分支酶

玉米淀粉分支酶SBEⅡb基因RNA干涉表达载体的构建和转化

Construction and Transformation of Maize Starch Branching Enzyme SBE ⅱ b Gene RNA Interference Expression Vector

玉米淀粉分支酶sbeⅡb基因启动子的克隆和表达载体构建

Cloning of the Promoter of Maize Starch Branching Enzyme Sbe ⅱ b and Constructing of Plant Expression Vector

PCR和Southernblot杂交检测表明：淀粉分支酶基因正、反向表达结构和可溶性淀粉合成酶正向表达结构均已整合进水稻的基因组中。

PCR and Southern blot showed that soluble starch synthase gene and starch branching enzyme gene have been integrated into rice genome and expressed .

利用农杆菌（Agrobacteriumtumefaciens）介导将淀粉分支酶基因RNA干涉表达载体转入玉米自交系中，并对农杆菌转化系统的条件进行研究。

RNA interference expression vector of starch branch enzyme gene was transformed into maize inbred lines by Agrobacterium tumefaciens and the conditions of Agrobacterium tumefaciens system were studied .

小麦籽粒淀粉分支酶同种型SBEIIb的亚细胞定位及遗传多样性

Subcellular Localization and Genetic Polymorphism of Isoform of Starch Branching Enzyme ( SBE IIb ) in Wheat Grain

淀粉的合成在最后阶段涉及到3个关键性的酶是：ADPG焦磷酸化酶、淀粉合成酶以及淀粉分支酶。它们分别催化ADP－葡萄糖的形成、葡聚糖链的延伸以及分支链的形成。

The last three committed steps in the pathway of starch biosynthesis are catalyzed by three enzymes , ADPG phosphorylase ( AGPP ), starch synthase and starch branching enzymes .

蚊抗药性相关新基因&糖原分支酶基因（NYD-GBE）的克隆和初步鉴定

Cloning , Sequence Analysis , Construction of Expression Plasmids and Functional Identification of Deltamethrin-resistance Associated Glycogen Branching Enzyme Gene ( NYD-GBE ) from Culex Pipiens Pallens

颗粒结合淀粉合成酶（GBSS），淀粉分支酶1（SBE1）和淀粉分支酶3（SBE3）是水稻胚乳中淀粉合成所涉及到的三个最主要的酶。

The granule-bound starch synthase ( GBSS ), starch branching enzymes 1 ( SBE1 ) and 3 ( SBE3 ) are three major enzymes involved in starch biosynthesis in rice endosperm .

在籽粒灌浆过程中可溶性淀粉合成酶、淀粉分支酶和ADPG焦磷酸化酶等3种关键酶活性在水分胁迫下都不同程度的被抑制。

It suggested that water stress inhibited grain filling and delayed active grain filling . Activity of key enzymes in rice grain including soluble starch synthase ( SSS ), ADP-glucose pyrophorylase ( ADPGPPase ) and soluble starch branch enzyme were decreased under water stress . 8 .

水稻籽粒淀粉分支酶活性的遗传分析

Genetic Analysis of Starch Branching Enzyme Activity in Rice Grain

淀粉分支酶和去分支酶编码基因的功能

Functions of Genes Encoding Starch Branch Enzyme and Debranch Enzyme

玉米淀粉分支酶基因表达调控的研究

Research on Expression Regulation and Management of Maize Starch Branch Enzyme Genes

植物支链淀粉合成的关键酶&淀粉分支酶

Starch Branching Enzyme : The Key Enzyme of Amylopectin Biosynthesis in Plants

玉米淀粉分支酶基因反义表达载体的构建和功能分析

Construction and Functional Analysis of Antisense Vector of Maize Starch Branching Enzyme Gene

木薯淀粉分支酶基因双价块根特异性反义表达载体的构建

Construction of Antisense Bivalent-expression Vectors Containing Cassava SBE Gene

另外淀粉去分支酶对淀粉最终结构的形成也起到重要作用。

Recent observations suggest that starch-debranching enzymes also play essential role in amylopectin biosynthesis .

对转双反义分支酶基因大米进行了90天喂养试验研究。

The ninety days feeding study of the transgenic rice expressing double antisense branching enzymes gene .

对水稻胚乳淀粉颗粒结合的淀粉分支酶进行了研究。

The starch branching enzyme bound to starch granule in rice endosperm was investigated in the present paper .

聚合双反义分支酶基因对稻米淀粉理化特性的影响

The Effects of Simultaneously Antisense Gene Targeting of Two Branching Enzymes on the Starch Physiochemical Properties in the Endosperm of Transgenic Rice

淀粉分支酶活性变化呈三次曲线的关系，前期活性较高，支链淀粉合成能力强，后期逐渐下降。

Grain with higher Q enzyme activity at early stage had greater amylopectin synthesis ability , which dropped gradually at late stage .

支链淀粉是植物淀粉的主要成分，而淀粉分支酶是其合成的关键酶。

Amylopectin is a major constituent of starch in plants and starch branching enzyme is thought to be a key enzyme of amylopectin biosynthesis .

另外，1,4-α-葡聚糖分支酶和核糖体蛋白46基因在抗性品系中特异表达。

In addition , 2 genes specially expressed in resistant strain were similar respectively to glycogen branching enzyme gene and ribosomal protein 46 gene .

不同类型土壤上种植的不同面筋含量冬小麦品种灌浆期间籽粒中淀粉分支酶活性变化

Changes of Activity of Starch Branching Enzyme in Kernel During Grain Filling Stage in Winter Wheat Cultivars Containing Different Gluten Planting in the Different Soil Types

试验结果表明：不同类型专用小麦的淀粉含量（%）、直/支比例（%）、淀粉积累动态、花后剑叶、籽粒可溶性糖含量、分支酶活性等存在差异。

Starch content , amylose / amylopectin ratio , starch accumulating dynamic , soluble sugar content in flag leaves and grains after anthesis , branch enzyme activities among different varieties for special end uses were significantly different .

很多研究表明，小麦胚乳淀粉的合成至少需要四类酶&腺苷二磷酸葡萄糖焦磷酸化酶、淀粉合成酶、淀粉分支酶和淀粉去分支酶，这四类酶的基因克隆和特性的研究有重要进展。

Many researches aimed primarily at enzyme characterization show that there are at least four enzymes ( including ADP-glucose pyrophosphorylase , starch synthase , starch branching enzyme and starch ( debranching ) enzyme ) involved in starch biosynthesis of wheat endosperm .

淀粉的功能和用途由其结构和特性所决定，葡萄糖焦磷酸化酶、淀粉合成酶、淀粉分支酶和淀粉脱支酶是参与淀粉生物合成的关键酶。

The function and end use of starch relies on its structure and property . According to our current understanding , starch biosynthesis occurs mainly through the action of four classes of enzymes : ADP-Glc pyrophosphorylase , starch synthase , starch-branching enzyme and debranching enzyme .

结果表明，转基因大米对孕鼠体重增长、孕鼠生殖能力、胎鼠发育（外观、内脏及骨骼）均未产生不良影响，即转双反义分支酶基因大米未对试验大鼠产生致畸作用。

The results indicated that no adverse effect were observed on the body weight gain and fecundity of pregnant rats and fetuses growth ( appearance , viscera and bone ), the transgenic rice expressing double antisense branching enzymes gene have no teratogenesis on test rats . 4 .

分析指出，遮光下淀粉合成酶活性的降低与淀粉合成量的下降有关，淀粉分支酶活性的升高是直链淀粉占淀粉总量的比率减少的重要原因。

It was implied that the decline of starch synthesis enzyme activities was relative to the decrease of starch component , and the increase of the activity of starch branching enzyme played an important role in the decrease of ratio of amylose to the total starch under weak light .

作者进而表达了C末端切除38个氨基酸的T/1-336片段，发现预苯酸脱氢酶活性彻底丧失，而其分支酸变位酶和调节结构域的活性却基本保留。

It lost all prephenate dehydrogenase activity when T-protein was expressed with 38 amino acids cut off at its C-terminus , but chorismate mutase and regulatory activity were both retained .

研究表明，大肠杆菌T蛋白由两个独立结构域组成，N端93个氨基酸组成了分支酸变位酶，C端277个氨基酸组成了预苯酸脱氢酶

In a conclusion , E. coli T protein do have independent domains , N terminal 93 amino acids belongs to chorismate mutase and C terminal 277 amino acids belongs to prephenate dehydrogenase