紫膜

用园二色（CD）方法相应地观察了温度对紫膜碎片和单体菌紫质分子在可见波长范围内的CD谱的影响同样观察到温度对单体菌紫质分子的CD谱有明显影响。

We also have measured the effect of temperature on CD spectrum of purple membrane fragment and monomeric bacteriorhodopsin , and found that the temperature significantly affects the CD spectrum of the monomeric bacteriorhodopsin .

本文采用荧光探针的方法，通过测量与紫膜结合后的荧光探针ANS的荧光寿命随介质pH的变化，研究了介质pH对bR的构象的影响。

By measuring fluorescent lifetime of AXS binding with purple membrane , the pH - dependence of bacteriorhodopsin conformation was studied .

pH对紫膜表面电位的影响

PH-dependence of surface potential of purple membrane studied by a fluorochrome probe

本实验测定了不同pH条件下嗜盐菌紫膜中细菌视紫红质（bR）的共振拉曼光谱。

Resonance Raman spectra of Bacteriorhodopsin ( bR ) were obtained at different pH.

紫膜（BR）水溶液共振Raman光谱的温度相关性

Temperature dependence of resonance Raman spectra of Br in Agueous solution

报道了在高碱性pH下，紫膜中细菌视紫红质（BR）表面结构变化的直观信息。

The direct information of the surface structure change of purple membrane under high pH was obtained .

紫膜LB膜中的光学相位共轭研究

Optical phase conjugate in Langmuir-Blodgett films of purple membrane

本文用准共振喇曼光谱（RRS）作为研究光动力损伤过程的探针，从分子水平上研究了光敏剂血卟啉衍生物与紫膜菌紫质的作用。

This paper analyzes the photodynamic damage of bR by HPD using resonance Raman Spectroscopy as probe .

紫膜中具有质子泵功能的菌紫质（bR）是整合膜蛋白，它是7个α螺旋跨膜蛋白家族的基本原型。

Bacteriorhodopsin ( bR ) in the purple membrane with the function of proton pumping is an integral membrane protein , and it is a prominent prototype of the family of seven α helical proteins .

研究了紫膜LB膜中的紫膜碎片的结构特性。扫描电子显微镜观察表明，紫膜LB膜中单个紫膜碎片的直径大约为0.3微米。

We have studied the structure properties of purple membrane fragments in purple membrane LB film , scanning electron microscopic observation showed that the diameter of small single purple membrane was about 0.3u .

内源荧光研究发现，在室温下，蓝膜和紫膜中BR蛋白质的最大激发波长都在280nm。

The endogenous fluorescence spectra studies indicate that the maximum excitation wavelengths of bacteriorhodopsin fluorescence in blue and purple membrane at room temperature are both 280 nm .

实验结果表明：CHAPS的增溶过程对紫膜的表层微环境和深层微环境的影响不同。

The results showed that the effects of solubilization by CHAPS on the micro-surroundings of polar surface area in purple membrane bilayer were different from that in hydrophobic inner area .

与此不同的是，随着温度的升高，在紫膜中只能观察到视黄醛的570nm特征峰光吸收的降低。

The characteristic absorption band at 570 nm decreases with the increasing temperature , which is different with blue membrane . 2 .

该菌株在光照条件下，38℃摇瓶培养6d，可用高速冷冻离心、透析袋、蔗糖密度梯度纯化、超速离心等得到紫膜。

The strain was cultured under light treatment , temperature 38 ℃, shake cultivation 6 days , the purple membrane was gained through high speed freeze centrifugation , dialysis , sucrose density gradient centrifugation , superspeed centrifugation etc.

pH3.8，M412的1567cm~（-1）特征振动带作为峰肩出现在变宽了的1536cm~（-1）带上，表明紫膜吸附在金属表面上并不阻断其光化学循环。

This band as a shoulder on the widening 1536cm ~ ( - 1 ) band at pH 3.8 shows that adsorption of purple membrane on the metal surface does not interupt its photochemical cycle .

生物素结合对紫膜结构和功能的影响

The modification effect of biotin on structure and function of purple membrane

嗜菌紫膜表面电位和质子泵效率间的关系

The relation between surface potential and proton pumping efficiency of purple membrane

紫膜的紫外荧光及分子内的能量转移

The ultraviolet fluorescence and intramolecular energy transfer of purple membrane

紫膜表面增强共振拉曼散射谱的研究

Studies on surface enhanced Resonace Raman spectra of purple membrane

文中讨论了紫膜上金属离子结合位点的空间结构特点。

The special conformational property for metal-binding sites on purple membrane was discussed .

酰化对紫膜结构的影响

The effects of acetylation on structure of purple membrane

中性红再生紫膜的光化学性质

The photochemical properties of neutral red regenerated purple membrane

紫膜脂质体的制备及其质子泵功能的检测

Preparation of Phospholipid Vesicles Containing Purple Membrane and Detection of the Proton Pumping Function

有取向紫膜薄层光电位及其与光强度的关系

The photovoltage in the dried oriented purple membrane film and its dependence on light intensity

电场作用下紫膜中细菌视紫红质的圆二色性研究

Studies on circular dichroism of bacteriorhodopsin in purple membrane under the action of electric field

紫膜热转变与水合

Hydration dependence of purple membrane thermal transitions

紫膜与溶剂的相互作用

The interaction of purple membrane with solvents

蜂毒素与紫膜的相互作用机理研究&与三种表面活性剂的比较

The mechanism of interaction of MELITIN with purple membrane ── compared with three kinds of surfactants

冷冻干燥对紫膜菌紫质圆二色性的影响

Effect of freezing and / or drying on circular dichroism of bacteriorhodopsin in purple membrane fragments

这些结果表明，不同价金属离子与紫膜有不同结合方式。

These results suggest that diverse valence metal ions exist in different binding ways from pM .