第二极体

小鼠第二极体发育潜力研究

Developmental Potential Studies on the Second Polar Body of Mouse

排出极体处的细胞膜微绒毛消失，其下方是中期纺锤体，此区域可能为第二极体的释放部位。

Near the area through which the first polar body was extruded , the microvillus of oocyte disappeared , where the second polar body might possibly be extruded .

采用CB抑制受精卵第二极体排放诱导培育出三倍体种苗7.2×107粒。

Triploidy was induced by inhibiting polar body ⅱ with CB and 7.2 × 10 7 triploid oysters were produced .

6-DMAP诱导太平洋牡蛎三倍体&抑制受精卵第二极体释放

Triploidy of Crassostrea gigas induced with 6-DMAP : by blocking the second polar body of the

用紫外线辐射同源牙鲆精子，利用冷休克抑制第二极体排放使染色体加倍，培育减数分裂雌核发育二倍体。用2只平行的20W紫外灯管，两灯管相距15cm。

Meiotic gynogenesis in P. olivaceus was induced by cold shocks to the eggs fertilized with UV irradiated sperms .

结果如下：①电激活后4h时有78.43%的卵排出第二极体，此后，每小时内的第二极体排出率明显减少；

78.54 % of oocytes extrude the second polar bodies after they were activated by electricity , and subsequently the rate of SPB in an hour dramatically decreased ;

以虹鳟二倍体的伪雄鱼为父本（遗传型为xx），探讨了采用热休克方法阻止第二极体排放诱导全雌虹鳟三倍体的适宜条件。

Sex reversed diploid rainbow trout whose genotype was XX was as father , heat shock was used to restrain the second meiosis of the fertilized eggs of rainbow trout to induced all female rainbow trout triploid .

25～30min排出第一极体；第二极体排出的时间约在授精后55分钟。

By the 25-30th minute postinsemination , the first polar body was ejected . By 55 min after the insemination , the second polar body was released .

结果表明，适当浓度的CHX能提高卵母细胞激活效果，适当浓度的CB可抑制卵母细胞第二极体的排出，提高孤雌激活后双原核的比例。

These results indicate that CHX at an appropriate concentration can improve the parthenogenetic development of buffalo oocytes , CB at an appropriate concentration may inhibit the extrusion of the first polar body and increase the proportion of activated oocytes with two pronuclei . 2 .

哺乳动物第一和第二极体的研究

Study on the First and the Second Polar Bodies in Mammals

第二极体排出的时间约在授精后55分钟。

By 55 min after the insemination , the second polar body was released .

药物直接抑制二倍体马氏珠母贝受精卵第一极体和第二极体的释放，诱导四倍体；

Tetraploidy was induced by inhibition of the first and the second polar bodies in normal diploid zygotes .

受精膜举起后，由次级卵母细胞排放出第二极体。

After fertilization membrane was lifted , the secondary egg emitted the second polar body into the perivitelline space .

卵母细胞在减数分裂过程中相继排出第一和第二极体。

The oocytes undergoing meiotic division sequentially extrude the first and the second small polar bodies from the large oocytes .

采用静水压休克方法研究了促使第二极体保留诱发三倍体水晶彩鲫的最佳条件。

This study was made to optimize hydrostatic pressure shock conditions for producing triploid transparent colored crucian carp ( Carassius auratus transparent colored variety ) by inducing second polar body retention .

九孔鲍受精卵的第一极体、第二极体以及第一次卵裂的出现时间与水温有关。

The release time of first polar body , second polar body and the start time of first cleavage about Haliotis diversicolor aquatilis 's oosperm are related to the water temperature .

受精后卵母细胞表面的微绒毛由倒伏而竖起，第二极体有核，细胞质中细胞器丰富，主要分布于细胞中心。

When the oocytes were fertilized in vitro , the microvilli on their surface became erect , the second polar body had a nucleus , and in the cytoplasm of the oocytes there were abundant organelles which were mainly distributed in the centre of the c ells .