展示技术

人宫颈癌Hela细胞穿透肽的噬菌体展示技术筛选

Screening of display technology for phages targeting human cervical carcinoma Hela cells-penetrating peptides

目前，mRNA展示技术主要应用于各种靶分子的多肽和蛋白质适体的发现以及蛋白质相互作用机制的阐明和分析。

The application of mRNA display technology is mainly in the discovery of ligands for many kinds of target molecules and the analysis and mechanism elucidation on interaction between proteins .

采用噬菌体展示技术筛选黄曲霉毒素B1模拟抗原表位

Mimic epitope of aflatoxin B_1 screened by phage display technique

噬菌体展示技术筛选干扰素α启动子DNA结合蛋白

Screening of binding proteins to interferon-alpha promoter DNA by phage display technique

运用mRNA体外展示技术筛选胸苷酸合成酶RNA亲和肽

Identification of Peptides That Bind With Thymidylate Synthase RNA Using mRNA Display Technique

mRNA展示技术是一种新兴的体外筛选多肽和蛋白质的有力工具。

MRNA display provides a new powerful tool for in vitro selecting of peptides and proteins .

噬菌体展示技术筛选诱导型NOS基因启动子DNA结合蛋白的研究

Screening of promoter DNA-binding protein of iNOS gene by phage display technique from human liver cDNA library

由于其自身的巨大发展潜力，mRNA展示技术具有更为广阔的应用前景。

With its great potential , there will be a wide application foreground in the application of mRNA display .

利用噬菌体展示技术对突变库进行4轮富集筛淘及ELISA鉴定；以ELISA方法测定所获阳性克隆的特异性。

Hepatoma cell-specific scFvs were selected after four rounds of panning by phage display and ELISA detection .

应用噬菌体展示技术能成功地区分糖尿病相关性IAA与IA的独特型。

Phage display can distinguish idiotypes of diabetes-related IAA from IA .

基于VRML的交互式虚拟产品展示技术

The technology of interactive virtual product exhibition based on VRML

噬菌体展示技术筛选bFGF抗原表位

Screening antigen epitope of bFGF by phage display

噬菌体展示技术筛选DNA错配修复蛋白MutS高亲和性抗体

High affinity antibody on DNA mismatch repair protein MutS through method of phage display

用T7噬菌体展示技术筛选HCV核心蛋白的相互作用蛋白

Screening of proteins interacting with hepatitis C virus core protein from T7-phage display library

应用噬菌体展示技术进行猪瘟病毒结构（糖）蛋白E2和E～（rns）中和抗原表位鉴定和比较

Identification and Comparison of Neutralizing Epitopes of Structural Glycoprotein E2 E ~( rns ) of Classical Swine Fever Virus Using Phage Display

接着，我将向您展示技术债务的两个插图：第一个是手工生成的，第二个是使用Sonar工具生成的。

Next , I 'll show two illustrations of technical debt : the first generated by hand and the second using a tool called Sonar .

M13噬菌体已被广泛地应用于噬菌体展示技术。

M13 bacteriophage has been extensively applied in phage surface display technique .

应用噬菌体展示技术，从猪脂肪细胞免疫的小鼠脾细胞mRNA中构建出单链抗体（scFv）cDNA文库。

Using phage display technology , cDNA library of anti - adipocyte membrane scFv was constructed from mRNA of mice spleen cells , which had been immunized by pig adipocytes .

通过噬菌体展示技术从含高滴度抗乙肝表面抗原（HBsAg）抗体的人外周血淋巴细胞获得抗HBsAgFab抗体的轻、重链基因。

The genes of anti-HBsAg Fab antibody were obtained from human peripheral blood lymphocytes of volunteer with high antibody titer to HBsAg by using of phage display technology .

mRNA展示技术作为一种大容量文库的体外筛选方法，将广泛应用于与固定化靶物质具高度亲和性及特异性的多肽和蛋白质的筛选。

As a novel in vitro selection approach , mRNA display technique would be developed as a powerful tool for isolation of functional peptides and proteins that could interact with immobilized targets with high affinity and specificity .

应用展示技术筛选不同功能的核酸、肽和蛋白是近年的研究热点，目前应用的展示技术包括噬菌体展示技术、核糖体展示技术及mRNA体外展示技术等。

Using display technologies to isolate nucleic acids , peptides and proteins has been studied intensively in recent years . The display technologies now applied include phage display , ribosome display , and in vitro mRNA display .

噬菌体表面展示技术（bacteriophagedisplay）是将外源蛋白或多肽以融合蛋白形式展示在噬菌体的壳蛋白上，并能够保持原有的空间结构和生物活性而受到广泛关注。

The bacteriophage display attracts dramatically attention of people , and it can express inserted gene or a DNA fragment to form fusion capsid protein with primary dimension structure and biological activity .

结论利用噬菌体展示技术筛选到了黄曲霉毒素B1模拟抗原表位，并以其代替黄曲霉毒素B1偶联抗原建立了免疫学检测方法。

Conclusion The mimic epitope of aflatoxin B_1 antigen was obtained successfully by the phage display technigue and it is effective substitutes for aflatoxin B_1-protein conjugate in the immunoassay .

结论利用RTPCR和噬菌体表面展示技术可以成功构建人源性单链抗体库，并达到建库标准，可进一步从中筛选人源性单链抗体。

Conclusions A typical phage display library of human single-chain Fv antibodies has been constructed Using RT-polymerase chain reaction ( RT-PCR ) and phage display technique , human single-chain Fv antibodies can be screened from this library .

目的：通过噬菌体展示技术筛选得到与FGFR结合的bFGF模拟短肽，为bFGF肽类抑制剂的研发提供实验基础。

Objective : To obtain the bFGF mimic peptide binding to FGFR via phage display , and to provide the base for developing peptide agonist of bFGF .

微软最近也借助Silverlight，加入了这场竞赛。这种新型展示技术可以在任何浏览器上运行，堪称对微软传统的一次突破。

Microsoft recently jumped into this game with Silverlight , its new presentation technology that runs in any browser - a break from Microsoft tradition .

用噬菌体表面展示技术构建了ScFv库，可用来筛选人源化抗血小板GPⅡb/ⅢaScFv。

A phage antibody library has been constructed by phage surface display technology . Humanized anti-platelet GP ⅱ b / ⅲ a ScFv phage antibody can be screened from this library .

我们利用噬菌体表面展示技术构建人天然fab段噬菌体抗体库，建立人源抗体制备技术平台，为寻求恶性肿瘤的免疫治疗新方法打下基础。

In this project , we constructed a naive human Fab fragment phage display library , provide a platform for human antibody preparation and a basis to new therapy for the malignant tumors .

文中综述噬菌体、细胞表面、核糖体、mRNA等展示技术的原理和方法，以及其在酶定向进化中的应用。

In this article , we review recent studies on display technology , which include not only the principles and methods of phage display , cell-surface display , ribosome display and mRNA display , but also their application in aspect of directed evolution of enzymes .

通过噬菌体展示技术淘选抗细菌HrpA蛋白的单链抗体。

In this paper , phage display technology was used to screen scFv against HrpA .