突变株

经过摇瓶发酵及HPLC检测，该突变株丧失产生阿维菌素的能力。

Shake flask experiment and HPLC analysis showed that the mutant lost the ability to produce avermectins .

金属离子、培养温度、pH、气/液比等也对突变株的生长有一定影响。

Metal ion , temperature , pH , gas / liquid etc. have a great influence upon mutant strain of silicate bacteria .

对高活性突变株M14的细胞转化和发酵转化条件进行优化

The conditions of fermentable and enzymatic conversion of M14 were optimized .

慢性乙型肝炎病毒C区基因突变株对干扰素治疗的应答反应

Core promoter mutations for the response to interferon in patients with chronic hepatitis B

HBV全基因C区突变株在永生化B淋巴母细胞系中的稳定表达

Stable expression of HBV C gene mutants in immortalized human B-cell lines

人胚肺成纤维细胞突变株Z-HL（16）C转化性质的初步鉴定

Preliminary identification of the mutant human embryonic lung fibroblast Z-HL_ ( 16 ) C cell strain

同时利用PCR方法对突变株进行鉴定。

And the mutant was confirmed by PCR method .

利用PCR介导的基因置换技术构建阿维链霉菌bkdF~-突变株

Construction of Streptomyces avermitilis bkdF ~ - Mutants Using PCR-mediated Gene Replacement Method

Southern杂交的结果表明，突变株染色体上只有1个转座子插入位点。

Southern blot hybridization showed that both mutants contained only one insertion site in their chromosomes .

将打靶载体电穿孔入卡介苗，筛选并鉴定卡介苗ERP基因缺失突变株。

BCG mutation with deleted ERP gene was constructed .

通过对突变株的基本特征以及PCR鉴定后，再进行插入基因定位。

Mutants were identified by basic characteristic of Salmonella pullorum , special PCR and gene location .

由于蓝藻基因组含有多个拷贝，经PCR方法检测，证明得到的菌株为单交换的突变株。

Since cyanobacteria contain multiple copies in the genome , the strains obtained are single exchange mutants .

从一些浅黄色突变株引起的病斑上获得的单孢培养菌中，野生菌株（WildType）色素的频率范围为10%～100%。

Lesions derived from some buff mutants given conidial cultures with wild type pigment at frequencies ranging from 10 % to 100 % .

结论太原地区孕妇HBV感染者携带a决定簇突变株。

Conclusion Pregnant women infected by HBV in Taiyuan carried a determinant cluster variant strains .

在HBV感染者体内，突变株往往与野生型病毒一起成为混合病毒群。

HBV mutants often mix with wild-type viruses in HBV carrier and forming a mixed population .

PCR扩增结果表明在白化致死突变株系中存在几丁质酶基因，证明转化成功；

Chitinase gene had been amplified by PCR in albinos lethal mutant line , that certificated transformation was successful ;

在西安地区HBeAg阴性患者中检出乙型肝炎病毒前C区基因突变株

Detection of pre-C region mutants in hepatitis B virus from HBeAg-negative patients in Xi'an area of China

Uγ－1突变株的菌落形态为菊花形，白色。

The colony morphology of U γ - 1 mutant is chrysanthemum type and it is White .

结果筛选到一株遗传性状稳定的高产氢突变株，具有良好的耐酸性，在pH值3.0时仍能生长。

An aciduric mutant with steady hydrogen-producing capability , which was able to grow at acidic pH of 3.0 , was obtained .

RAPD分析进一步证明，突变株和出发菌株存在遗传差异。

RAPD analysis further proved the genetic difference existed between the initial and mutated strains .

高产柠檬酸的SHAM特性黑曲霉突变株的选育

High Producing Citric Acid by Selection of SHAM Mutants of Aspergillus niger

目标产物经HPLC定性分析，确定为反式虾青素。因此，以辛伐他汀等HMG-CoA还原酶抑制剂作为选择压力，对虾青素高产突变株的筛选上具有较好的筛分能力。

The objective product identified by qualitative HPLC analysis was trans-astaxanthin .

PCR检测和测序结果表明已成功地构建了鳗弧菌金属蛋白酶基因突变株。

In conclusion , the construction of metalloprotease gene mutant will surely be beneficial to further study of the pathogenesis of Vibrio anguillarum .

枯草芽孢杆菌突变株CW－06产生β－葡聚糖酶的纯化及其性质的初步研究

Studies on Purification and properties of β - glucanase produced from Bacillus subtilis mutant cw-06

AspergillusOryzae3.951白孢突变株的选育及其特性的研究

Selection and characterization ofa white spore mutant for soy sauce making from Aspergillus oryzae 3.951

为了避免上述问题，本文改造了突变株及回补株构建过程中使用的质粒，使其可以利用Gateway克隆技术进行克隆构建工作。

To avoid the above problems , the plasmids utilized for mutant and complemented strain construction were modified to be Gateway-compatible .

HIV分离株显示明显的变易，特别在包膜抗原&变易性可能促进中和抗性突变株的出现。

HIV isolates show a marked variation , especially in the envelope antigens & variability that probably promotes the emergence of neutralization-resistant mutants .

流式细胞仪测定单克隆抗体识别福氏2a痢疾杆菌及其突变株表面抗原分子表达的分析

Preliminary Analysis of Surface Antigen Expression on Shigella flexneri 2a using Flow Cytometry

感染者体内存在的HCV准种反映在特定环境下以病毒复制、变异为基础的突变株竞争性选择的结果。

Existence of HCV quasispecies reflectes the results of competitive selection based on the viral replication in certain circumstances .

经血清学鉴定，该突变株保持母系菌株的Vi抗原表型。

The Vi antigen phenotype of the mutant did not change in contrast to wild strain with serological method .