酸性蛋白酶

产偏酸性蛋白酶菌株的筛选及其酶应用特性的研究

Studies on Screening of the Strain which Produced Partial Acid Protease and Enzymatic Application Properties

中性和酸性蛋白酶对血清中葡萄糖、总蛋白及尿素氮含量存在显著的互作效应（P0.05）。

Neutral and acid protease there is a significant interaction effect ( P0.05 ) .

菌丝相白念珠菌分泌性酸性蛋白酶与细胞外磷脂酶的活力均显著高于孢子相（P分别<0.001和<0.002）；

The activities of secretory acid proteinase and extracellular phospholipase in hyphal forms were significantly higher than that of yeast forms ( P < 0.001 );

米曲霉（AspergillusOryzae）A－9005酸性蛋白酶的初步研究

A Preliminary Study on Acid Proteinase from Aspergillus Oryzae A-9005

最后，将首次提出的用加压CO2从混合溶剂中沉淀蛋白质的新方法，运用于纤维素酶分离纯化和酸性蛋白酶、碱性蛋白酶去除杂蛋白过程。

Finally , the new process with precipitating of proteins from mixed solvent using compressed carbon dioxide was applied to purification of cellulase , acid proteinase and basic proteinase .

试验结果表明，酒用酸性蛋白酶的适宜作用温度为30～55℃，适宜作用pH为2.0～6.0，适合于在酒精生产中应用。

The result of experiment showed that the suitable action temperature is 30 ~ 55 ℃, and the suitable action pH is 2.0 ~ 6.0.The acid protease is suitable for alcohol production .

在30℃的最适温度下，3株菌种混合进行固体发酵，粗蛋白增加量（绝干）约为16%～18%，酸性蛋白酶活性5139u／g，纤维素酶活性达61u／g。

At the optimal temperature 30 ℃, the total protein content increased about 16 % ~ 18 % , acid proteinase activity was 5139 u / g. cellulase activity was 61 u / g .

由黑曲霉CPu菌株用60Coγ射线诱变处理，经初筛、复筛，获得5株酸性蛋白酶变异菌株。

Five strains of acid protease are obtained by the method of 60Co γ - rays inducing treatment on the Aspergillus niger cellulase strain CPu .

试验组饲喂基础日粮+酸性蛋白酶0.1%，试验期28d。

Basal diets added acid protease 0.1 % were fed to the experimental group for 28 d.

青霉（Penicillium）P-1007产酸性蛋白酶的条件和酶学性质

The conditions and the nature of enzyme of the acid proteinase produced by Penicillium P-1007

其中添加80mg/kg的中性蛋白酶和40mg/kg的酸性蛋白酶作用效果最佳，而单独在饲料中添加80mg/kg酸性蛋白时料重比最高（P0.05）。

Which add 80mg / kg neutral protease and acid protease effect of the best , but only in the diet containing 80mg / kg acidic protein , the highest feed conversion ratio ( P0.05 ) .

本文用自制酸性蛋白酶粗酶液降解壳聚糖，研究了降解过程中温度、pH值、反应时间、酶用量及壳聚糖脱乙酰度（DD）对酶降解能力的影响。

Partially N-acetylated chitosan was degraded by a homemade and efficient acidic proteinase . The effects of reaction temperature , pH , time , enzyme concentration and DD of chitosan on the hydrolysis of chitosan were investigated .

试验结果表明，吐温-80的添加对提高黑曲霉产酶效价有一定的效果，并且在其浓度为5.8%，添加时间为60小时，培养基pH为5.4时，酸性蛋白酶的活力达到最大值。

The result shows that the addition of Tween-80 can improve the enzyme producing efficiency , and the activity of acidic protease is at its maximum when the concentration of oxygen-vector is 5.8 % , adding time is 60 hr and medium pH is 5.4 .

病例组分泌型酸性蛋白酶活性Pa值为0.490±0.053，低于健康组的0.610±0.056（P<0.01）。

The activity of secreted aspartic proteinase ( Value Pa ) was 0.490 ± 0.053 in patient group and 0.610 ± 0.056 in healthy group . The difference between two groups was significant , too ( P < 0.05 ) .

同时也考察了添加酸性蛋白酶对DDGS品质的影响，结果表明酸性蛋白酶的使用并不减少DDGS的蛋白含量，而且增加蛋白质含量。

The quality of DDGs when adding acid protease was also studied and found out that the protein content of the DDGS was not reduced but increased .

对青霉（Penicillium）P-1007产酸性蛋白酶固体发酵条件优化和酶学性质进行了研究。

The optimization of solid-state fermentation conditions for production of acid protease by Penicillium P-1007 and the enzyme property were studied in this paper .

青霉P-1007酸性蛋白酶酶学性质及其固体发酵条件的初步研究

Preliminary Study on the Zymological Properties of Acid Protease Produced by Penicillium P-1007 and Its Solid Fermentation Conditions

酸性蛋白酶pepB基因植物表达载体的构建及在玉米中的转化

Construction of Expression Vector for pepB Gene of Acidic Protease and the Genetic Transformation in Maize

与相同条件下不使用酸性蛋白酶的比较，发酵周期缩短10～16h，相对原料出酒率提高2.99%～5.73%

As compared with no adding acid protease , the fermentation time was decreased 10 ~ 16h and the alcohol yield of raw materials was increased by 2.99 % ~ 5.73 % .

综合评价工艺条件以及功能实验结果得出，采用537酸性蛋白酶可以简化工艺，对于水解度为16%的酸性蛋白酶酶解产物，蛋白浓度为1mg/mL时，对ACE的抑制率为71.71%；

The application of 537 acidic protease could simplify the process for the production of bioactive peptides from oyster . For the hydrolysate with a DH of 16 % prepared with 537 acidic protease , the inhibiting rate on ACE was 71.71 % at a protein concentration of 1mg / mL.

以麸皮和豆饼粉为主要底物，30℃混合固态发酵36h，获得了酸性蛋白酶活力1440U/g，酵母菌数6.29×109个/g，粗蛋白质70.56%，小肽10.12%，还原糖8%的新型蛋白饲料。

So a new protein feed with acid protease activity 1 440 U / g , number of yeasts 6.29 × 109 / g , crude protein 70.56 % , small peptide 10.12 % and reducing sugar 8 % was obtained .

通过黑曲霉固体发酵生产酸性蛋白酶，对此酸性蛋白酶的部分酶学稳定性（耐热、耐酸性、金属离子稳定性等）进行实验探讨。在不同pH的Tris-HCI中孵育一定时间，评价其耐酸性；

The stability of Acid protease produced by Aspergillus niger was studied , including heat stability , acid stability , metals ion stability , etc . VacA-IgY was added into Tris-HCl of different pH values and incubated under a temperature of 37 ℃ to access its acid stability using ELISA .

酒精发酵中应用酸性蛋白酶的研究

The Study on the Application of Acid Proteinase to Ethanol Fermentation

酸性蛋白酶高产菌株的选育

Breeding of the Strain for Producing High-Yield Acid - Resistant Protease

响应面法优化米曲霉酸性蛋白酶的固态发酵培养基

Optimization of solid-state fermentation medium for acid protease of Aspergillus oryzae

对酸性蛋白酶在酒精发酵中的应用进行了研究。

The application of acid proteinase to ethanol fermentation was studied .

高活力酸性蛋白酶菌株在酱油酿造中的应用

The Application of High-Activity Acidic Protease Strain in Soy Sauce Brewing

白色念珠菌分泌型酸性蛋白酶的诱导研究

Studies on induction of secretory acid proteinase in candida albicans

皮革用酸性蛋白酶的研究与开发

Studies and developments on the acid protease for leather processing

米曲霉A－9005产生酸性蛋白酶的适宜条件

Suitable Conditions for the Production of Acid Proteinase by Aspergillus Oryzae A-9005