嘌呤霉素

通过嘌呤霉素敏感试验确定了抑制HEK293细胞生长的最低浓度。

The sensitivity test of puromycin defined the least concentration that resists cell growth .

以[~3H]Leu-LAI为指标，观察放线菌素D和嘌呤霉素对小鼠S（180）腹水瘤细胞特异性iRNA体外转移细胞免疫活性的影响。

The effects of actinomycin D and puromycin on the mediation of cellular immunity by S180 ascitic tumor cell-specific iRNA were investigated .

方法：建立嘌呤霉素（PAN）大鼠肾病模型，用体视学方法检测足突形态改变；

Methods : Puromycin aminonucleoside ( PAN ) nephrosis was established .

我们利用氨基核苷嘌呤霉素（PAN）肾脏损伤模型在小鼠永生足细胞中检验了这些可能性。

We tested these possibilities utilizing the puromycin aminonucleoside ( PAN ) model of renal injury in immortalized mouse podocytes .

目的：观察nephrin在大鼠氨基核苷嘌呤霉素肾病模型（PAN）中的表达变化，探讨其在蛋白尿发生中的作用。

Objective : To investigate the possible role of nephrin in the development of proteinuria in rat with puromycin aminonucleoside ( PAN ) nephropathy .

目的探讨柴黄益肾方提取物（CYE）对嘌呤霉素氨基核苷（PAN）肾病大鼠尿蛋白及纤溶功能的影响。

Objective : To investigate the effects of Chaihuang-Yishen Formula Extract ( CYE ) on urinary protein and the function of fibrinolysis of rats with nephrosis induced by puromycin aminonucleoside ( PAN ) .

给大鼠注射puromycinaminonucleoside（PAN）所建立的肾病模型应该称为嘌呤霉素氨基核苷肾病（PAN肾病），但在国内却多被误为嘌呤霉素肾病。

The nephropathy model induced by puromycin aminonucleoside ( PAN ) in rats should be named puromycin aminonucleoside nephropathy or PAN nephropathy , however , the domestic scholar used to call it as " puromycin nephropathy " .

方法：采用大鼠慢性嘌呤霉素肾硬化模型，随机分为模型组，黄芪当归合剂治疗组（简称中药治疗组），血管紧张素受体拮抗剂（Irbesartan）治疗组（简称AT1RA组）。

Methods : Chronic nephrosclerosis model rats induced by repeated intraperitoneal injection of puromycin were randomly divided into the model group , AAM group and Irbesartan ( an antagonist of angiotensin ) group .

结果经嘌呤霉素筛选得到稳定表达细胞株；

Results Stable express cells were obtained by puromycin selection .

嘌呤霉素肾病鼠足突形态改变的形态计量学研究

Morphometric analysis of glomerular foot process in puromycin aminonucleoside nephropathy in rats

地塞米松对嘌呤霉素损伤小鼠肾小球足细胞系保护作用的分子机制研究

The Protective Mechanism of Dexamethasone in Puromycin Injuried Mouse Podocyte Cell Line

不规则趋化蛋白在嘌呤霉素氨基核苷肾病模型中的表达

Expression of fractalkine in rats with puromycin aminonucleoside nephropathy

泼尼松龙新型靶向脂质体制剂对嘌呤霉素氨基核苷肾病的疗效

Therapeutic effect of a new liposome-prednisolone on the puromycin aminonucleoside nephropathy in rats

用嘌呤霉素稳定筛选16天后，出现细胞克隆株。

A cell clone appeared 16 days after screening .

为提高其水溶性，二盐酸嘌呤霉素设计成其盐的形态。

Puromycin dihydrochloride has been formulated as a salt to achieve higher water solubility .

氨基核苷嘌呤霉素是合成嘌呤毒素的半合成类似物中关键性的中间体。

Puromycin aminonucleoside is the key intermediate in the synthesis of semi-synthetic analogues of puromycin .

采用嘌呤霉素筛选和富集转染具有抗性的细胞。

The antibiotic-resistant transfected cells were selected and enriched by applying puromycin in culture medium .

嘌呤霉素肾病鼠尿蛋白量与足突形态改变的关系

Correlation between urinary protein excretion and morphometric changes of glomerular foot process in rats with puromycin aminonucleoside nephropathy

邻苯三酚红钼络合法与双缩脲比色法测定嘌呤霉素肾病大鼠尿中总蛋白浓度的比较

Comparison between pyrogallol red-molybdate method and biuret colorimetric assay for determining the total urine protein in puromycin aminonucleoside-treated rats

在细胞生物学中用嘌罗霉素来选择用载体转化能表达嘌呤霉素-N-乙酰基转移酶类的哺乳动物细胞系。

Puromycin is used in cell biology to select mammalian cell lines that have been transformed by vectors that express puromycin-N-acetyl-transferase .

氨基核苷嘌呤霉素诱导肾病变是人原发性肾病综合症的模型，抑制培养的肾小球上皮细胞整联蛋白的表达。

Puromycin aminonucleoside-induced nephrosis is a well-described model of human idiopathic nephrotic syndrome , suppressing expression of integrin expression in cultured glomerular epithelial cells .

该盐同嘌呤霉素游离碱具有相同的药理学性质，它的高度的水溶性能给体外研究应用提供一些优势。

While the salt shares the same pharmacological properties as puromycin free base , its greater water solubility may offer advantages in some in vitro applications .

目的：定量研究嘌呤霉素肾病大鼠模型从建立到恢复过程中，足突的形态变化规律。

Objective : To investigate the morphometric changes in the podocyte foot process during the development of , and recovery from , acute puromycin aminonucleoside ( PAN ) nephropathy in rats .

方法：采用嘌呤霉素引起的肾病综合征大鼠模型，以有效的降脂药普伐他汀作阳性对照，观察黄芪当归合剂的作用。

Methods : Effect of AAM was observed in puromycin amino-nucleoside induced nephrotic syndrome model of rat , and pravachol , an effective lipid lowering medicine , was used as Positive control .