卵白蛋白

选取了三种不同取代度的CMC对卵白蛋白进行糖基化接枝改性，分别对三种CMC的取代度和分子量进行了测定。

CMC with three different degrees of substitution were chosen to modify OVA with the reaction of glycosylation grafting . The degree of substitution and molecular weight of the three kinds of CMC was determined .

鸡蛋清中卵白蛋白和卵类粘蛋白的高效分离

Efficient Separation of Hen Egg White Ovalbumin and Ovomucoid

髓鞘碱性蛋白、卵白蛋白（均为Sigma）。

The myelin basic protein and egg albumen were ( all for Sigma ) .

方法SD大鼠24只，分为哮喘模型组和健康对照组。哮喘模型组大鼠用卵白蛋白（OVA）致敏激发建立大鼠哮喘模型。

Methods A rat asthma model was established by sensitization and challenge with ovalbumin ( OA ) .

哮喘模型制备用卵白蛋白（OVA）致敏大鼠并雾化吸入刺激。

Rats were sensitized and challenged with ovalbumin to establish the asthma model .

1d后卵白蛋白组出现少许轴突回缩球，3d后达到高峰。

After 1 day , cells edema aggravated and axonal retraction ball formed and reached the peach at 3 days in the ovalbumin group .

方法卵白蛋白（OVA）致敏建立小鼠哮喘模型。

Methods A mouse model of asthma was established by sensitization with ovalbumin ( OVA ) .

方法：采用卵白蛋白（OVA）腹腔注射致敏和雾化激发的方法建立小鼠哮喘模型，然后用杏龙平喘方对哮喘小鼠进行干预。

Methods The mouse asthma models were established by sensitization and challenge with ovalbumin ( OVA ) .

哮喘组大鼠采用卵白蛋白10mg腹腔内注射及雾化吸入（5mg）处理建立大鼠哮喘模型；

The animal model of asthma was established by ovalbumin intraperitoneal injection and aerosolization .

结果表明，经UHP处理后S-卵白蛋白的分子量分布没有明显变化。

It was showed no significant change in the molecular weight distribution of S-ovalbumin after UHP under various pressure treatments .

方法36只雌性BALB/c小鼠随机分组，阴性对照组（C组）腹腔注射氢氧化铝凝胶〔Al（OH）3〕；其余用卵白蛋白（OVA）诱导制备哮喘模型。

Methods Among 36 BALB / c female mice , 6 were randomized as negative control group ( group C ), the remaining mice were challenged with ovalbumin ( OVA ) to reproduce asthma .

方法：将小鼠分为对照组（正常）组和哮喘组。急性哮喘小鼠用卵白蛋白致敏及经气道激发，对照组用PBS。

We used mouse model of acute asthma induced by sensitization and airway challenge with ovalbumin and only by PBS in control ( normal ) group .

目的：研究碧云喷雾剂对大鼠卵白蛋白（OVA）变应性鼻炎的作用。

Objective : To study the effects of Biyun spray on the allergic rhinitis ( AR ) in rats induced by ovabumin ( OVA ) .

方法通过卵白蛋白腹腔注射与雾化吸入建立大鼠哮喘模型，并分为哮喘组（A组）、雾化吸入地塞米松组（B组）、正常对照组（C组）。

Methods : An animal model of asthma was established by OVA sensitizing challenge to SD rats . The rats were devided into asthma group ( group A ), dexamethasone aerosol inhalation group ( group B ) and normal control group ( group C ) .

方法采用ELISA法测定卵白蛋白（OVA）致敏小鼠血清总IgE水平；采用大鼠异种被动皮肤过敏反应测定特异IgE水平；

Methods The total IgE level in serum of mice sensitized by OVA was assayed by ELISA and the specific IgE level was evaluated by rat heterologous passive cutaneous anaphylaxis ;

本课题旨在研究母牛分枝杆菌菌苗对卵白蛋白（OVA）致敏的哮喘豚鼠模型的免疫预防作用并探讨其作用机制。

Our objective is to study the immune prophylactic effect of Mycobacterium vaccae vaccine on asthma guinea pig model established by using OVA ( ovalbumin ) .

方法：用卵白蛋白（Ovalbumin，OA）免疫豚鼠使之致敏，以OA攻击诱发过敏性哮喘，观察肝素延长哮喘潜伏期的作用；

METHODS : Guinea-pigs were sensitized by Ovalbumin ( OA ), then , allergic asthma was induced by OA-inhalation and the effect of heparin on prolonging the latent period of asthma was observed ;

方法：体外培养卵白蛋白（OVA）致敏大鼠肺片，在药物处理后以OVA攻击3h，测定肺片VCAM-1的相对表达量。

Methods : In isolated lung slices from ova lbumin ( OVA ) - sensitized rats , the relative expression of VCAM-1 was determined after drug treatment and OVA challenge .

至1d时，卵白蛋白组细胞核变形，亚细胞器亦变形或消失，神经髓鞘分层松解甚至断裂，出现较多的轴索回缩球。

Up to day 1 , nucelus deformation , subneural apparatus degeneration or disappear , myelin sheath delamination and even breakage , and the more axonal retraction balls emerged in the ovalbumin group .

方法：采用生物学方法研究LMWH对卵白蛋白（OA）致敏豚鼠平滑肌的收缩及肺组织液中白三烯（LTs）及NO的影响。

Methods : The effect of LMWH on smooth muscle , leukotrienes ( LTs ) and NO from pulmonary tissue of guinea pigs sensitized by ovalbumin ( OA ) was studied by biological methods .

应用卵白蛋白（OVA）建立哮喘模型，母牛分枝杆菌菌苗组每只豚鼠在OVA致敏前10d肌注22.5μg母牛分枝杆菌菌苗。

A guinea pig model of asthma was formed with ovalbumin ( OVA ) . Every guinea pig of Mycobacterium vaccine group was injected intramuscularly with 22.5 μ g Mycobacterium vaccine 10 days before OVA immunization .

采用不同的酶对鸡蛋蛋清蛋白进行水解，提取卵白蛋白，并通过L9（34）正交试验进行口服液配方研究。

Different enzymes were used to hydrolyze and extract egg white albumin . The optimal formula was determined by the L_9 ( 3 ~ 4 ) design of orthogonal test .

方法：在卵白蛋白（OA）致敏的大鼠，用1%OA气雾攻击后，以计算机辅助技术测定气道管壁面积、支气管周围嗜酸粒细胞数量以及对乙酰甲胆碱（MCh）的反应性。

METHODS : In ovalbumin ( OA ) - sensitized rats , bronchiole wall area , eosinophils around bronchi , and the responses to methacholine ( MCh ) aerosol were measured after 1 % OA aerosol challenge with computer-assisted techniques .

在OVA（卵白蛋白）第二轮激发后小鼠肺中肺泡炎、血管炎迅速加重，BAL细胞总数迅速有力上升，嗜酸细胞比例显著上升，但BAL中的IL-5水平较低。

After second OVA challenge , alveolitis and vasculitis increase rapidly , BAL total counts increase quickly and dramatically , eosinophil ratio increases significantly , but the IL-5 level in BAL is still relatively low .

方法用卵白蛋白和弗氏完全佐剂（OVA+FCA）免疫小鼠制作免疫反应鼠，观察雷公藤组、复方中药组和对照组用药前、用药后小鼠血清IgG水平的变化及脾脏质量和脾脏系数。

Methods The immunological reaction mice were established with OVA and CFA . Serum IgG level change , spleen mass and spleen coefficient of the mice in Tripterygium wilfordii group , complex prescription Chinese materia medica group and control group before and after medication were observed .

整体实验，消咳喘片18mg·kg-1可显著延长卵白蛋白所致的致敏豚鼠呼吸困难，抽搐和跌倒的潜伏期（P<0.01）。

In vivo , Xiaokechuan Tablets at doses of 18mg · kg-1 could significantly prolong the latent period of expiratory dyspnea , tic and falling down caused by egg albumin in sensitized guinea pigs .

目的观察人重组白细胞介素1受体拮抗剂（IL1ra）对正常和卵白蛋白致敏豚鼠离体肺条、气管平滑肌的影响。

AIM To study the effects of recombinant human IL 1 receptor antagonist ( IL 1ra ) on isolated airway smooth muscle tissue .

方法：用10%卵白蛋白液致敏豚鼠，两周后再用1%该溶液雾化吸入，以诱发豚鼠哮喘，测定豚鼠血浆TXB2、6-keto-PGF1α水平。

Methods : The guinea pigs were sensitized with a 10 % albumin solution , and two weeks later 1 % of this solution was atomized and inhaled to induce asthma . The plasma contents of TXB 2 and 6-keto-PGF 1 α were measured .

研究了硫酸铵饱和度对核黄素结合蛋白粗提的影响，采用55%-85%两步（NH4）2SO4盐析，可以去除部分卵白蛋白、卵转铁蛋白和几乎所有的卵黏蛋白。

The effects of ( NH44 ) 2SO4saturation on the crude extracts of riboflavin binding protein was studied , and the results showed that , 55 % - 85 % two step ( NH4 ) 2SO4salting-out can remove part of ovoalbumin , ovotransferrin and almost all of the ovomucin .

糖基化对卵白蛋白分子特性及乳化性的影响

Effect of Glycosylation on Molecular Characteristics and Emulsifying Properties of Ovalbumin