鞘糖脂

人精子中性鞘糖脂的纯化与分析及其抗体的制备与鉴定

Purification and Analysis of Human Sperm Neutral Glycosphingolipid and Preparation and Characterization of Its Antibody

人精子鞘糖脂组分的纯化与分析

Purification and analysis of human sperm glycosphingolipid

目的探讨N糖基化抑制对肿瘤细胞中性鞘糖脂（NGSLs）合成的影响。

To study the effects of N-glycosylation inhibition on the expression of neutral glycosphingolipids ( N-GSLs ) in cells .

说明GlobN为一类中性鞘糖脂，其结构与组织来源相关。

It suggested that Glob-N is a kind of neutral glycosphingolipids , and its molecular structure is different in different tissues .

肿瘤耐药相关鞘糖脂CMH对人树突状细胞B7表达的影响

Influence of tumor MDR associated CMH on the expression of B7 in DC

神经节苷脂（Gangliosides）是一类含唾液酸的鞘糖脂，存在于细胞膜的外表面。在胚胎形成、细胞分化、细胞增殖、转化和细胞转移等生理现象中发挥着重要作用。

Gangliosides ( Sialic acid-containing glycosphingolipids ) are involved in embryogenesis , cell differentiation , cell proliferation , transformation and cell metastasis .

CMH为KBv（200）耐药相关中性鞘糖脂

CMH is the multidrug resistance ( mdr ) associated neutral glycosphingolipid in kbv_ ( 200 ) cell line

在细胞表面，GPI锚定蛋白常与鞘脂（包括鞘糖脂和鞘磷脂），胆固醇等聚集在一起形成具有特定结构和功能的质膜微区，称GPI微域（GPImicrodomain）。

GPI-anchored proteins often gather with sphingolipids ( including sphingomyelin and glycosphingolipid ) and cholesterol to form special plasma membrane microdomains ( GPI microdomain ) .

方法人脑中性鞘糖脂经过加压柱层析，梯度甲醇/氯仿溶液洗脱。对所得到的产物行1H-NMR和13C-NMR测定。

Methods Neutral glycosylsphingolipid was separated from normal human brain by column chromatography with methanol / chloroform solution for gradient elution , and the resultant underwent 1H-NMR and 13C-NMR analyses for identifying the structure of Glob-N.

方法本实验运用改良的Hakomori的方法提取、纯化了脑胶质瘤及胚胎脑的中性鞘糖脂，并行高效薄层层析（HPTLC）。

METHODS A modified Hakomori ′ s method was used to extract and purify the N GSLs from glioma and embryonic brain tissues separately , and analyzed by high performance thin layer chromatography ( HPTLC ) .

人正常子宫肌肉的鞘糖脂组成及其在不同生理功能阶段的变化

Changes of Glycosphingolipids in Different Physiologically Functional States of Human Myometrium

中性鞘糖脂也有明显变化。

Meanwhile , neutral glycosphingolipids also showed some obvious alteration .

鞘糖脂与细胞凋亡的关系及其在肿瘤治疗中的意义

Glycosphingolipids and apoptosis : role in cancer therapy

肝癌大鼠各组织中的鞘糖脂图谱

Glycosphingolipids patterns in tissues of rats with hepatoma

妊娠期家兔子宫内膜的鞘糖脂表达

Glycosphingolipid Expression of Rabbit Endometrium during Pregnancy

脑胶质瘤中性鞘糖脂抗原的研究

Study on the neutral glycosphingolipids in gliomas

一种新的中性鞘糖脂显色方法

A new method of visualizing neutral glycosphingolipids

人子宫平滑肌肉瘤中性鞘糖脂含量、组成及其四糖基组分糖链结构的变化

Changes of Total content , Composition and Carbohydrate Chain Structure of Neutral Glycosphingolipid in Human Leiomyosarcoma

肿瘤相关中性鞘糖脂的实验研究

Investigation on tumor associated neutral glycosphingolipids

免疫活性细胞和肿瘤耐药细胞的中性鞘糖脂表达与肿瘤免疫的相关关系研究

Study on the Relationship of N-GSLs Expression of Immunocompetents Cells and MDR Tumor Cells & Tumor Immunology

类鞘糖脂化合物是根据天然鞘糖脂结构合成出来的一类具有生物活性的重要化合物。

Analogues of glycosphingolipids synthesized by natural glycosphingolipids are associated with a wide variety of biological activities .

人正常生育期子宫肌肉的鞘糖脂组成

Glycosphingolipid of human uterus

米非司酮、炔诺酮、三苯氧胺对人早孕绒毛鞘糖脂的影响

The Influences of Mifepristone 、 Norethisterone and Tamoxifen on the Glycosphingolipids Compositions from Human Chorionic Tissues during Early Pregnancy

凋亡相关蛋白、中性鞘糖脂与人卵巢癌细胞株COC1/DDP多药耐药相关性及逆转的研究

Study of Correlation of Apoptosis Related Protein , Neutral Glycosphingolipids and Multidrug Resistance in Human Ovarian Cell Line COC1 / DDP

正常脑与脑胶质瘤及胚胎脑的中性糖脂的表达不同，可能存在胶质瘤相关胚胎鞘糖脂抗原。

The expression of N-GSLs is different in glioma , adult and embryonic brain , CTH may be the glioma associated embryonic antigen .

其特征是患者细胞中胆固醇和鞘糖脂等脂类沉积在晚期胞内体/溶酶体中。

At the cellular level , the disease is a result of improper trafficking of lipids such as cholesterol and glycosphingolipids ( GSLs ) to a late endosome / lysosome compartment .

膜表面会发生侧向相分离，特定的脂质成分（鞘糖脂、胆固醇和鞘磷脂）形成微小的脂质功能区&脂筏。

Lateral phase separation exists on the surface of bio-membrane , and specific lipids ( GSLs , SM and Chol ) form small lipid domains with biological function - " Rafts " .

用不同的鞘糖脂合成抑制剂下调鞘糖脂的表达可以抑制多种肿瘤细胞株的生长和分化，并在裸鼠试验中有抗肿瘤作用。

Down regulating GSL expression by different inhibitors can cause cell growth arrest and differentiation in a variety of cancer cell lines , which has been verified with antitumor effect in nude mice experiments .

鉴于子宫肌瘤是激素依赖性肿瘤，这些鞘糖脂组分的改变在肿瘤发生过程中起什么作用，以及它们是否与女性激素作用有关，有待进一步研究。

Considering that uterine leiomyoma is a hormone-dependent neoplasm , further work should be carried out to see what role these variations in the composition of glycosphingolipids would play in developing this kind of neoplasm , and whether estrogen is involved in it .