肽核酸

脱氧寡核苷酸或肽核酸可与基因双链DNA内的特定序列结合形成三链DNA，三链DNA结构的形成可以抑制靶基因的转录。

Oligonucleotides or peptide nucleic acid can form triplex-helical DNA by binding to specific sequences in double-stranded DNA of gene .

肽核酸（PNA）是以中性酰胺键为骨架的一种DNA类似物。

Peptide nucleic acid ( PNA ) is a DNA mimic that has a polyamide backbone .

肽核酸生物传感器直接检测临床标本中提取的病毒基因组DNA

Direct Detection of Genomic DNA of Virus from Clinical Samples with PNA Biosensor

缓冲液pH值对肽核酸压电基因传感器阵列杂交效应的影响

Effects of pH values of buffer on hybridization efficiency with PNA piezoelectric gene sensor array

目的以肽核酸生物传感器检测系统直接检测从临床标本中提取的乙型肝炎病毒基因组DNA。

OBJECTIVE To directly detect HBV genomic DNA extracted from clinical samples with peptide nucleic acid ( PNA ) biosensor .

应用肽核酸钳制PCR技术检测胰腺癌K-ras基因突变

Peptide nucleic acid-mediated one-step PCR assay in detection of K-ras mutation

结论利用肽核酸生物传感器成功地绕过了PCR扩增而直接检测出了临床标本中的HBV基因组DNA。

CONCLUSIONS HBV DNA extracted from clinical samples were directly detected using PNA biosensor and PCR amplification was successfully bypassed .

漏声表面波&双体肽核酸生物传感器的构建及检测HPV的实验研究

Construction of Leaky Surface Acoustic Wave Bis-PNA Biosensor and the Detection of HPV

肽核酸（PNA）是DNA模拟物，能与DNA和RNA以序列特异性方式结合，在基因研究和基因治疗方面有广泛的应用前景。

Peptide nucleic acids ( PNA ) are DNA mimics that bind strongly to DNA and RNA in a sequence specific manner .

肽核酸作为DNA的类似物，在与相应的DNA或RNA序列杂交反应时，表现出更高的特异性和更好的稳定性。

PNA ( Peptide nucleic acid ), as analogs of DNA demonstrates a higher specificity and stability in the hybridization reaction with the corresponding DNA / RNA sequences .

然而，与大多数DNA和RNA寡核苷酸不同，肽核酸的透膜能力很差，部分原因是由于它们具有不带电荷的性质。

However , unlike most of the DNA and RNA oligonucleotides , PNAs are poorly penetrated through the cell membrane , partially due to their uncharged property .

为了优化肽核酸的性质，各种结构的肽核酸（PNA）单体被合成出来。

For optimizing the characteristics , various PNA mono - mers had been synthesized .

前言：肽核酸（PNAs）是一个在DNA杂交和反义技术领域迅速崛起的强大工具。

Introduction : Peptide nucleic acids ( PNAs ) are rapidly emerging as powerful tools in DNA hybridization and antisense techniques .

超声波辅助转染PDGF-bmRNA的反义寡肽核酸抑制兔动脉再狭窄

Ultrasound promotes the delivery of an antisense oligo - peptide nucleic acid targeting against PDGF-B mRNA to inhibit the vascular restenosis

反义Ki-67肽核酸对裸鼠人肾癌细胞移植瘤的治疗作用

The Effects of Anti-sense Peptide Nucleic Acids Targeting Ki-67 mRNA on Implanted Human Renal Cell Carcinoma in Nude Mice

目的探索乙型肝炎病毒（HBV）靶序列浓度值对肽核酸压电基因传感器阵列杂交效应的影响。

OBJECTIVE To investigate the effects of hepatitis B virus ( HBV ) target concentration on hybridization efficiency of peptide nucleic acid ( PNA ) piezoelectric gene sensor array .

肽核酸和RecA蛋白&互补单链DNA探针生物信号放大系统用于压电基因传感器的实验研究

Experimental Study of Piezoelectric Gene Sensor by PNA and the System of Biology Magnified of " RecA Protein-complementary Single Strand DNA " Probe

反义肽核酸抑制人肾癌细胞系Ki-67基因的研究

Suppression of antisense peptide nuclear acids on the growth and expression of Ki-67 gene in human renal carcinoma cell line

反义肽核酸对树突状细胞CD86表达的抑制作用

Antisense Inhibition of Gene Expression in Human Dendritic Cells by Peptide Nucleic Acid Against CD86

反义肽核酸是人工合成的DNA类似物，与核酸分子比较，具有较高的水溶性、稳定性和碱基特异性，容易被细胞吸收。

Antisense peptide nucleic acid ( asPNA ) is one of the synthetic DNA analogs with high solubility in water , high stability and binding specificity compared with nucleic acid molecule , which is easily absorbed by cells .

使用肽核酸（PNA）做为芯片探针有望解决上述的问题。

Using peptide nucleic acid ( PNA ) as the probe of microarray is expected to solve the above problem .

目的探索不同肽核酸（PNA）探针浓度对PNA石英谐振式基因传感器阵列杂交效应的影响。

Objective To investigate the effects of peptide nucleic acid ( PNA ) probe on hybridization efficiency with PNA quartz oscillation gene sensor array .

目的探讨反义端粒酶肽核酸（PNA）片段对肺癌细胞株端粒酶活性及细胞株生长的抑制作用。

Objective To determine the inhibitory effect of antisense peptide nucleic acids ( PNA ) of telomerase on the growth of lung cancer cell lines .

肽核酸（PNA）是具有类多肽骨架的DNA类似物，PNA的主链骨架是由N（2-氨基乙基）-甘氨酸与核酸碱基通过亚甲基羰基连接而成的。

Peptide Nucleic Acids ( PNA ) are DNA analogues in which the naturally occurring nucleobases are attached via methylene carbonyl linkages to an achiral pseudopeptide backbone of N - ( 2-aminoethyl ) glycine units .

在信号的放大有同位素、生物素或DIG标记的cDNA和cRNA探针，分支探针和肽核酸探针；

The cDNA and cRNA probe which labeled with the isotope % biotin or DIG , the offset probe and the peptide probe can be applied to magnify the signal .

目的在构建好的肽核酸压电基因传感器检测系统上引入RecA蛋白互补单链DNA探针复合体作为新型生物信号放大系统，提高传感器的检测灵敏度。

Objective To improve the detection sensitivity of peptide nucleic acid ( PNA ) piezoelectric gene sensor by introducing RecA protein-complementary single strand DNA probe complex as new style biological signal amplification system .

目的探讨Ki-67基因翻译起始区反义肽核酸对人肾癌细胞Ki-67基因表达及生长的影响。

Objective To investigate the influence of antisense peptide nuclear acids of Ki-67 gene translation initiator on the growth of human renal carcinoma cell strain and the Ki-67 protein expression .

肽核酸（Peptidenucleicacid，PNA）是借助计算机的辅助设计出的一种人工合成的DNA的模拟物。

Peptide nucleic acid ( PNA ) is a nucleic acid mimics design by CAD , in which the sugar phosphate backbone of natural nucleic acid is replaced by a synthetic peptide backbone formed by N - ( 2-aminoethyl ) - glycine units .

设计合成了含组氨酸残基碱基为胸腺嘧啶的手性肽核酸单体，咪唑氨基的最终保护基为2,4-二硝基苯基（Dnp）。

A chiral peptide nucleic acid ( PNA ) monomer containing histidine residue and thymine has been designed and synthesized employing 2,4-dinitrofluorobenzene to protect the NH group of imidazole .

肽核酸（PNA）作为一种反义核酸类化合物，在分子水平的疾病诊治上，有其独特的优越性，现在日益受到关注。

PNA , which is one kind of anti sense nucleic acid compounds , has its unique advantages in the molecular diagnostics and treatment of diseases . Now , people gradually attach more importance to PNA .