转染

提高精子转染外源DNA效率的山羊精液冷冻方法

Improvement of the Efficiency of Gene Transfection in Frozen-Thawing Goat Sperm

DNA疫苗的转染载体及免疫途径

Transfection vehicles and inoculation methods of DNA vaccine

B（7-1）基因转染后对小鼠体内的免疫增强作用

Effect of b_ ( 7-1 ) gene on mouse cervical cancer cell immunity in vivo

脂质体介导外源性DNA阳性转染率可高达30%～40%以上。

Transfected rate of exogenous DNA mediated by lipofectin was above 30 % ~ 40 % .

低质量的DNA与细胞过长时间接触降低阳离子性脂质体法的转染效率。

Poor quality of DNA and longer incubating time were associated with decreased transfection efficiency of lipofectin .

SA脂质体介导DNA转染昆虫细胞的研究

DNA Transfection of Insect Cells Efficiently Mediated by Stearylamine Liposome

HBV全基因组克隆转染细胞系的建立

A cell culture system transfected with full-length DNA clone of hepatitis B virus

分别用细胞和夹心酶联免疫吸附试验（ELISA）检测DNA转染细胞膜表面抗原。

The membrane surface antigens of DNA-transfected cells were detected with cellular ELISA and sandwich ELISA respectively .

结果：人骨形态发生蛋白质7基因存在于转染后的骨髓基质干细胞中并表达相应的mRNA。

RESULTS : hBMP-7 gene was contained in MSCs after transfection and expressed the relevant mRNA .

HCV核心基因转染的胆管癌细胞中p53的表达及意义

Expression of p53 in HCV core gene transfected cholangiocarcinoma and its significance

转染细胞的反转录PCR可见E2和GM-CSF两个基因的转录。

Transcription of both E2 and mGM-CSF was detected by RT-PCR .

RNA点杂交检测转染细胞有IL-2R基因mRNA转录产物表达。

IL-2R mRNA expression of transfected cells was assayed with Northern blot .

SA脂质体介导报告基因转染耳蜗的实验研究

Experimental study of transfection of cochlea by SA liposome mediated reporter gene

心房肽cDNA转染细胞微囊化及心房肽近日节律性表达

Encapsulation of Atrial Natriuretic Peptide ( ANP ) cDNA Transfection Cells and ANP Circadian Expression

MTT法检测加入反义寡核苷酸后对转染的包装细胞生长的影响。

MTT was used to detect the cell growth of the packaging cells .

然后通过电穿孔法将此重组RNA转染BHK21/13细胞。

Then the BHK 21 / 13 cells in vitro was transfected with synthesized RNA by electroporation .

荧光原位杂交（FluorescenceinSituHybridization，FISH）实验观察转染质粒在哺乳动物细胞中的存在状态。

Fluorescence in situ hybridization experiment ( FISH ) was observed the status of transfected plasmid in mammalian cells .

腹腔内注射TNF基因转染的LAK细胞对于腹水型肝癌小鼠的疗效

Therapeutic Effect of TNF Gene-transfected LAK Cells on the Ascitic Liver Carcinoma-bearing Mice

经PEG表面修饰的纳米粒仍保持纳米粒的体外转染活性。连接半乳糖基牛血清白蛋白的纳米粒转染活性却反而略有下降。

After the particles were modified with PEG , their transfection activity was still remained .

RI基因在人脐血干细胞中的转染和表达

Transfection and Expression of RI Gene on Human Umbilical Blood Stem Cells

胆管癌抗原肽对转染全长野生型p53的树突状细胞免疫功能的影响

Effects of bile duct cancer peptide on immune response of dendritic cells transfected with full-length wild type P53

LIF基因转染的ES细胞生长与分化特性的研究

Study on growth and differentiation of ES cells transfected with LIF gene

经腹腔转染iNOS基因对大鼠的降血压作用

Effect of iNOS gene transfection via enterocoelia on blood pressure in rats

脂质体转染survivin反义寡核苷酸增加宫颈癌细胞对放、化疗敏感性的实验研究

Survivin Antisense Oligonucleotide Transfection by Liposome Sensitizes Cervical Cancer Cells to Chemotherapy and Radiotherapy

RT-PCR产物电泳结果显示，与转染前细胞、空质粒转染细胞相比，转染survivin反义核酸的细胞SURVIVINMRNA水平明显降低。

As compared to controls , the level of survivin mRNA expression in transfected cells decreased significantly .

MHCⅡ类基因转染对肿瘤免疫的影响

Effects of MHC class ⅱ gene transfection on tumor immunogenicity

阳离子多聚物jetPEI~（TM）对原代培养的猪血管平滑肌细胞的转染效率研究

Study of transfection efficiency of cationic polymer PEI to primary culture vascular smooth muscle cells of porcine

癌胚抗原重组痘苗病毒转染树突状细胞诱导CEA特异性T细胞免疫

Induction of CEA-Specific cytotoxic T lymphocytes by dendritic cells transfected with CEA-recombinant vaccinia virus

微囊化人类心房肽cDNA重组质粒转染细胞对实验性高血压大鼠肾组织学改变的影响

Effects of Encapsulated Plasmid Recombining with hANP cDNA Transfected Cells on Morphological and Histological Characteristic of Experimental Hypertensive Rats

胰岛FasL基因转染对大鼠胰岛移植的影响

The effect of FasL gene transfer to islet cells on pancreatic islet allografts