基质辅助激光解吸电离

直接将细胞色素C吸附在硝酸纤维素（NC）膜上，进行了基质辅助激光解吸电离－飞行时间－质谱（MALDI－TOF－MS）分析。

Cytochrome C absorbed on nitrocellulose ( NC ) membrane was directly analyzed by matrix assisted laser desorption ionization time of flight mass spectrometry ( MALDI TOF MS ) .

基质辅助激光解吸电离（MALDI）技术是近年来发展起来的新的质谱离子化技术。

Matrix assisted laser desorption ionization ( MALDI ) technology is one of the best mass spectrometry ionization technologies for macro molecules which was developed almost two decades ago .

随着基质辅助激光解吸电离质谱（MALDIMS）和电喷雾离子化质谱（ESIMS）两种技术的迅速发展，生物质谱技术已经成为生命科学研究中的重要工具。

With the rapid development of MALDI-MS and ESI-MS , biological mass spectrometry technology has already become an important tool in life science .

应用基质辅助激光解吸电离飞行时间质谱技术研究了激光强度对酸性多肽（acidicpeptide，AP）解吸电离和稳定性的影响。

Effects of laser intensity on the desorption ionization and stability of acidic peptide ( AP ) were studied with matrix assisted laser desorption / ionization time of flight mass spectrometry .

选用基质辅助激光解吸电离化飞行时间（MALDI-TOF）质谱技术分析茶多酚及茶多酚-锗的组成和结构。

Matrix-assisted laser desorption ionization / time of flight ( MALDI-TOF ) mass spectrometry was selected to analyze the composition , structure and stability of tea polyphenol ( TP ) and TP-Ge4 + .

利用海牛卵制备的模拟微量混合内切酶体系，用基质辅助激光解吸电离-飞行时间（MALDI-TOF）质谱技术研究了胰岛素在不同酸碱度和还原条件下的水解和酶解过程。

Using simulated micro mixed endopeptidase system prepared with crushed sea slug eggs , the hydrolysis and enzymolysis of insulin under different pH and reductive conditions were studied by MALDI TOF mass spectrometry .

选取差异蛋白质点，通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）及蛋白质数据库检索进行差异蛋白质鉴定。

The differential proteins in the gel were selected and dug in situ to be identified by matrix-assisted laser desorption ionization time of flight Time mass spectrometry ( MALDI-TOF-MS ) and protein database searching . 2 .

将凝胶中的差异蛋白点切出，经胶内胰蛋白酶解和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）分析获得肽质量指纹谱。

The differential protein spots in the gels were cut , then the peptide mass fingerprint were obtained through In-gel trypsin digestion and matrix-assisted laser desorption / ionization time of flight mass spectrometry ( MALDI-TOF - MS ) analysis . 5 .

结论：基质辅助激光解吸电离质谱为中药中季铵生物碱提供了一种快速分析方法。

For analyzing quarternary ammonium alkaloids , the method is rapid .

生物组织的基质辅助激光解吸电离质谱成像新进展

MALDI imaging mass spectrometry on biological tissue sections : the new progress

基质辅助激光解吸电离飞行时间质谱在蛇毒组分分析中的应用

The Application of MALDI - TOFMS in Snake Venom Analysis

基质辅助激光解吸电离质谱分析糖类物质

Matrix-Assisted Laser Desorption / Ionization Mass Spectrometry ( MALDI-MS ) Application in Carbohydrate Analysis

气溶胶单粒子的检测效率与基质辅助激光解吸电离研究

Investigation of Individual Aerosol Particles Detection Efficiency and Matrix Assisted Laser Desorption / Ionization

结果表明：基质辅助激光解吸电离飞行时间质谱表征三苯胺偶氮化合物时，用咔啉作基质，负离子检测，可以得到较好的质谱图。

The satisfactory mass spectra can be obtained using norharmane as matrix under negative ion detection mode .

液相基质辅助激光解吸电离-傅里叶变换离子回旋共振质谱研究

Studies on the Liquid Matrix Assisted Laser Desorption / Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

黄化质体也有能力合成质体基因组所编码的蛋白质。基质辅助激光解吸电离质谱用于生物组织的质谱成像应用进展

Etioplasts have the capacity to synthesize plastome-coded proteins . Recent Developments in Profiling and Imaging of Molecules from Tissue Sections by MALDI Mass Spectrometry

扼要介绍了基质辅助激光解吸电离/飞行时间质谱，在分析合成高分子中的发展历程、基本原理、主要优点及存在的困难；

This paper briefly introduces the development , basic principles , main advantages and disadvantages , and difficult in analysis of synthetic polymers by matrix assisted laser desorption / ion-ization time-of-flight mass spectrometry .

以2-氨基-5-硝基噻唑为基质辅助激光解吸/电离飞行时间质谱的有效基质分析DNA及蛋白质样品

Analysis of Oligonucleotide and Protein via Matrix-assisted Laser Desorption / Ionization Time-of-flight Mass Spectrometry Using 2-Amino-5-nitrothiazole as an Effective Matrix

目的运用双向电泳和基质辅助的激光解吸电离飞行时间质谱（matrix-assistedLaserDesorption/ionizationtime-of-flightMassSpectrometry，MALDI-TOF-MS）的方法，鉴定年龄相关性白内障和正常晶状体之间差异表达的蛋白质。

Objective To identify the different expressed proteins between the normal lens and age-related cataract by two-dimensional gel electrophoresis ( 2-DGE ) and matrix-assisted laser desorption / ionization time-of-flight mass spectrometry ( MALDI-TOF-MS ) .

香豆素衍生物在基质辅助激光解吸/电离飞行时间质谱测定多肽和蛋白质样品中的应用

Coumaric Derivative Matrices Applied in the Analyses of Peptides and Proteins by Matrix Assisted Laser Desorption / Ionisation Time-of-Flight Mass Spectrometry

差异蛋白经胰蛋白酶酶切后产生肽片段，再利用基质辅助激光解吸／电离飞行时间质谱得到肽指纹图谱来鉴定。

Proteins were identified with peptide mass fingerprinting using matrix-assisted laser desorption ionization time of flight mass spectrometry ( MALDI-TOF MS ) after tryptic in-gel digestion .

采用碱性新基质2-氨基-5-硝基噻唑对DNA和蛋白质样品进行了基质辅助激光解吸/电离飞行时间质谱分析。

Using 2-amino-5-nitrothiazole as a matrix , the matrix-assisted laser desorption / ionization time-of-flight mass spectrometric study of oligonucleotide and protein was carried out .