鱼藤酮

HPLC法测定帕金森病大鼠模型中鱼藤酮血浆浓度

HPLC determination of rotenone in Parkinson disease 's model rat plasma

RNA干扰靶向抑制三磷酸甘油醛脱氢酶表达对鱼藤酮所致神经毒性的影响

The effect of targeting inhibition of GAPDH expression by RNA interference on rotenone induced cell death

鱼藤酮对大鼠尾核及血浆中NOS活性和NO含量的影响

Effects of rotenone on NOS activity and NO concentration in caudatum and plasma in rats

目的建立测定帕金森病模型大鼠血浆中鱼藤酮浓度的HPLC方法。

AIM To establish a simple , sensitive and rapid HPLC method for determination of rotenone in Parkinson disease 's rat plasma .

鱼藤酮长期作用对大鼠纹状体单胺递质、NO和NOS的影响

Effects of long-term rotenone intoxication on the monoamine neurotransmitters , NO , and NOS in rat striatum

以NGF诱导后的PC12细胞作为细胞模型，采用鱼藤酮诱导细胞凋亡。

Rotenone was used on NGF-differentiated PC12 cells to induce apoptosis .

在鱼藤酮处理下，纤维细胞中的细胞色素c氧化酶的活性迅速降低，而在硫脲各浓度处理下，绿色棉纤维中的多酚氧化酶活性均降低了80%以上。

Moreover , cytochrome c oxidase activity rapidly decreased in fiber cells after rotenone treatment and polyphenoloxidase activity reduced by more than 80 % in green cotton fiber after thiourea treatment . 4 .

交替氧化酶和可能的内源鱼藤酮不敏感的NADH脱氢酶通过保持ETC的相对氧化状态限制线粒体产生ROS。

The alternative oxidase and possibly internal rotenone-insensitive NADH dehydrogenase limit mitochondrial ROS production by keeping the ETC relatively oxidized .

目的研究鱼藤酮（rotenone）对多巴胺（DA）能神经元的早期毒性作用，并探索一种较理想的组织模型。

Objective To investigate the early neurotoxicity of rotenone on dopaminergic neurons and explore an ideal tissue model .

经诱导生根分化的毛鱼藤愈伤组织鱼藤酮含量达136.2ug／g干重，为愈伤组织含量的7.8倍。

The rotenone content of root & differentiated callus culture of Derris was dramatically increased to 136.2 ug / g dry weight , being 7.8 folds of that of the callus culture .

100nM鱼藤酮处理细胞72h：通过HE染色及免疫细胞化学检测胞浆内的变化；

The changes in cytoplasm were identified with HE staining and immunohistochemistry .

结果表明，毛鱼藤和西非灰毛豆的愈伤组织均能合成鱼藤酮，但含量相当低，分别为17.4ug／g，1.8ug／g干重。

The results showed that rotenone was biologically synthesized by the callus cultures induced from both species though the content of rotenone was low , 17.4 ug / g and 1.8 ug / g dry weight , respectively .

为此本实验进行了相关研究，以寻找鱼藤酮多巴胺能神经元选择性毒性作用机制，藉以探讨多巴胺代谢功能障碍所致多巴胺内源性毒性在PD发病机制中的作用。

Therefore , we carried out some researches in this area , to further investigate the mechanism of rotenone model of PD and to explore the action of dopamine metabolism dysfunction in endogenous dopamine toxicity in PD .

ERK1/2、p38MAPK在鱼藤酮中毒诱导的神经细胞凋亡和iNOS表达中的作用研究

Study on the Role of ERK1 / 2 、 p38MAPK on Neural Cell Apoptosis Apoptosis and iNOS Expression Induced by Long-term Rotenone Intoxication

采用三聚氰胺甲醛树脂（MF）作为囊壁，用原位聚合法对生物农药鱼藤酮进行包囊，制备微胶囊制剂。

The preparation of microcapsules of bio-pesticide rotenone by means of in-situ polymerization , with melamine formaldehyde resin ( MF ) as the shell material , is discussed in this paper .

鱼藤酮大鼠皮下中毒的LD50值为3.601mg/kg，LD50的95%可信限为2.771～4.678mg/kg。

The semi-lethal dose of rotenone for subcutaneous injection is 3.601mg / kg , and the 95 % confident limit is 2.771 ~ 4.678mg/kg .

结论鱼藤酮体外作用可以诱导PC12细胞凋亡以及ERK1/2蛋白磷酸化表达增强。

Conclusions Rotenone could induce apoptosis of PC12 cells , and the expression of phosphorylated ERK1 / 2 proteins is enhanced by activation of MAPK / ERK signal cascade .

在PD研究中有多种动物模型，其中，鱼藤酮能产生与PD流行病学和病理特征更为相似的表现，因而可能在机制上更接近于人类自然病程。

There are many kinds of animal models in PD research , and the PD model induced by rotenone model shows more similar characteristics with epidemiological and pathological features of PD . Therefore , it might be more likely to human natural course of this disease .

点滴处理家蚕三龄幼虫试验表明，毛鱼藤酮的毒力显著高于鱼藤酮，二者的LD50分别为80.07μg/头和244.81μg/头。

The results of topical application bioassay with the third instar silkworm showed that the toxicity of elliptone was significantly higher than that of rotenone . Their LD50 were 80.07 and 244.81ug / head , respectively .

采用动态光散射仪与微电泳仪测定纳米粒的粒径与表面电位；透射电子显微镜（TEM）观察纳米粒表面形态；差示扫描量热仪（DSC）分析纳米粒中鱼藤酮的物理状态。

Diameter and surface potential of nanoparticles was determined by dynamic light scattering meter and micro-electrophoresis meter . The configuration of sample was observed by transmission electron microscope ( TEM ) . With differential scanning calorimetry ( DSC ) the physical state of rotenone in nanoparticles was analyzed .

目的：观察骨髓基质细胞条件液对PC12细胞生长与分化的影响，分析其能否保护PC12细胞免受鱼藤酮的毒性作用。

AIM : To study the effects of marrow stromal cells conditioned medium ( CM ) on proliferation and differentiation of PC12 cells , meanwhile whether CM can protect PC12 cell from the damage of rotenone .

结果：低剂量（5nM和20nM）鱼藤酮处理48小时内对细胞活性无明显影响（P>0.05）；

Results Lower concentrations ( 5nM and 20nM ) of rotenone affected little on cell mortality within 48 hours , but higher concentrations ( > 100nM ) did much .

近年来，Sherer等报道了大鼠背部皮下给予小剂量鱼藤酮，大鼠出现了帕金森病的类似症状和病理特征。

Latterly , Sherer reported that rats who were injected with low dose rotenone subcutaneously developed the similar symptoms of PD and pathological character .

本研究发现：1.大鼠接触小剂量鱼藤酮20d，纹状体内鱼藤酮含量高达3.31ng/g，4～8h仍维持在0.7～0.9ng/g，消除速率较慢。

Our findings suggest that : 1.Rats administrated with low dose of rotenone for 20d , the content of rotenone in striatum is 3.31ng / g , and remaining 0.7 to 0.9ng/g for 4 to 8 h.

鱼藤酮在不同溶剂中的紫外吸收研究

Studies on Ultraviolet Absorption Spectrum of Rotenone in Different Solutions

定氮法测定鱼藤酮辣椒碱悬浮剂中辣椒碱含量

Determination of capsaicin in rotenone-capsaicin suspension by nitrogen determination method

鱼藤酮对星形胶质细胞谷氨酸摄取功能的影响

Effects of rotenone on glutamate uptaking in primary cultured astrocytes

长期低剂量皮下注射鱼藤酮制作大鼠帕金森病模型

Chronic subcutaneous injection of rotenone produces rodent Parkinsonian models

方法：应用D-半乳糖加鱼藤酮建立衰老模型；

METHODS : D-galactose and rotenone were used to establish aging animal model .

经不同浓度鱼藤酮处理后，细胞活力呈剂量依赖性下降；

After exposure to rotenone the cell viability declined in a concentration-dependent manner .