法用RT-PCR方法从能分泌特异性抗人AFP单克隆抗体的杂交瘤细胞中分离纯化抗体VH和VL基因。

Methods VH and VL genes of anti-human AFP monoclonal antibody were cloned by RT-PCR from hybridoma .

用Branford法测定纯化抗体的浓度。

Concentration of the human single-chain antibody was determined by Branford method .

用protein-G亲和层析纯化抗体并用胶体金标记，建立胶体金免疫层析方法，选择最佳反应模式对疟疾血样进行检测。

Protein-G affinity chromato - graphy was employed to purify the antibodies , which were labeled with colloidal gold for establishment of GICA for Plasmodium falciparum detection .

以合成抗原免疫新西兰白兔获得对克百威具高亲合力的抗血清，采用硫酸铵盐析和DEAE纤维素反相吸附法分离和纯化抗体。

The antiserum with high affinity to carbofuran was obtained from New Zealand white rabbits by using synthetic antigen as immunogen . The polyclonal antibodies were separated from antiserum by salting out method and purified by reversed phase adsorption of DEAE cellulose .

用硝酸纤维素膜亲和法纯化抗体

A method for purification of antibodies with protein immobilized on nitrocellulose membrane

结果腹水可以得到1.0～1.5g/L的高效价（10~6）的纯化抗体。

Results 1-1.5 mg high titer ( 10 ~ 6 ) and puri - fied antibody was obtained from ascites .

数天后，技术人员剪下树叶，磨碎，纯化抗体。

After a few days , technicians snip off the plant 's leaves , grind them up and purify the antibody .

单纯疱疹病毒Ⅰ型糖蛋白B胞浆区编码基因的原核表达、纯化及抗体制备

Cloning and prokaryotic expression , purification and amplification to preparing specific antibody of gene encoding herpes simplex virus type ⅰ glycoprotein B cytoplasmic domain

采用间接酶联免疫法（ELISA）对抗血清进行效价检测，通过离子交换层析法获得纯化的抗体。

Antibody was identified by enzyme-lined immunosorbent assay ( ELISA ), and purified by ion exchange chromatography .

重组FAS分子的表达、纯化及抗体制备

Expression and Purification of Recombinant FAS Antigen and the Production of Anti FAS Antibodies

小鼠βNGF纯化、抗体制备和活性鉴定

Purification and Bioassay of Mouse β NGF and Its Antibody

目的：从小鼠腹水和血清中纯化IgG抗体。

∶ Objective ∶ To purify mouse IgG antibody from the ascites fluid and the serum .

鸭MHCⅡα基因的表达纯化与抗体制备

Expression , purification and antibody preparation of duck MHC ⅱα gene

HIV-1病毒感染因子基因克隆、表达、纯化及其抗体制备的研究

Cloning , expression and purification of HIV-1 Vif , and its antibodies production

RAP蛋白的纯化和抗体制备

Purification of RAP and preparation of its polyclonal antibody

牛肾上腺皮质LDL受体的纯化和抗体的制备

Isolation and Purification of LDL Receptor from Bovine Adrenal Cortex and Preparation of Anti-LDL Receptor Antibodies

果蝇中Ecp蛋白的表达、纯化与抗体制备

Preparation of Antibodies Against a Novel Leucine - zipper Protein , Ecp

细胞质型APX的纯化、抗体的制备及放线菌素D对APX活性的影响

Purification of Cytosolic APX from Barley Leaves , Preparation of Antibodies and Effect of AMD on APX Activities

同时，通过亲和层析法和饱和硫酸铵法纯化的抗体经间接ELISA法检测，其效价分别为1：16000和1：32000。

Meanwhile , polyclonal antibody was purified by affinity chromatography and ammonium sulfate precipitation in this article and the antibody titer was detected by indirect ELISA . The purified antibody titers were respectively 1:16000 and 1:32000 .

将经饱和硫酸铵粗纯化的抗体进行westernblot，本底明显降低，抗体损失较小，而以宿主菌丙酮提取物去除本底后，抗体效价大为降低。

After Western blot , the background of antibodies that purified by saturated ammonium sulphate reduced , the loss of the antibodies decreased , nevertheless when used acetone extracts of the host bacteria to remove the background , the antibody titers significantly reduced .

SDS-PAGE结果表明全长基因能高效表达，为后续融合蛋白的纯化和抗体的制备奠定了基础。

SDS-PAGE analysis revealed that the full-length gene could be expressed and the fusion protein was highly expressed , which made it possible to purify the fusion protein and prepare the corresponding antibody in future study .

Hus1（Hus1f）基因可溶性表达、蛋白纯化及抗体制备

Soluble Expression of Hus1 ( Hus1f ) Gene Fragment , Protein Purification and Antibody Preparation

结论冻干Vero细胞纯化疫苗抗体阳转率高、副反应发生率低，临床应用优于地鼠肾纯化疫苗，适宜推广使用。

Conclusion The positive conversion rate of antibody in experimental group after immunized with purified vero cell rabies vaccine for human use is high and superior to the purified shrewmouse kidney cell rabies vaccine , and suitable for popularization .

纯化单克隆抗体在56℃加热5min，即失去抗体活性，而未经纯化的单克隆抗体即使加热30min，抗体活性也毫无损失。

The purified monoclonal antibody completely lost its activity when heated at 56 ℃ for 5 minutes , but the activity of crude monoclonal antibody was stable even heated for 30 minutes at 56 ℃ .

结果纯化的抗体亚类在浓度达到0.5mg/mL时均能在器官培养模型上致表皮产生棘层松解，并能与角质形成细胞膜表面相对分子质量为100000和15000蛋白结合。

Results The acantholysis was induced when the concentration of IgG subclasses reached to 0.5 mg / mL in pemphigus organ culture system . All four subclasses of IgG were reacted with 100 kD and 15 kD proteins on the surface of keratinocytes .

高效凝胶色谱一步法制备级纯化单克隆抗体

One-Step Purification of Monoclonal Antibody on Preparative Scale by Gel Filtration'Chromatography

辛酸沉淀纯化单克隆抗体的研究

A study on Purification of monoclonal antibody by caprylic acid precipitation

镉诱导金属硫蛋白的纯化及其抗体制备

Purification of cadmium induced rat metallothionein and its antiserum preparation

大蹼铃蟾皮肤白蛋白的分离纯化及抗体制备

Purification and Antibody Preparation of Skin Albumin from Bombina maxima

HIV-1tat基因改造及其蛋白表达、纯化与抗体制备

Expression , Purification and Antibody Preparation of a Gene Reconstructed HIV-1 Tat Protein