非同源末端连接

哺乳动物细胞DNA非同源末端连接及其生物学意义

Mechanism of Non - homologous End Joining and Its Biological Implications

哺乳动物细胞有两种重要的DNA双链断裂修复方式：非同源末端连接和同源重组。

Mammalian cells accomplish DSBs via two pathways : non-homologous end joining and homologous recombination .

V（D）J重组与DNA非同源末端连接

V ( D ) J recombination and DNA non-homologous end joining

结论无细胞的非同源末端连接检测体系的建立是研究人类白血病细胞DNA修复机制的实验基础。

Conclusion Cell-free non-homologous end joining system has been developed to support the study of mechanism of DNA repair in human leukemic cells .

非同源末端连接途径除了在DNA双链断裂修复中起重要作用外，在V（D）J重组、HIV-1病毒整合宿主基因，以及假基因和重复序列的插入上也起着重要作用。

Apart from DNA repair , non-homologous end joining ( NHEJ ) may play a role in V ( D ) J recombination , and integration of HIV-1 genome into a host genome , as well as the insertion of pseudogenes and repetitive sequences into the genome of mammalian cells .

用于临床标本的无细胞非同源末端连接检测体系的建立

Development of a Cell-free System for Detection of Non-homologous End Joining Efficiency in Clinical Samples

目的建立一个用于临床标本的体外非同源末端连接的检测体系。

Objective To develop an in vitro system for non-homologous end joining efficiency detected in clinical samples .

结论推测重复序列、断裂点附近较强的拓扑异构酶Ⅱ酶切位点关联易引起基因的断裂重组，加上非同源末端连接修复机制等综合因素可能是导致基因缺失的重要原因。

Conclusion Repeat sequence and strong topoisomerase ⅱ cleavage site around the breakpoint may predispose double-strand DNA breaks and recombination , which , in addition to the nonhomologous end-joining mechanism , may contribute as important factors to the gene deletion .

同源重组（HR）和非同源性末端连接（NHEJ）是DSB的两条重要修复途径。

Homologous recombination ( HR ) and the unusual origin end joining ( NHEJ ) are two major pathways to repair DSB .