转化细胞系

犬2型腺病毒E1转化细胞系的特性研究

The studies on CAV-2 sy strain E1 transformed cell line

同时，转化细胞系DNA、RNA和蛋白质出现最大合成速度的时间均相应地延迟。

Meanwhile , the appearance of the maximum synthesis rate for DNA , RNA and protein in transformed cell line exhibited was postponed compared with control cell line .

以苜蓿转化细胞系和对照细胞系为材料，利用同位素标志方法研究了3种大分子物质&DNA、RNA和蛋白质的合成动态。

The synthesis of three kinds of macromolecular substances-DNA , RNA and protein in transformed and control cell line of Alfalfa was studied using isotope-tracing methods .

反义PARP基因真核表达重组体的构建及其HeLa转化细胞系的建立

Construction of antisense PARP cDNA recombinants and establishment of the HeLa transformant after antisense plasmid introducing

对转化细胞系采用Southernblot、NorthernBlot及westernblot杂交分析，证实有稳定的外源基因的整合及表达，而内源性TGFα的表达及合成均

The integration of retrovirus vector and expression of exogenous gene in the transformant PC-7 cells were confirmed by Southern blot , Northern blot and Western blot hybridizations . The gene expression and protein

在一些癌基因的转化细胞系和几乎所有的动物肿瘤中均有ODC基因产物的异常升高。

Elevated levels of ODC gene products are consistantly detected in some oncogene transformed cell lines and virtually all animal tumors .

这些带有GFP标记的转化细胞系为柑橘的有关基础研究提供了良好的试材，目前正用于柑橘体细胞杂交和不同倍性愈伤组织的社会化控制现象与机理等研究。

These transgenic cell lines are valuable materials in citrus basic research and currently being used in studies on citrus somatic fusion and social control mechanism between calluses of different ploidy levels .

目的研究EZH2蛋白在食管上皮永生化细胞系（SHEE）和恶性转化细胞系（SHEEC）中的表达。

Objective To investigate the expressions of EZH2 protein in the human immortalized esophageal epithelial cell line ( SHEE ) and the malignant transformation cell line ( SHEEC ) .

苜蓿转化细胞系核酸和蛋白质合成的研究

Study on the Synthesis of Nucleic Acid And Protein in Transformed Cell Line of Alfalfa

方法以CMV/LacZ质粒转化COS7细胞系，48小时后用XGal染色。

Method CMV / LacZ was transfected into COS 7 cell line by electroporation and X gal staining was Performed 48 hours later .

Survivin是一种凋亡抑制蛋白，在发育的胚胎和人类恶性肿瘤，转化的细胞系中表达，而在终末分化成熟的组织中基本不表达。

The survivin is a number of inhibitor of apoptosis gene family , expressed in embryonic development , human cancer , and transformed cell lines , and unexpressed in terminally differentiated adult tissue .

单纯疱疹病毒转化的细胞系生物学特性的研究

Study of Biological Properties of Cell Lines Transformed by Herpes Simplex Virus

CD96分子表达于正常T细胞、T细胞克隆以及某些转化的T细胞系。

CD96 is expressed on normal T cell lines and clones , and some transformed T cells .

对转化瘤组织细胞系与正常愈伤组织细胞系的生理生化分析表明，两者可溶性蛋白质含量没有明显差异，但转化系过氧化物酶活性和L-苯丙氨酸解氨酶活性却比正常愈伤组织细胞系高得多。

Physiological and biochemical analysis on transformed tumor tissue and callus tissue shows that there aren 't obvious different soluble protein contents between them .

而HNG－E则为发生转化的神经干细胞系，但能在RA诱导下向多巴胺能神经元分化。

HNG-E cells were characterized as transformed neural stem cells , and can be differentiated into dopaminergic neurons by RA induction .

SV40转化人角朊细胞系的生物学特征及其创面移植的实验研究

Experimental Study on Biological Characteristics and Wound Grafting of SV40 Transformed Human Keratinocytes

目的：建立α粒子诱发人支气管上皮细胞（BEP2D）恶性转化细胞的克隆细胞系，研究其核型和DNA链断裂修复能力。

Purpose : To subclone the malignant transformants of human bronchial epithelial cell line ( BEP2D ) induced by α - particles and to analyse their karyotypes and capacity of rejoining DNA double-strand breaks ( DSB ) .

目的证实卡波济肉瘤相关疱疹病毒（KSHV）K12基因在体外可以转化小鼠成纤维细胞系NIH3T3细胞，体内可以诱导肿瘤的形成。

Objective To determine whether the K12 gene of Kaposi ′ s sarcoma-associated herpesvirus ( KSHV ) can transform the mouse fibrosis cell lines NIH3T3 in vitro and to induce tumorigenesis in vivo .

目的研究叶绿酸对反式7,8二羟9,10环氧苯并（a）芘（反式BPDE）恶性转化人支气管上皮细胞系（16HBE）体外生长及细胞周期影响。

Objective To study the effects of chlorophyllin on proliferation and cell cycle in vitro in human bronchial epithelial cell line ( 16HBE ) transformed by trans-benzo ( a ) pyrene-trans-7,8 - dihydrodiol-9,10-epoxide ( trans-BPDE ) .

植物组织培养产生的胚性细胞是遗传转化的良好受体，转化的胚性细胞系再生植株频率高，通过筛选可避免转化嵌合体，获得真正的转基因植株。

Embryonic cell generated by the plant tissue culture is a good receptor of the genetic transformation , regeneration frequencies of the transformation embryonic cell is very high , chimerical plasmid will be avoided by screening , the genuine transgene plant will be obtained .