葡聚糖凝胶

比较了4种从土壤中直接抽提的微生物总DNA的纯化方法，实验结果表明1%的琼脂糖凝胶电泳纯化方法及葡聚糖凝胶G200离心层析纯化方法均不能完全纯化从土壤中抽提的微生物总DNA。

Four methods to purify soil microbial total DNA extracted directly from soil were compared . The results showed that purification method with 1 % Sepharose electrophoresis and with Sephadex G-200 centrifuge filtration could not completely purify total DNA directly extracted from soil microbes .

葡聚糖凝胶层析分离微量DNA技术

Separation of trace DNA by chromatography on Sephadex gel

葡聚糖凝胶过滤分离人x精子的研究

Study on separation of human X spermatozoa using Sephadex gel chromatography

用葡聚糖凝胶G-75层析柱制备高纯度细胞色素C

The Preparation of Highly Pure Cytochrome C by Using Sephadex G 75

将含量较高的前二者进一步纯化，乙酸纤维膜电泳和葡聚糖凝胶G－75柱层析的结果表明纯化后的褐藻酸钠、褐藻糖胶是均一的。

Sodium alginate and fucoidin were further purified , the purity of which was demonstrated homogeneously by chromatography on a sephadex G-75 column and acetate cellulose membrane electrophoresis .

采用葡聚糖凝胶G-25分子筛层析及SDS-PAGE对混合肽进行了相对分子质量分布范围的分析。

The range of molecular weight of soybean peptides was analysed by Sephdex G-25 and SDS-PAGE .

采用葡聚糖凝胶G-100柱层析和醋酸纤维素薄膜纸电泳检测DTA的纯度，实验显示：DTA为单一多糖，利用高效液相色谱分析DTA的组成。

Purity detection indicated that DTA was homogeneous by gel filtration chromatography on Sephadex G-100 and cellose acetate pellicle electrophoresis .

葡聚糖凝胶LH-20柱层析法分离人参皂苷Re的研究

Study on Isolation Method of Ginsenoside Re by Sephadex LH - 20

产物通过交联葡聚糖凝胶（Sephadex）G-150凝胶过滤制备获得D-二聚体的粗品。

Products get through Sephadex gel ( Sephadex ) G-150 gel filtration to obtain crude D-dimer .

同时，葡聚糖凝胶（SephadexG-25）柱层析显示水解物较原清蛋白分子量变小。

The gel filtration chromatography ( Sephadex G-25 ) profile showed the major albumin protein was degraded after hydrolysis .

以葡聚糖凝胶G-10系统考察8种注射用头孢菌素中的聚合物

To Detect Polymer Impurities in Cephalosporins for Injection with Sephadex G-10 System

采用硫酸胺分级沉淀法、葡聚糖凝胶Sephadex-25层析脱盐、DEAE-纤维素纯化等方法获得绵羊血清免疫球蛋白G。

Sheep serum Immunoglobulin G ( IgG ) was purified by ammonium sulfate precipitation , Sephadex-25 chromatography and DEAE-Sepharose column chromatography , respectively .

采用葡聚糖凝胶LH-20层析柱料和适当的分离条件，对人参皂苷Re的分离方法进行了改进。

The method for the isolation of ginsenoside Re has been improved by using sephadex LH-20 column chromatography and choosing proper conditions .

血浆儿茶酚胺荧光测定法氧化铝吸附葡聚糖凝胶G-10分离纯化

Method for Determination of Plasma Catecholamine

若将直接抽提的总DNA先经葡聚糖凝胶G200离心层析纯化，再用1%的琼脂糖凝胶电泳纯化，则能取得较好的纯化效果。

However , if the total directly extracted DNA first purify with Sephadex G-200 centrifuge filtration and then purify with 1 % Sepharose electrophoresis , a fairly good purification effect can be obtained .

超过滤和葡聚糖凝胶G200实验，显示了其分子量的范围和分布。

The ultrafiltration and chromatographic experiments on Sephadex G-200 showed the range and the distribution of molecular weight .

通过对GIF在不同交联度葡聚糖凝胶中kd的分析表明，小牛脾脏GIF的分子量约为496～695u左右。

Analysing the kd of GIF in three types of different Dextran gels , the molecular weight of GIF from calf spleen was estimated to be in the range of 500-700 dalton .

葡聚糖凝胶SephadexLH-20柱色谱分离后，经抗菌活性检测发现两个活性峰，其中第2个峰对金黄色葡萄球菌和大肠杆菌的抑菌活性较强。

On the basis of separation of the Sephadex LH-20 , two peaks with antibacterial activity appeared , and the second peak showed the stronger inhibition activity against S.aureus and E.coli .

结合巯基葡聚糖凝胶（SDG）分离富集，可用于测定环境水样、人发及岩石样品中的痕量锡（Ⅳ）。

Combined with sulphydryl dextran gel separation and enrichment , the method has been applied to the determination of trace tin in environmental water , human hair and rock samples with satisfactory results .

采用亲和层析和葡聚糖凝胶层析技术，从人垂体制品中分离，提纯人促卵泡成熟激素（h-FSH），制备了FSH抗血清，建立FSH放射免疫测定法。

Using the technique of affinity chromatography and glucosan gel chromatography the authors have successfully isolated and purfied human follicle-stimulating hormone ( h-FSH ) from human pituitary gland and established the radioimmunoassay of h-FSH .

蓼属植物化学成分多用甲醇、乙醇等溶剂进行提取，分离多用硅胶、葡聚糖凝胶、Rp-18及Rp-8等色谱法，以氯仿-甲醇、氯仿-甲醇-水、甲醇-水作移动相。

And these compounds are commonly extracted by methanol or ethanol , and separated by silica gel column chromatography and reverse phase chromatography .

方法基于葡聚糖凝胶CM-C25对维生素B6的选择性吸附，采用液滴荧光分析方法对维生素B2和B6混合溶液进行检测。

Methods Based on the selective sorption of Sephadex CM-C25 resin for vitamin B6 , a renewable drop spectrofluorimetry was used for simultaneous determination of vitamin B2 and B6 in a mixed solution .

粉尘螨浸液通过葡聚糖凝胶柱层析，测得洗脱液的光密度值有2个峰（SephadexG-50）或3个峰（SephadexG-200）。

It was found that the optical densities ( OD ) of the fractions had 2 peaks ( Sephadex G-50 ) or 3 peaks ( Sephadex G-200 ), when D. farinae extract passed through gel filtration .

GLL通过配位色谱或两步葡聚糖凝胶柱层析方法分离得到两种灵芝肽GLP-Ⅰ和GLP-Ⅱ。

By using ligand exchange chromatography , or a two-step gel chromatogram method respectively , two peptides , GLP - I and GLP - II , were separated from GLL .

首先采用冰醋酸粗提法提取牡蛎蛋白，然后用葡聚糖凝胶（Sephadex-G100）进行柱层析分离，SDS&聚丙烯酰胺凝胶垂直板电泳分离蛋白，Kodak凝胶成像分析仪分析及测定分子量。

Protein was first extracted with acetic acid and was purified by Sephadex G-100 gel filtration chromatography , then the protein was separated by SDS-PAGE electrophoresis , and the molecular weight of the protein with Gel Image Station ( Kodak ) was determined .

方法：7－（香豆素－3－甲酰胺）头孢菌素在NaI或KSCN的存在下与吡啶和β－甲基吡啶反应，产物用大孔吸附树脂及葡聚糖凝胶柱层析分离。

METHOD : 7 - ( coumarin-3-formamido ) - cephalosporin was reacted with pyridine and β - methylpyridine in the presence of NaI or KSCN ; the prodcts were isolated by macroporous resin and sephadex column chromatogrophy .

SephadexG-15葡聚糖凝胶色谱图上出现5个吸收峰，其中高ACE抑制活性组分的分子量在1320～311之间，IC50为0.77mg/mL。

Gel filtration of the hydrolysate on Sephadex G-15 chromatogram yielded five absorbance peaks , the molecular distribution of the fractions with the most potent inhibition activity was located between 1320 and 311 , the IC50 was 0.77mg/mL .

目前常用的方法是用葡聚糖凝胶的方法分离制备EGCG，也有用高速逆流色谱来分离制备的，还有将两种方法结合起来应用，都取得了很好的效果。

The sephadex gels are used in the common method for preparation of EGCG . The high-speed countercurrent chromatography is also adopted to prepare EGCG . In addition , these two methods are combined into the procedure of preparing EGCG . All of them make good results .

抑菌物质在pH2.0处不被沉降；该物质易溶于甲醇中，基本不能被正己烷和乙酸乙酯溶解；葡聚糖凝胶柱层析能够有效分离抑菌物质。

This substance could not be settled in pH2.0 , was soluble in methanol , but hardly in n-hexane and ethyl acetate , and dextran gel column chromatography could separate antibacterial substances in effect .

多糖Ⅰ依次用离子交换层析（DEAE-52）和葡聚糖凝胶（G-75）进行分离、纯化，结果表明分子中带有同一种电荷，硫酸基离子鉴定反应为阳性，并至少含有三个组分；

Fraction ⅰ was further separated and purified by ion-exchange chromatography ( DEAE-52 ) and Sephadex ( G-75 ) and analyzed with Sephadex ( G-100 ) and agar gel electrophoresis . The results show that fraction ⅰ is composed of three components with sulfate .