高通量测序

背景：高通量测序已经成为生物学研究的一个越来越重要的工具。

BACKGROUND : High throughput sequencing has become an increasingly important tool for biological research .

高通量测序技术的完善和发展改变了传统微生物学的研究方法，即从原来的已知表型找基因变成了解释已经基因序列的功能。

With the wide applications of high throughput sequencing technology , microbiological research has changed its methodology from phenotype-to-gene to sequence-to-function .

利用高通量测序技术发现植物小分子RNA研究进展

Progress in Plant Small RNA Research via High-Throughput Sequencing

随着新一代高通量测序技术的飞快发展，RNA测序技术（RNA-Seq）已经被广泛应用于各种生物的转录组分析中。

With the rapid development of the next generation sequencing , the RNA sequencing ( RNA-Seq ) is widely used in the analysis of transcriptomes of various organisms .

多种高通量测序平台已足够成熟，被用在解决重大的生物学问题上。

A variety of high-throughput sequencing platforms have matured sufficiently to have been used to tackle significant biological problems .

近年来，随着高通量测序技术的快速发展，基因组学和比较基因组学的研究方法为细菌学领域引入新的研究平台。

Recent years , high-throughput sequencing technologies have developed rapidly , genome and comparative genomics provide insights into latest research platform in bacteriology .

随着高通量测序技术的不断发展，蛋白质序列的数目在呈指数形式增加，但是他们的功能和相互作用关系仍然是未知的。

With the growing development of high-throughput sequencing technology , the amount of protein sequences appears a exponential growth trend . However , their function and interactions are still unknown .

但是，由于高通量测序是一个快速进化的领域，这种静态的方式缺乏容易地采用通常被研究者所需要的最新的进展和技术的能力。

However as high throughput sequencing is a rapidly evolving field , such static approaches lack the ability to readily adopt the latest advances and techniques which are often required by researchers .

本研究首先深度分析中国高通量测序仪的市场特点，市场发展趋势预测，市场的机会和未来可能面临的挑战，同时分析了目前中国客户分类和其消费心理和消费习惯。

This paper deeply analyzed the high throughput sequencing market , including market trend , market opportunities and challenges as well . This paper also analyzed the customer profile , consuming psychology , and spending habit .

但是蝗虫转录组的数据量相对较小，并且通过高通量测序能够得到全基因组范围的基因表达与调控的有效信息。

However , the transcriptome of Locusts has a relatively small amount of data , and through the high-throughput sequencing , we can get the effective information about gene expression and regulation in the whole genome range .

随着基因芯片、高通量测序等现代分子生物学分析手段的不断发展和成熟，棉纤维发育分子机理得到了较为深入的研究，并取得了一系列的重要进展。

With the development of modern molecular biology technology , such as gene chip and high-throughput sequencing , the molecular mechanism of the cotton fiber development has been investigated and a series of important progress have been obtained .

因此，我们采用高通量测序技术对朱砂叶螨进行转录组和数字基因表达谱（升级版）测序，以扩展该物种的基因信息资源，为抗药性研究奠定分子基础。

So , the high-throughput sequencing technology was employed to sequence the CSM transcriptome and Digital Gene Expression Tag Profiling in order to expand the gene information resources of the species and lay a molecular foundation for future study of insecticide resistance .

寻求在中国使用我们高通量基因测序专利技术，从事遗传病诊断业务的投资者。

Seeking investors to engage in genetic disease diagnosis business in China by using our patented high-throughput gene sequencing technologies .

边合成边测序的高通量基因测序技术存在一个相位问题，即碱基合成的先后顺序会出现“超前”和“延迟”。

The high-throughput genome sequencing has a phase problem , i.e. " exceeding " or " delaying ", which appears in the order of bases synthesizing .

由于新一代测序技术的低成本和高通量，测序技术也将广泛利用到微生物研究领域。由核酸序列数据可能成为微生物的研究成本最低也最为广泛使用的数据类型。

As the emergence of Next Generation sequencing technology , the high throughput and low cost facilitates the use of sequencing in microorganism study , and genome sequencing might be the most widely used data in microorganism study .

首先发展起来的高通量技术是测序的方法。

The first high-throughput techniques to be developed were sequencing methods .

随着高通量、短序列测序科技的出现，测序覆盖度进一步提高，这给原有的序列拼接技术带来了严峻的挑战。

With the appearance of high throughput short read sequencing technology , the sequence genomes achieve a higher coverage , which brings serious challenges for the original sequence assembler .

我们以高通量寡聚核苷酸合成和高通量测序为基础，找到一种构建高质量突变库的方法。

We develop a new strategy to construct the mutant library based on high-throughput oligo synthesize and high-throughput sequencing .