病毒分离

随机挑选PCR阳性标本2份进行病毒分离试验，2份均为阳性。

Two PCR positive samples were selected randomly for virus isolation test and both were found to be positive .

通过病毒分离和PCR检测，认定了HFMD的病毒病原体。

Virus isolation and PCR identified the viral pathogen of HFMD . 3 .

猪瘟病毒分离株E2基因的克隆与酵母表达系统转移载体的构建

Cloning of E_2 gene of CSFV and construction of Pichia pastoris transfer vector

我国登革2型病毒分离株E蛋白B抗原区的表达与鉴定

Expression and identification of domain B in E protein of dengue virus type 2 isolates in China

新城疫病毒分离株的生物学特性鉴定及F蛋白基因序列分析

Biological Characteristics and Sequence Analysis of Fusion Genes of Newcastle Disease Virus Isolates

应用RT-PCR对新城疫病毒分离株毒力的快速鉴定

Rapid Differentiation of the Virulence of Newcastle Disease Virus Isolates by RT-PCR

1/2例弛缓性瘫痪者PCR和病毒分离阳性。对照CSF均为阴性；

One of two cases of acute flaccid paralysis had PCR and viral culture positive .

北京地区呼吸道合胞病毒分离株G蛋白基因遗传变异特性研究

Study on Genetic Diversity of the G Protein of Subgroups A and B Human Respiratory Syncytial Viruses Isolated in Beijing , China

牛呼吸道合胞体病毒分离鉴定及重组N蛋白间接ELISA诊断方法的建立

Isolation and Identification of Bovine Respiratory Syncytial Virus and Establishment of an Indirect ELISA Diagnostic Method with the Recombinant Nucleocapsid Protein

北京地区TT病毒分离株全基因的克隆及序列测定

Cloning and sequencing of complete TT virus genome from Beijing isolate of China

腺胃病变型鸡传染性支气管炎病毒分离株（IBV-D971）对SPF鸡的致病力试验

Virulence Test on SPF Chickens Using a New Avian Infectious Bronchitis

1983&1984年在南京本校大豆研究室试验田内的广吉品种上分离到K1和K2两个病毒分离物。

Two isolates , K-1 and K-2 , of SMV was obtained from cultivar " Kwanggyo " in soybean fields at Nanjing Agricultural University .

58例孕妇套式PCR阳性率为8.6%，病毒分离阳性率为6.9%。

Among 58 samples of early gravida , the positve rate of nested PCR , virus isolation were 8.6 % and 6.9 % respectively .

在孕龄21周后，通过羊腹腔穿刺术取羊水做病毒分离培养，聚合酶链反应（PCR）分析。

Fetal diagnosis was made after 21 weeks ′ gestation by amniocentesis and based on virus isolation by culture and polymerase chain reaction ( PCR ) .

于感染后不同时间采集小鼠血浆、肝、脾、肾、脑等脏器组织，用病毒分离及RT-PCR方法检测病毒的存在。

The time-course detection of virus in the mouse blood plasma and liver , spleen , kidney , brain samples were observed .

因而PCR是一种可以取代病毒分离的常规测定HIV-1感染的简便方法。

The results suggest that PCR is a simple method instead of virus isolation for the determination of HTV - 1 infection .

鉴定了致病性差异明显的6个大豆花叶病毒分离物，对这些分离物外壳蛋白（CP）基因进行了测序。

Six isolates of soybean mosaic virus ( SMV ) were identified and coat protein ( CP ) gene was sequenced .

实验室2007年的病毒分离率和IgM抗体阳性率都明显低于往年。

The virus isolated rate in Laboratory and IgM-positive rate in 2007 was significantly lower than in previous years .

中国HIV-1病毒分离株p6区的遗传多态性分析

Analysis of genetic polymorphism of p6 region of HIV-1 isolated in China

应用重氮胶乳凝集试验，检测登革热病人急性期血清标本57份，同时与PCR及病毒分离方法进行比较。

Diazotized latex agglutination ( DLA ), polymerase chain reaction and C6 / 36 virus isolation assay were adopted to detect dengue virus infection in 57 sera from the patients .

病毒分离率影响因素的试验结果表明，病毒的分离率与CD4细胞数有明显关系，CD4细胞数越高，分离率越低。

The rate of viral isolation was obviously related to the numbers of CD4 + cells , the lower of CD4 cells , the higher the rate of the viral isolation .

方法：建立套式PCR技术，对105份母－脐配对血进行套式PCR、病毒分离、特异性IgM以及特异性IgA测定。

Methods : Nested PCR was developed . One hundred and five pairs of maternal-infant umbilical blood were analysed by nested PCR , virus isolation and specific antibodies .

本研究利用一步法RT-PCR技术成功扩增了37个新城疫病毒分离毒的（其中2株属于鸽源病毒）F基因片段（约500bp）。

The important function region of the fusion gene of 37 isolates of Newcastle Disease virus was amplified by One step RT-PCR .

L（6565）和SRS瘤株的白血病病毒分离提纯及其生物学特性研究

Isolation purification and biological property of leukemia virus from l_ ( 6565 ) and SRS tumor strains

运用ELISA法检测麻疹IgM抗体、麻疹病毒分离鉴定等方法，结合血清流行病学、病毒学和分子生物学技术，从病原学上明确病因；

Using the serum epidemiology , virology and molecular biology technology to make clear what the pathogen is by detecting IgM antibody by ELISA , measles virus isolation and identification .

[方法]应用实时荧光定量反转录PCR技术，结合HIV病毒分离等方法分析我中心发现的4例HIV抗体确认试验阳性、月龄在18个月以下婴幼儿的HIV感染状况。

Real time RT-PCR and HIV isolation were utilized to determine HIV status of 4 infants who were born to HIV positive mother and all confirmed HIV antibodies positive with aged under 18 months old .

用MDCK细胞进行病毒分离，用血凝抑制试验对分离到的病毒标本进行分型鉴定。

Viral isolation was preformed in MDCK cells and hemagglutination inhibition assay was used for subtype identification .

北京市1998-2004年婴幼儿A3型流感病毒分离株HA1基因序列分析

Sequence analysis of the HA1 regions of hemagglutinin genes of influenza viruses ( H3N2 ) isolated from children in Beijing from 1998-2004

结合病毒分离鉴定确诊为PRRS病毒（PRRSV）感染，RTPCR试验证实分离毒属美洲型PRRSV，且与ATCCVR2332株属同一基因亚型。

RT PCR test proved that the isolate belongs to serotype of American PRRSV and shares the same subgenotype with ATCC VR2332 .

用HSV-1的ACV耐药株感染兔角膜造模，分别用抗生素17997或ACV局部给药治疗，以局部病损及病毒分离判断疗效。

The efficacy of 17997 in treatment of ACV resistant HSV 1 induced rabbit keratitis was evaluated by lesion score and virus isolation .