葡萄糖激酶

目的：探讨GDM的危险因素和GDM患者的产科结果、葡萄糖激酶（GCK）基因与细胞毒性T淋巴细胞抗原（CTLA-4）基因与妊娠糖尿病（GDM）的关系。

Object : To evaluate the roles of GCK gene and CTLA-4 gene in the pathogenesis of gestational diabetes mellitus ( GDM ), determine the risk factors and the obstetric complications of GDM .

中国迟发型2型糖尿病患者葡萄糖激酶基因突变研究

Mutation of GCK gene of Chinese patients with late-onset Type 2 diabetes

PCR-RFLP法检测葡萄糖激酶基因启动子-30位点变异的实验条件研究

The Study of Test Condition in the Variation at 30-Position of the Glucokinase Gene Promoter by PCR-RFLP

酒精对NIT-1细胞葡萄糖激酶基因表达的影响

Effect of ethanol on glucokinase mRNA expression in NIT-1 insulinoma cells

目的探讨小檗碱对胰岛素分泌缺陷型糖尿病大鼠肝脏葡萄糖激酶（GK）相关糖代谢的影响。

Objective To study the effect of berberine on glucose metabolism in rats with insulin-secretion deficiency .

方法收集10个NIDDM家系的112例基因组（DNA），经多聚合酶链反应（PCR）扩增葡萄糖激酶基因第2～10号外显子，SSCP电泳，硝酸银染色筛查点突变。

Methods 112 samples of genome DNA from 10 NIDDM pedigrees were collected . Exon 2 10 of glucokinase gene were amplified by PCR and screened with SSCP electrophoresis and silver staining .

目的建立银染单链构象多态性（SSCP）技术筛查葡萄糖激酶基因点突变。

Objective To establish non isotope single strand conformation polymorphism ( SSCP ) technique of silver staining to screen point mutation of glucokinase gene .

目的了解葡萄糖激酶（glucokinase）基因GCK突变和序列变异在中国人早发性糖尿病人群中的发生情况。

Objective To investigate the prevalence of mutations and sequence variations of glucokinase gene GCK in Chinese early-onset diabetes population .

目的探讨葡萄糖激酶（GCK）基因与妊娠糖尿病（GDM）易感性的关系。

Objective To evaluate the role of glucokinase ( GCK ) gene in the pathogenesis of gestational diabetes mellitus ( GDM ) in Chinese .

目的观察酒精对NIT-1细胞葡萄糖刺激后胰岛素分泌功能及葡萄糖激酶（Glucokinase）基因表达的影响。

Objective To study the effects of ethanol on glucose-stimulated insulin secretion ( GSIS ) and glucokinase mRNA expression of NIT-1 insulinoma cells .

Lb-PS还能降低培养基中NO和MDA的浓度。因此，Lb-PS可能通过减少胰岛β细胞的NO产量和维持SOD和葡萄糖激酶的活性，最终起到保护胰岛素合成和释放功能的作用。

Therefore Lb-PS could protect glucose-induced insulin synthesis and insulin secretion through reducing NO production and maintaining glucokinase and SOD activity in pancreatic β cells .

目的：通过比较肝脏特异性葡萄糖激酶基因敲除小鼠和两种链脲菌素诱导的糖尿病小鼠模型血清肿瘤坏死因子α（TNFα）水平，初步探讨TNFα在糖尿病发病过程中的作用。

AIM : To compare serum tumor necrosis factor α( TNF α) concentration in liver specific glucokinase gene knockout mice and two kinds of streptozotocin induced diabetic mice , and to explore the possible role of TNF α in the pathogenesis of diabetes mellitus .

葡萄糖激酶（Glucokinase，GK）是糖代谢的限速酶，作为葡萄糖的感受器在维持体内血糖稳定中起关键作用。

Glucokinase ( GK ), a rate-limiting enzyme in glucose metabolism , plays a key role in the maintaining glucose homeostasis as a glucose sensor in many vertebrates including humans .

基于此，我们借助相应的分子生物学理论和生物工程技术构建了人源肝脏葡萄糖激酶的表达质粒，并建立了基于大肠杆菌体系的GK高效表达系统。

In this work , we constructed a human liver glucokinase expression plasmid based on corresponding molecular biology theories and bioengineering techniques , and set up a high-efficiency expression system in E.coli .

目的：探讨中国迟发型2型糖尿病患者葡萄糖激酶（glucokinase，GCK）基因的突变情况。

Objective To determine the mutation of the Glucokinase ( GCK ) gene in Chinese patients with Type 2 diabetes . Methods Forty-seven families with Type 2 diabetes were recruited .

应用聚合酶链锁反应扩增葡萄糖激酶（GCK）基因微卫星多态标志，GCK-5'，进行GCK基因与国人非胰岛素依赖型（Ⅱ型）糖尿病关联研究，并与非塘尿病组比较。

The association of glucokinase ( GCK ) gene with type 2 diabetes in Chinese was studied by using the polymerase-chain-reaction amplified microsatellite polymorphism marker , GCK-5 ' .

这样，它对于GKRP的作用是一种补充机制，GKRP也调节葡萄糖激酶蛋白的表达和区间作用。

Thus , it acts in a complementary mechanism to GKRP , which also regulates glucokinase protein expression and compartmentation .

目的检测老年人2型糖尿病（DM2）患者葡萄糖激酶（GCK）等位基因频率的变化，进而从分子水平探讨DM及其血管并发症的发病机制。

Objective To investigate the variation of glucokinase ( GCK ) allele frequency in aged patients with type 2 diabetes mellitus ( DM2 ) and to provide a method for early detection of diabetes mellitus ( DM ) .

对30例起病于45岁或以前及／或伴糖尿病家族史的中国人常见型非胰岛素依赖型糖尿病（NIDDM）患者，进行葡萄糖激酶基因（GCK）编码区及拼接区分子扫查。

The molecular scanning of the coding and junction regions of glucokinase gene ( GCK ) was performed in 30 Chinese subjects of common form non-insulin-dependent diabetes mellitus ( NIDDM ) with age of onset at or before 45 years , and / or with positive family history of diabetes .

葡萄糖激酶及其小分子活化剂研究进展

Advances in the study of glucokinase and small molecule glucokinase activators

关键词：胰岛素抵抗，葡萄糖-6-磷酸酶，葡萄糖激酶，基因

KEY WORDS : insulin resistance , glucose-6-phosphatase , glucokinase , gene expression

逆转录病毒载体介导胰岛素原基因突变体与葡萄糖激酶基因共转染的应用价值

Glucokinase gene and mutant proinsulin gene transfected by retroviral vector

以人胰葡萄糖激酶为靶的降糖活性因子筛选平台的建立

Establishment of a Hypoglycemic Agents Screening Method Based on Human Pancreatic Glucokinase

茶多糖对四氧嘧啶高血糖小鼠有显著的降血糖作用，肝葡萄糖激酶活性增加。

The activity of liver glucokinase was higher than those without tea polysaccharides .

非同位素银染单链构象多态性技术筛查葡萄糖激酶基因突变

Non isotope SSCP technique in screening glucokinase gene mutation

常见型非胰岛素依赖型糖尿病患者的葡萄糖激酶基因分子扫查

Molecular scanning of glucokinase gene in the common form of NIDDM in Chinese

葡萄糖激酶基因多态性与妊娠期糖尿病遗传易感性的关系

Relationship between the polymorphism of glucokinase gene and genetic susceptibility of gestational diabetes mellitus

家族性非胰岛素依赖型糖尿病的葡萄糖激酶基因研究

A study of glucokinase gene in familial NIDDM

实验结束后取肝脏测定糖原含量和葡萄糖激酶活性。

The content of liver glycogen and the activity of liver glucokinase were studied .

中国人早发及多发糖尿病家系中筛查葡萄糖激酶基因突变

Screening of glucokinase gene mutation in early-onset and / or multiplex Chinese diabetes pedigrees