聚腺苷酸

利用多聚腺苷酸信号缺陷逆转录病毒转化人胃上皮细胞GES-1

Polyadenylation signal - deficient retroviruses transformation of human gastric epithelial GES-1 cells

两个基因序列上均有Kozak序列及加尾信号多聚腺苷酸，证明了克隆序列的完整性。

Both Kozak sequence and polyadenylation signal locating on the sequence revealed the integrity of the sequences we cloned .

基因从核内转录后，新合成的pre-mRNA或核异质RNA（hnRNA）通过加帽、pre-mRNA的剪接、合成多聚腺苷酸（PolyA）尾等一系列的反应而成为成熟的mRNA。

When gene was transcripted from cell nucleus , the new-synthesized pre-mRNA or hnRNA become mature RNA through a series of responses such as add-cap , splice of pre-mRNA , compose poly-A et al . Then the mature mRNA was transport to cytoplasm .

一种快速非同位素测定2'，5'-寡聚腺苷酸合成酶活性的方法

A Rapid Non isotope Assay for 2 ′, 5 ′ Oligoadenylate Synthetase

多聚腺苷酸信号缺陷病毒捕获宿主序列导致细胞转化

Transformation of rat fibroblast cells REF-1 by polyadenylation signal-deficient retroviruses capturing host sequences

突变类型包括错义突变、移码突变和多聚腺苷酸A的缺失，且基因改变者的免疫组化结果与之完全一致。

The gene alterations were completely consistent to the results of immunohistochemistry examination .

方法使用人工定点突变多聚腺苷酸化信号的小鼠逆转录病毒载体，应用PA317病毒包装细胞获得多聚腺苷酸信号缺陷的重组逆转录病毒；

Methods The polyadenylation signal-deficient retrovirus vector mutated by PCR site-directed mutagenesis was used to make polyadenylation signal-deficient retroviruses by PA317 packaging cells .