锁状联合

研究证明根据细胞外氧化酶，菌落颜色，菌丝类型、锁状联合有无及分布、菌丝分化特殊结构等微观形态是重要的鉴定分类依据。

The results show that extracellular oxidase , color of mycelial mat , and microscopic morphological characters of wood-decaying fungi such as m ~ hae type , existance and distribution of clamp connection , special construction formed during mycelia differentiation , are important classification and identification foundations .

培养约6天，两条初生菌丝通过顶端细胞或其他部位融合，发育成具有顶端分生孢子和锁状联合的次生菌丝体；

After being cultivated for about 6 days , two primary mycelia fused through terminal cells or other parts and developed into secondary mycelia with clamp connection and conidiospore .

杂交试验表明，出发菌株IM02、突变株A1与金针菇单核菌丝IS02均形成了锁状联合。碘液染色检查发现绝大多数花粉不育，并与该突变株的低结实率一致。

The authenticity of the mutant strain was confirmed by the hybridation test . When staining with iodic solution , most of the pollens showed sterility , which was in consistence with the low setting percentage of the mutant plant .

试验共获得209株再生菌株，通过核染色检测，共获得37株具有双核和锁状联合的再生株。

209 regeneration strains has been obtained . Thirty seven regeneration strains has dicaryotic and clamp cell by dyeing of cell nucleus .