卵丘

小鼠卵丘透明质酸结合蛋白的分布

Distribution of hyaluronan binding proteins in cumulus oophorus of mice

卵丘是指在卵母细胞外周并与之进行代谢联系的颗粒细胞群；

Cumulus oophorus is a group of closely associated granulose cells , which surrounds the oocyte .

C级为半裸卵，卵母细胞外有部分卵丘细胞存在；

Grade C is hemi-nude egg with sectional cumulus cells ;

B级为卵丘细胞1～3层，基本上包裹卵母细胞；

Grade B have 1-3 layers fully cumulus cells ;

蛋白激酶C和MAPK/p90rsk对小鼠卵丘-卵母细胞复合体减数分裂成熟的调节

Regulation of Protein Kinase C and MAPK / p90rsk Cascade on the Induced Meiotic Maturation in Mouse Cumulus Cell-Oocyte Complexes

此外，我们目前对卵丘细胞促进卵母细胞成熟质量的机制还知之甚少。因此，建立一种完善的DOs体外成熟系统具有非常重要的应用价值和理论意义。

In addition , the mechanisms by which cumulus cells improve oocyte maturation are poorly understood .

MT是调节哺乳动物卵母细胞成熟的重要激素之一，其作用机制可能是通过卵丘细胞实现的。

MT was able to affect mouse oocyte maturation and the regulation mechanisms might be related to cumulus cells .

卵丘颗粒细胞凋亡的检测可以作为预测IVF结果的指标。

The incidence of CGCs apoptosis can be used as an indicator to predict IVF outcome .

但在融合后的桑椹胚/囊胚形成率上，成纤维细胞又明显低于卵丘颗粒细胞和输卵管上皮细胞（P0.05）。

However , the morulae / blastocyst formation rate of fibroblasts was significantly lower than cumulus granulosa cells and oviduct epithelial cells after fused ( P0.05 ) .

这些结果表明，ANP负调节FSH诱导的猪卵母细胞减数分裂恢复、减数分裂成熟和卵丘扩散。

These results suggest that ANP negatively regulates FSH-activated porcine oocyte meiotic resumption , meiotic maturation and cumulus expansion .

EGF虽可促进卵丘细胞的扩展，但对卵母细胞的成熟无剂量依赖性。

EGF had effect on cumulus expansion , but the rate of oocytes was not depend on the EGF dose .

目的采用开放式拉细麦管（openpulledstraws，OPS）法玻璃化冷冻技术，探讨卵丘颗粒细胞对未成熟卵母细胞冻融后存活率及其发育能力的影响。

Objective To study the influence of granulosa cells on the survival rate and maturation rate of immature oocytes after vitrification by open pulled straws .

结果表明，选用胞质均一致密，外围至少有3层以上卵丘细胞包裹的A级卵母细胞，添加浓度为1μg/ml的E2、5μM的？

Oocytes of grade A with uniform and compact cytoplasm and parceled by at least three layers of cumulus cells were chosen .

核移胚以胚胎培养液NCSU23及卵丘单层共培养体系进行分别培养，核移胚卵裂率无明显差异（30.06%、31.5%，P＞0.05）。

Third , when NT embryos cultured in NCSU - 23 , the development rate ( 30 . 06 % ) is no significantly different from those in cumulus single layer culture system ( 31 . 5 % ) .

表明卵母细胞成熟培养过程中，卵丘细胞具有显著抑制IVF胚胎Fas/FaslmRNA表达的作用。

It suggests that the cumulus cells behave evidently inhibitive effects on the expression of Fas / FasL mRNA of IVF embryos during IVM of oocytes .

在IVM临床中常规应用卵丘颗粒细胞单层共培养是有利和可行的。

It is beneficial and feasible to apply cumulus cells monolayer coculture during IVM in clinic routinely .

与Gn联合应用后可进一步促进卵丘卵母细胞复合物胞质的成熟，提高受精率。

EGF combined with Gn can further promote the maturation of OOCs and improve the rate of fertilization .

比较了用转t-PA突变体基因的胎儿成纤维细胞和卵丘细胞作供体时核移胚的发育情况。

The development of nuclear transfer embryos derived from fetal fibroblasts including t-PA gene and ovary granulose cells were compared .

体外成熟培养主要从采集方法、激素配比、性周期阶段、卵丘细胞、添加谷氨酰胺、表皮生长因子（EGF）等方面进行了探讨；

In this experiment , the effect of collection methods , hormone ratio , estrous cycle , cumulus cells , glutamine and EGF were discussed ;

而且，COCs扩展相关基因和IL-6一样，在卵丘颗粒细胞中还是炎症相关基因。

Furthermore , COCs expansion-related transcripts , as well as IL-6 , also serve as inflammation-related genes in cumulus cells .

结果表明，在有腔卵泡的各个时期都有TGFβ2基因表达；在卵泡膜、颗粒细胞和卵丘卵母细胞复合体中也检测到了该生长因子的mRNA。

In different size of goat ovarian follicles and in theca cells , granular cells ( GCs ) and cumulus oocyte complexes ( COCs ), the expression of mRNA of TGF β 2 can all be measured .

ROS和BL-I抑制山羊卵丘扩展，并且转为正常培养后卵丘不能再扩展。

ROS and BL-I inhibited cumulus expansion and the inhibitory effect was not reversible after maturation culture .

本试验主要探讨了促性腺激素（FSH+LH）和17-β-雌二醇（E2）对猪卵母细胞体外成熟的作用以及卵丘细胞群状态与卵核成熟之间的关系。

This experiment was conducted to examine the effect of gonadotropins ( FSH + LH ) and estradiol-17 β( E2 ) on the in vitro maturation of pig follicular oocytes and the relationship between nuclear maturation and morphology of cumulus cells .

处理40h后，Forskolin（3μmol·L-1）的抑制作用被卵丘细胞分泌的某种物质克服；

After 40 hours ' priming , this inhibitory action of Forskolin was overcome by a substance secreted by cumulus cell ;

目的探讨CD44和间α胰蛋白酶抑制剂（ITI）在小鼠卵丘-卵母细胞复合体的表达及两者的相关性。

Objective To investigate CD_ ( 44 ) and inter α - trypsin inhibitor ( ITI ) expression in the cumulus-oocyte complex of mice .

自屠宰场采集猪卵巢，用抽吸法采集卵母细胞，选择外周卵丘细胞包裹致密、卵母细胞胞质均一的卵母细胞（A，B级）进行体外成熟培养，研究其相关影响因素。

The aspiration method was used for recovering the cumulus-complexes ( COCs ) from surface-visible follicles of porcine ovaries from slaughterhouse . Those COCs that have compact cumulus cells and homogeneous oocyte plasma ( A and B grade ) were chosen for in vitro maturation ( I VM ) .

方法小鼠生殖泡期卵丘卵母细胞复合物（COC）或完全去颗粒细胞后的裸卵（DO）用OPS法玻璃化冻存，复苏后行体外成熟培养（IVM）；

Methods The cumulus-oocyte complexes or denuded oocytes ( DO ) at germinal vesicle stage were collected and vitrified , followed by in vitro maturation after thawing .

cGMP类似物8-Br-cGMP（10μM-1mM）可模拟ANP对GVBD、PB1和卵丘扩散的影响。

CGMP analog 8-Br-cGMP ( 10 μ M - 1 mM ) mimicked the effects of ANP on GVBD , PB1 and CEI .

关于卵丘细胞的作用，CEO组的MII期率为68.8%，明显高于DO组的46.7%（P<0.01）；

The MII stage rates of CEO was significantly higher than that of DO group ( 68 . 8 % and 46 . 7 % respectively , P < 0 . 01 ) .

TSG-6和连接蛋白在小鼠卵丘-卵母细胞复合体的共位性分析

Analysis of co-localization of tumor necrosis factor-stimulated gene-6 and link protein in cumulus-oocyte complexes of mouse