原核细胞

趋磁细菌（MB）属变形菌纲，为简单的原核细胞。

Magnetotactic bacteria ( MB ) belongs to Mycetozoan class with simple prokaryotic cell .

即早基因c-fos是广泛存在于原核细胞和真核细胞的高度保守基因。

C-fos , a high conservative gene , resides in generally prokaryotic cell and eukaryotic cell .

方法采取PCR和蛋白质原核细胞表达方法。

Methods PCR and prokaryotic expression technique .

神经元蛋白14-3-3βcDNA克隆及在原核细胞中的表达

CDNA cloning and expression in prokaryotic cells of neuron protein 14-3-3 β

重复序列设计原核细胞差异显示RT-PCR引物研究进展

Progress of Primer Design for Prokaryotic Differential Display RT-PCR

斜纹夜蛾普通气味结合蛋白ⅠcDNA的克隆及在原核细胞中的表达

Cloning and Prokaryotic Expression of cDNA Encoding General Odorant Binding Protein ⅰ from Spodoptera litura

SV40DNAHindⅢB片段对人αD型干扰素基因在原核细胞中表达的增强作用

Enhancing effect of SV40 DNA hind ⅲ B fragment on expression of human α d IFN gene in prokaryotic cells

westernblot结果证实所制备的抗体可有效识别原核细胞表达的及脑组织提取物中存在的1433蛋白。

Western blot assays confirmed that both prokaryotic expressed , and the natively presenting 14 3 3 proteins in the brain extracts were recognized by the prepared antibody .

以原核细胞表达的人PrP蛋白为抗原制备抗人朊蛋白抗体

Preparation of polyclonal antibody to human prion protein using the expressed GST-PrP fusion protein as antigen

单链抗体A7基因的原核细胞表达

Prokaryotic expression of gene of single chain variable fragment A7

目的克隆HGVE2基因并在原核细胞系统中表达HGVE2蛋白。丙型肝炎病毒E2蛋白相互作用蛋白的研究

Objective To clone and express HGV E2 gene . Study on the Interacting Protein with HCV Envelope Protein 2

目的构建人抗HBsAg单链抗体基因，在真核细胞和原核细胞中进行表达，并对其表达产物的活性进行鉴定。

Objective : To construct the single-chain Fv gene of human anti-HBsAg and to analyse the expression of the constructed gene in eukaryotic cell and prokaryotic cell .

为研究丙型肝炎病毒（HCV）包膜蛋白E2在原核细胞和真核细胞中的表达，并对表达蛋白的抗原性进行检测。

Study on protein expression and antigenicity detection of hepatitis C Virus envelope protein E2 in prokaryotic and eucaryotic expression systems . The gene that encoding .

Nogo-66蛋白氨基酸肽的克隆及在原核细胞的表达

Cloning and expression of nogo-66 protein in prokaryotic cells

结论中国旱獭IFNα家族基因能在真核和原核细胞中表达，表达产物具有生物学活性。

Conclusion The IFN α family gene of the Chinese marmot can be expressed in both eukaryotic and prokaryotic cells , and the expression products show antiviral activity in a protection assay .

与原核细胞、酵母细胞以及昆虫细胞相比，中国仓鼠卵巢细胞（CHO）作为宿主细胞表达的外源蛋白最接近其天然构象，因而CHO细胞表达系统是生物工程制药最为理想的表达系统。

Chinese hamster ovary cells ( CHO ) are preferable to prokaryotic , yeast or insect cells as hosts for biopharmaceutical production due to the products are more similar to their natural conformation .

结论：IL-24融合蛋白在原核细胞中高效表达，并获得高纯度、在体外明显诱导乳腺癌细胞凋亡的重组蛋白，为后续的研究提供实验基础。

CONCLUSION : The fusion protein can be highly expressed in E. coli and the obtained protein of high purity can induce tumor cells apoptosis .

植物核糖体灭活蛋白（ribosome-inactivatingproteins，RIPs）能够破坏真核或原核细胞的核糖体大亚基RNA，使核糖体失活而不能与蛋白质合成过程中的延伸因子相结合，从而导致蛋白质合成受到抑制。

The ribosome-inactivating proteins ( RIPs ) from plants damage large ribosomal RNA of prokaryocyte or eukaryocyte , arresting the binding of ribosomes with elongation factors in translation , and inhibiting the protein synthesis .

该研究为PTEN蛋白的抑癌机理和基因工程药物的研究打下了基础，这是国内PTEN蛋白在原核细胞中成功表达的首次报道。

This research has laid foundation for investigating the tumor suppression mechanism of PTEN protein and the probability of genetically engineered medicine of PTEN . This is the first report of PTEN expression in prokaryotic cells in China .

鼠透明带3cDNA的克隆及其在原核细胞中表达的研究

Cloning of Murine Zona Pellucida 3 cDNA and Its Expressing in Prokaryotic Cell

结论：构建成功双启动子表达质粒pCN-SSIE，在体外真核和原核细胞中均能正常表达，用其作为DNA疫苗免疫动物，可诱导明显的免疫应答。

CONCLUSION : The construction of the dual-promoter expression plasmid pCN-SSIE was successful and the plasmid can express in prokaryocyte and eukaryocyte and elicit dramatic immune response when applied as DNA vaccine in experimental animal .

结论P9基因TRAF型锌指结构域编码的蛋白质能够在原核细胞中表达，并能获得具有特异免疫反应性的P9-ZFD多克隆抗体。

Conclusions The protein coded by P9-ZFD can be expressed in prokaryocyte . Polyclonal antibody of P9-ZFD which has peculiar immunoreactive can be prepared .

螺原体是原核细胞生物中最小的一类，可滤过220nm孔径滤膜，它有一种特殊的顶端结构，能使其粘附在宿主细胞表面，这与螺原体的侵染和致病有关。

It has a special tip structure which assists for its adherence to the surface of host cells , which is related to infection and pathogenesis .

结论利用原核细胞表达的GST1433蛋白可有效地诱导免疫动物产生特异性抗体，所制备的抗体可用于CJD病人脑脊液1433蛋白的检测。

Conclusion Using purified prokaryotic expressed GST 14 3 3 fusion protein as antigen , the specific antibody was elicited in the immunized animals . The prepared antibody can be used in identification of 14 3 3 protein in CSF for the diagnosis of CJD .

但5-helix基因在原核细胞中直接表达时易形成包涵体，复性困难，给研究带来不便。

However , 5-helix is apt to be expressed as inclusion body when directly expressed in prokaryocyte and is difficult to renature , which cause inconvenience to future research .

结果表明，在原核细胞中表达了融合蛋白LTB-（Gly4Ser）2-FAP，表达的重组蛋白占菌体蛋白总量的15%。

Induced with IPTG , the recombinant bacterium could express recombinant protein LTB - ( Gly4Ser ) 2-FAP , identified by SDS-PAGE and Western-blot . The total amount of the protein was about 15 % of the total cell protein determined by gel thin scanner .

绦虫催乳素基因克隆及其在原核细胞中的表达

Cloning and Expression of Prolactin Gene of Cestode in Prokaryotic Cell

人神经营养素-4的克隆及其在原核细胞的表达

Cloning and prokaryotic cell expressing of gene encoding human neurotrophin-4

牦牛和双峰驼重组朊蛋白的原核细胞表达

Prokaryotic expression of recombinant prion proteins of domestic yak and Camelus bactrianus

Tα1在原核细胞中的融合表达及活性研究

Fusion Expression and Activity Testing of Thymosin α 1 in Prokaryotic Cells