多核苷酸

固定化多核苷酸磷酸化酶的研究&Ⅱ.用固定化酶制备PolyI和PolyC

Studies on immobilized polynucleotide phosphorylase ⅱ . the application of immobilized enzyme for the preparation of Poly I and poly C

多核苷酸合成的研究Ⅲ.CpUpCpG>p的合成

Studies on polynucleotide synthesis ⅲ . synthesis of cpupcpg > p

1953年Watson和Crick提出了DNA的双螺旋结构模型：DNA分子由两条多核苷酸链组成，这两条核苷酸链通过碱基的互补配对相互缠绕形成一种双螺旋结构；

Watson and Crick advanced the double helix structure model of the DNA in 1953 . The DNA is made up of two pieces of nucleotide chains , which is double helix structure with complementary pairing of the bases and winding each other .

其原理是用多核苷酸磷酸化酶（PNPase），使7-甲基鸟苷5'二磷酸（m~7GDP）在引物存在下聚合，然后在温和的化学条件下，选择性消去7-甲基鸟苷（m~7G）。

Its principle is based on the polymerization of 7-methylguanosine 5 ' diphosphate ( m ~ 7GDP ) on an oligonucleotide primer in the presence of polynucleotide phosphorylase ( PNPase ), followed by selective elimination of m ~ 7G under mild conditions .

非连续多核苷酸定点突变的方法

A Method for Site directed Mutagenesis of Non consecutive and Multiple nucleotides

多核苷酸键是螺旋形的。

A spring is a spiral . The polynucleotide chain is helical .

大肠杆菌多核苷酸磷酸化酶的研究Ⅰ.酶的提纯与性质

Studies on polynucleotide phosphorylase of Escherichia coli ⅰ . purification and properties

多核苷酸的3′-端磷酰化

The phosphorylation of polynucleotide at the 3 ' - end

异染色质大部分是由短而重复的多核苷酸序列所组成的。

Heterochromatin has shown to be composed largely of short repeated polynucleotide sequences .

多核苷酸合成化学新进展

Recent Progress in the Synthetic Chemistry of Polynucleotides

目的：选择多核苷酸磷酸化酶快速离子交换层析的最佳条件。

OBJECTIVE : Selection of optimal chromatographic conditions for purification of polynucleotide phosphorylase in fast ion-exchange chromatography .

提供了新的植物衍生的调控序列和构建体以及使用这种序列指导外源多核苷酸序列在植物中表达的方法。

Novel plant derived regulatory sequences and constructs and methods of using such sequences for directing expression of exogenous polynucleotide sequences in plants are provided .

本发明还涉及所述多核苷酸序列和所述多肽的生产方法，以及这些多核苷酸和所述多肽的用途。

The present invention also relates to the production process of the said polynucleotide sequence and the polypeptides as well as the use of these polynucleotides and the said polypeptide .