近端小管

甲苯胺兰（TB）反应显示了近端小管、中间段和集合管上皮中的亮、暗细胞。

Toluidine blue ( TB ) stained the bright , dark cells of the proximal tubule , intermediate segment and collecting duct epithelium .

目的：创建一种重复性好、细胞量多的人肾近端小管细胞（PTC）培养方法。

Objective : To establish a duplicable and efficient culture method of human proximal tubule cells ( PTC ) in vitro .

蛋白激酶C在高糖诱导近端小管上皮细胞细胞外基质及转化生长因子β1高表达中的作用

Role of protein kinase C on high glucose-induced extracellular matrix and transforming growth factor β 1 expression in renal proximal tubular epithelial cells

大黄抑制近端小管和髓袢升支粗段小管Na~＋－K~＋ATP酶活性

Rhubarb inhibits Na ￣ + - K ￣ + ATPase activities of rat proximal and medullary thick ascend-ing

肾脏近端小管上皮细胞ATP缺乏是研究肾脏缺血时多种细胞骨架成分及其功能改变的良好模型。

ATP-depletion is a ideal model for studying various cytoskeletal and functional alternations induced by renal ischemia in renal proximal tubules ( PT ) .

目的：研究的目的是阐明在ATP缺乏的免近端小管（PT）中，自微绒毛内肌动蛋白细胞骨架上崩解的肌动蛋白是否与非肌动蛋白结合。

Objective : To demonstrate whether the actin breakdown from actin-cytoskeleton in microvilli was bound to any non-actin protein ( s ) in ATP-depleted rabbit proximal tubules ( PT ) .

我们以往的研究表明，肾脏近端小管上皮细胞ATP缺乏时，从微绒毛脱落的肌动蛋白是和非肌动蛋白结合在一起的。

Our previous study showed that the actin broken down from the microvillar F-actin filaments was bound to non-actin protein ( s ) in pellet of renal PT during ATP-depletion .

ATPase在离子的跨膜转运及膜电位的调节方面起着关键作用，主要分布在近端小管刷状缘，而在中间段腔面为弱阳性。

ATPase in the transmembrane transport of ions and the regulation of membrane potential plays a key role , mainly brush border of the proximal tubule , and in the intermediate segment though only weakly positive .

在7～30d、70～140dc－Myc、c－Fos、c－Jun、c－H－Ras分别在近端小管、髓襻、远端小管、集合管上皮细胞内表达。

RESULTS : c-Myc , c-Fos , c-Jun and c-H-Ras were expressed in the epithelial cells of the proximal tubules , the medullary loops , the distal and collecting tubules in 7 ~ 30 and 70 ~ 140 days , respectively .

切应力对高糖培养大鼠肾近端小管上皮细胞纤溶酶原激活物（tPA）及其抑制物（PAI-1）表达的影响及其意义

Effects of Shear Stress on Expression of Tissue Plasminogen Activator ( tPA ) and Plasminogen Activator Inhibitor-1 ( PAI-1 ) of Rat Kidney Proximal Tubular Epithelial Cells Cultured in High Glucose and Its Significance

碱性磷酸酶（ALPase）存在于物质交换活跃的部位，主要分布在近端小管刷状缘。

Alkaline phosphatase ( ALPase ), distributed only in brush border of the proximal tubule , its activity exists in the material exchange site .

集合管腔面显示PAS强阳性，血管球、远端小管细胞侧面、近端小管刷状缘为阳性，其它部位反应较弱。

Luminal surface showed strong set of PAS-positive . endothelium of the glomerular capillaries , the distal tubule cells , and the brush border of proximal tubule cell were positive , while in other parts the response is weak .

ERK参与转化生长因子β-1（TGF-β1）或醛固酮诱导的近端小管细胞EMT及肾纤维化。

It has been proven that of ERK was involved in the transforming growth factor - β 1 ( TGF - β 1 ) or aldosterone-induced EMT of the proximal tubular epithelial cells and renal fibrosis .

【结果】①所有肾病综合征及急性肾炎肾病理切片均存在不同程度PAX2再表达，且其中肾远端小管表达明显高于近端小管（P0.05），足细胞表达微弱。

【 Results 】① PAX2 re-expression was showed in all sections of NS and AGN , more intense staining in distal tubules than in proximal tubules , in podocytes faintly . Normal control was negative .

PAS反应用来鉴定碳水化合物和大分子的糖结合物，在中华鳖肾脏中的分布范围广泛，主要分布于肾小球、近端小管、集合管的上皮细胞及所有肾小管的基底膜。

PAS reaction , used to identify the carbohydrates and the macromolecular glycoconjugates , is located throughout the kidney of soft-shelled turtle , mainly in the glomeruli , proximal tubules , collecting duct epithelial cells , and all tubular basement membrane .

琥珀酸脱氢酶（SDH）为细胞内线粒体的标志酶，其主要分布在中间段、远端小管和集合管上皮细胞中，而在肾小球、近端小管中的反应较弱。

Succinate dehydrogenase ( SDH ) as a sign of mitochondrial enzymes in cells , its main distribution in the intermediate segment , distal tubule , collecting duct epithelial cells and in the glomeruli , but in the proximal tubule the response was weekly positive .

本研究用原代培养的人肾近端小管细胞构建成功RAD，将其应用于整体动物MODS猪模型，观察疗效，为进一步临床试用RAD提供必备的技术平台和实验数据。

This study was to investigate the effect of RAD containing human renal proximal tubule cells ( hPTC ) on porcine model with MODS and ARF . It is necessary to get this date for further clinical application of RAD .

结果：CP组鼠引起肾皮质损伤，可见肾小体肿大毛细血管基膜损伤，近端小管上皮浊肿、空泡形成乃至细胞脱落死亡。

Results : The kidney cortex were damaged , swelled renal corpuscle , vacuoles even necrotic cells of proximal tubules were observed throughout the cortex , basement membrane of the capillary of the renal corpuscle and proximal tubules were also damaged , CP 2 group more serious .

另外，BDCM通过抑制E-cadherinmRNA和蛋白的表达和在转录水平上激活Wnt通路下游基因c-myc和cyClinD1来引起近端小管上皮细胞异常增生。

In addition , BDCM induced an elevation of cell proliferation in the epithelial cells of the proximal tubule via suppression of E-cadherin mRNA and protein expression and transcriptional activation of c-myc and cyclin D1 , downstream genes of the Wnt pathway .

PTCs密度与血肌酐（Scr）水平、肾间质面积呈负相关（P<0.001），与近端小管面积呈正相关（P<0.001）。

The PTCs density was positive correlation with the level of serium creatine and the area of renal tubule , but negative with the area of renal interstitial ( all of P values were less than 0.01 ) .

在发育后期阶段近端小管和远端小管PAX2表达进行性减少，少数仍存在远端小管微弱表达，集合管表达持续存在。

Attenuation of PAX2 was detected in tubules in later stage of renal development , but a few of distal tubules and collecting tubules expressing continuously .

肌球蛋白Ⅵ（myosinⅥ，由Myo6基因编码）在肾脏近端小管细胞中大量表达，是迄今为止发现的唯一能够朝着沿细胞核方向运动的肌球蛋白。

Myosin VI ( encoded by Myo6 ), which the only myosin known to move towards the minus end of the actin filament thus far , is highly expressed in renal proximal tubule cells .

虽然AT2受体在肾脏的近端小管、远端小管和血管系统都存在表达，AT2受体刺激诱导肾脏的缓激肽（bradykinin）/NO通道，但很少有关AT2受体调节肾功能和钠外排方面有价值的研究。

Although the AT_2 receptor is expressed in renal proximal and distal tubules and vasculature , and AT_2 receptor stimulation induces a bradykinin / NO pathway within the kidney , little information is available concerning AT_2 receptor regulation of renal function and sodium excretion .

近端小管表面细胞膜再循环的直接证据

A direct evidence for membrane recycling of rat renal proximal tubule cells

方法：在体外建立原代兔肾近端小管细胞培养。

Meth-ods : Primary culture of rabbit proximal tubular cells was established .

人肾近端小管上皮细胞的原代培养、传代及鉴定方法研究

Primary Culture , Subculture and Identification of Human Renal Proximal Tubule Cells

慢性肝病者的肾小管酸中毒猪肾近端小管的超微结构

Renal Tubule Acidosis in Chronic Hepatitis The Ultrastructures of Pig Kidney Proximal Tubule

肾小管坏死数量较少；猪肾近端小管的超微结构

Necrosis of renal tubule was lesser ; The Ultrastructures of Pig Kidney Proximal Tubule

庆大霉素致大鼠肾近端小管上皮细胞凋亡的超微结构研究

Ultramicrostructure Study on the Apoptosis of Nephrotic Proximal Tubule Epithelial Cells in Rats Induced by Gentamicin

肾小管尿极的壁层上皮与近端小管上皮之间有一明显过渡区。

There were a transitional area between the cells of visceral layer and the proximal tubule .