脊髓组织

结果HE染色镜检发现脊髓组织病理学改变A组明显轻于B组。

Results The histopathologic changes in spinal cord tissues in group B was worse than that in group A.

本文应用生长抑素、5-羟色胺、P物质，胶质酸性蛋白的抗体，通过免疫细胞化学反应，对胚胎脊髓组织移植后的细胞构筑特征进行了观察。

The cytological and topographical features after fetal spinal cord transplantation were analysed using immunocytochemistry of somatostatin , serotonin , substance P and glial fibrillary acidic protein .

B组脊髓组织NO浓度显著高于A、C组（P<0.01）。

The concentration of nitric oxide in spinal cord in group A was significantly different from that in group A or C ( P < 0.01 ) .

测定脊髓组织中IL-8的水平。

The level of IL - 8 was measured .

结论HBO可增强损伤脊髓组织GAP43表达。

Conclusion HBO can increase the level of GAP 43 expression .

结果Caspase3mRNA在VEGF组脊髓组织中的表达下调，在VEGF拮抗组中表达增高。

Results The expression of Caspase-3 mRNA in spinal cord was down-regulation in VEGF group and up-regulation in ANTI-VEGF group .

废多聚甲醛的回收利用方法：多聚甲醛灌流固定的脑和脊髓组织切片上，进行免疫组织化学（ABC法）染色。

METHODS : Immunohistochemistry was performed on the sections of brain and spinal cord of rats perfused by paraformaldehyde .

目的：证实人胚胎脑及脊髓组织是否存在褪黑素（Mel）受体（MR），阐明Mel生物学特性及对神经系统作用的机制。

Objective : To verify whether there exists melatonin ( Mel ) receptor in human embryonic nervous system .

方法：应用丽春红2R-亮绿双重染色法，对正常SD大鼠脊髓组织进行染色。

Methods : The spinal cord sections of the adult Sprague-Dawley rats were detected by using Ponceau 2R-brilliant green double stain method .

正常对照组脊髓组织中仅有少量散在的NF-kB阳性细胞，其它组均比正常对照组多，差异有显著性（P0.05）。

Numbers of NF-kB positive cells were lower in normal control group than other group ( P0.05 ) .

术后6h、24h取A组、B组和C组动物损伤区脊髓组织测定水和Ca2+、Mg2+含量；

The values of Ca 2 + , Mg 2 + of injured spinal cord tissues were measured at 6 , 24 hours after injury .

方法应用免疫组化及免疫电镜技术研究大鼠脊髓组织中NOS的显微及超微结构特征。

Methods The microscopic features and ultrastructure of NOS in the spinal cord tissue of rats were observed by immunohistochemistry and immunoelectronmicroscopy .

结果：HE染色显示脊髓组织病理学改变B、C、D组明显轻于A组，而D组较B、C组轻。

Results In injured spinal cord , the pathological changes of group B , C and D were less obvious than that of group A , while group D was less obvious than group B and C.

结果正常大鼠脊髓组织内有低水平的IGF-1和Bcl-2表达。

Results In the spinal cord of normal control and sham operation groups , weak expression of IGF-1 and Bcl-2 could be detected .

实验组脊髓组织MDA含量明显低于对照组，再灌注后24，J、时更显著。

MDA content of spinal cord in experiment group was significantly lower than that of spinal cord in control group , especially during the first 24 hours of reperfusion .

细胞增殖核抗原及Bcl-6在损伤脊髓组织中的表达意义

Significance of cellular proliferative nuclear antigen and Bcl-6 expression in spinal cord injury

结论通过对各种条件的优化，初步建立了脊髓组织2-DE技术。

Conclusion With optimization of each condition , 2-DE technique for spinal cord tissue has been preliminarily established .

各组均在缺血前、缺血10min和再灌注1h时对所有动物抽动脉血并取脊髓组织匀浆后分别测定MDA含量和SOD活性。

The MDA and SOD were measured at the 3 different time points : pre-ischemia , 10 min after ischemia and 1 h after reperfusion .

结果：复方丹参处理后脊髓组织MDA浓度明显低于各时相点对照组，SOD活性显著升高（P<0.01），与MP治疗组无明显差异（P>0.05）；

Results : Activity of SOD was elevated , the level of MDA and the impared microcirculation were alleviated in the mammals treated with SM ( P < 0 01 ) .

目的观察兔缺血再灌注脊髓组织中白细胞介素-8（IL-8）的表达，探讨其对脊髓缺血再灌注损伤的影响。

Objective To observe and understand the expression and effect of interleukin-8 ( IL-8 ) in spinal cord during the ischemia / reperfusion ( I / R ) .

结果CCI可导致机械痛阈和热痛阈降低，脊髓组织IL-6mRNA和IL-6表达增加；

Results CCI significantly decreased the mechanical and thermal pain threshold and increased the expression of IL-6 mRNA and IL-6 protein in the ipsilateral spinal dorsal horn .

原位杂交检测SCI前后脊髓组织中ET-1mRNA。图像分析半定量测定伤区邻近段ET-1mRNA含量。

ET-1 mRNA in the spinal cords before and after injury was examined by in situ hybridization and the content of ET-1 mRNA was determined by semi-quantitative image pattern analysis .

损伤后1d肉眼可见脊髓组织肿胀明显，软脊膜紧绷。

At one day after the injury , swelling could be obviously seen in myeloid tissue by nude eyes and the spinal pia mater was in tension .

进行GAP-43、NF200免疫组织化学标记等，观察GAP-43、NF200在上述各组脊髓组织中的表达。

GAP-43 and NF-200 expression were detected by immunohistochemistry labeling in each group after injury .

目的探讨大鼠周围神经损伤后相应神经与脊髓组织髓鞘碱性蛋白（MyelinBasicProtein，MBP）含量变化及神经生长因子（NGF）的影响。

Objective To investigate the effect of nerve growth factor ( NGF ) on the content changes of myelin basic protein ( MBP ) in lumber spinal cord and injured sciatic nerve of Wistar rats .

坐骨神经损伤后不同年龄大鼠脊髓组织中bcl-xl基因的表达及变化

Expression and change of B-cell lymphoma / leukemia-xl gene in spinal cord tissues of rats at different ages after injury of sciatic nerve

但在伤后72h脊髓组织TNF-α水平仍然是对照组组的4.3倍。

Although it decreased after SCI 24h , the level of TNF - in the cord was 4.3 fold against control group at SCI 72h .

用丙二醛试剂盒测定各组细胞上清中丙二醛（MDA）浓度；用SOD、丙二醛试剂盒测试脊髓组织SOD活性和丙二醛的含量。

The supernatant malondialdehyde ( MDA ) contents were assayed by MDA kit ; The activity of SOD and the level of MDA in the spinal cord tissue were measured with SOD and MDA test kits respectively .

应用免疫组化方法观察压迫脊髓组织Nogo-A、萎缩腓肠肌Myf-5表达的变化。

Application immunohistochemical method observation oppression spinal cord tissues Nogo-A , atrophy Myf-5 expression gastrocnemius changes .

结论伤后脊髓组织中ET－1含量增多的主要原因之一是由于其转录水平的异常表达，ET－1是脊髓继发性损伤的重要损害因子。

Conclusions An increase of ET 1 concentration in injured spinal cord tissues is mainly from its abnormal expression of transcription level . ET 1 is a key injury factor in secondary injury after acute spinal cord injury .