定点诱变

再次设计两对引物，通过重叠延伸PCR对该序列进行定点诱变，经测序分析，获得了序列完全正确的PG全长基因。

The full-length gene was site-directed mutagenesis by overlap extension PCR , and the PCR products were sequenced to obtain the correct sequence . 2 .

常用的研究方法有易错PCR，DNAshuffling，定点诱变等等；如姜世民等利用异源DNA同源重组体外定向进化大肠杆菌的p-半乳糖苷酶。

Commonly used research methods are error-prone PCR , DNA Shuffling and site-directed mutagenesis and so on ; such as Jiang Shimin maked heterologous DNA homologous recombination in vitro evolution of E. coli P-galactosidase .

应用USE定点诱变技术制备Wilson病突变体

Construct Wilson Disease Mutant cDNAs with USE Site directed Mutagenesis Technique

结论重组PCR定点诱变技术成功获得突变DNA，合适的细胞稀释有利于转染细胞克隆的筛选纯化。

Appropriate cell dilution is useful to get purified transfected cell clones .

结论：重叠延伸PCR定点诱变技术高效、简便。

CONCLUSION : Overlap-extension PCR method was convenient and efficient for site-directed mutagenesis .

登革2型病毒全长cDNA克隆定点诱变的OL-PCR方法

OL-PCR for site-directed mutagenesis of full-length cDNA clone of DEN-2

藻蓝蛋白裂合酶CpeS色氨酸定点诱变及体内重组功能研究

Site-directed Mutagenesis of the CpeS of Phycocyanin and Study on the Function with Reconstitution in vivo

本研究旨在克隆通城猪含有第1个内含子的MSTN前肽基因，构建真核定点诱变载体，并通过转染C2C12细胞验证载体表达的有效性。

This research intended to construct eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene , and verify its expression efficacy in C2C12 cells .

通过定点诱变结合荧光光谱学方法研究了慈菇蛋白酶抑制剂A和B（APIA和APIB）Trp残基周围构象与酶抑制专一性之间的关系。

The relationship between the micro environment of the two tryptophan residues and the inhibitory specificity of arrowhead protease inhibitors A and B ( APIA and APIB ) was studied by mutagenesis and fluorescence spectroscopy .

利用体外定点诱变技术构建人突变型α-L-艾杜糖醛酸酶基因

Constructing Human Mutant IDUA Gene by in Vitro Site-Directed Mutagenesis Technique

重离子束定点诱变育种初探

Preliminary Research into Site-chosen Mutation with Heavy Ion Beams for Crop Breeding

定点诱变自我剪接Ⅰ型内含子核酶

Self-splicing of the Site-specific Mutagenesis Group ⅰ Introns Ribozyme

猪肌肉生长抑素前肽基因定点诱变载体的构建和功能初步检测

Construction of Site-Directed Mutagenic Plasmid Vector of Porcine Myostatin Propeptide Gene and Preliminary Functional Analysis

乳头瘤病毒表位融合蛋白的基因定点诱变

Site-Directed Mutagenesis of HPV Multi-Epitope Fusion Protein

木聚糖酶基因克隆、表达与分泌及定点诱变研究进展

Recent Advances in Molecular Cloning , Expression , Secretion and Site-directed Mutagenesis of Xylanase Gene Research

蛋白质定点诱变技术的进展

Progress in Protein Side-Directed Mutagenesis

定点诱变用于有计划地改变编码区和非编码区并观察其作用，从而证实基因的作用。

Site-directed mutagenesis is used to deliberately alter these regions and the effects observed , confirming the role of the gene .

为了实现定点定位诱变，提出了一种利用ESR波谱术对离子束Bragg峰在麦粒中的定位方法，即自由基探针技术。

In order to realize site-chosen mutation , the technique of free radical probe i.e.the method of determining Bragg peak location of heavy ion beam in wheat seeds using ESR spectroscopy , has been put forward in the paper .