磷酸缓冲液

在以前的方法中主要通过使用磷酸缓冲液或甲醇水溶液对蔬菜样品进行简单的表面漂洗，以实现对农药残留的提取。

In past the pesticides residue in vegetables was extracted with PBS or methanol solution by simple rinse .

在240￣300nm吸收峰区间内，乙腈溶液中木瓜蛋白酶的紫外光吸收值比在磷酸缓冲液中的高。

In the absorption peak section of 240 ~ 300 nm , the ultraviolet absorption in acetonitrile solution was higher than that in phosphate buffer solution .

卷烟烟雾磷酸缓冲液中H2O2生成量的测定

Determination of the Amount of Hydrogen Peroxide Generated from Cigarette Smoke in Phosphate Buffer Solution

应用紫外可见吸收光谱法研究细胞色素C（FecytC）活性中心铁离子在pH71的磷酸缓冲液中与4种微量元素M（Ⅱ）的直接相互作用。

The direct interaction between the metal ion in the active center of cytochrome C ( Fecyt-C ) and four trace elements in pH 7.1 phosphate buffer by UV spectrometry has been studied .

最佳反应条件：（1）POD和PPO酶液提取及反应的最佳磷酸缓冲液的pH为6.0；

Optimal reaction condition : The activity of POD and PPO was the strongest when pH of PBS was 6.0 than the others .

将冷冻-解冻后的琼脂包被孵出胚泡分两组，分别置于12%蔗糖液（A）和磷酸缓冲液（B）中进行2分切割。

Hatched blastocysts embedded with agar were frozen , thawed and then bisected in 12 % sucrose in distilled water ( group A ) and phosphate buffered saline ( PBS , group B ) .

结果组织经质量浓度25%蔗糖磷酸缓冲液固定OCT包埋冰冻保存后，从中提取的RNA仍保持较好的完整性，可扩增出较长片段的病毒RNA。

Results The RNA extracted from the frozen OCT-embeded tissue still remained better integrity , it could be amplified to longer segment of viral RNA .

本试验比较了甘氨酸缓冲液、牛肉膏溶液、磷酸缓冲液3种最为常用的洗脱液在不同pH条件下（7、9.5、11）的最佳洗脱效果。

In this study , we compared the best elution efficiency among glycine buffer , beef extract solution and phosphate buffer in different pH ( 7 , 9.5 , and 11 ) .

后者随SO2熏气剂量的增加而增高，并与磷酸缓冲液呈交互促进效应，同工酶谱显著增强。

The latter increased according to SO 2 fumigation and showed relative gain effect with phosphate buffer the isoenzyme pedigree increased markedly .

血浆样品经DTE-磷酸缓冲液提取，过SPE小柱净化浓缩，甲醇洗脱。

Plasma sample was extracted using dithioerythritol-phosphate buffer , and then concentrated and purified by SPE course and eluted with methanol .

结论从25%蔗糖磷酸缓冲液固定OCT包埋的冰冻时间较长的组织标本中可提取组织RNA，并用于进行RT-PCR扩增。

Conclusion RNA can be extracted from the OCT-embedded long-stored tissues treated in 25 % sucrose in PBS . And all the extracted RNA can be used to RT-PCR amplification .

L-1，pH7.4的磷酸缓冲液（PBS）中，37℃恒温振荡，每24h更换一次缓冲液，测定并计算缓冲液中药物浓度及药物累计释放百分率。

The buffer was changed once in every 24 h , and was determinated and calculated drug concentration and cumulative release percent .

将VEGF以微渗透压泵导入实验组豚鼠右耳蜗，对照组导入等量磷酸缓冲液。

VEGF was pumped into the right cochleae of guinea pigs of experimental group by micro-osmotic pump , and the same volume of PBS as that of VEGF was pumped into the right cochleae of control group .

结果表明，通过SDS-磷酸缓冲液可将谷蛋白聚合体分成两部分：分子量较小的SDS-可溶性谷蛋白聚合体和分子量较大的SDS-不溶性谷蛋白聚合体（GMP）；

The results showed that : 1 ) the smaller molecular weight ( SDS soluble ) and the larger molecular weight ( SDS insoluble ) glutenin polymers could be partitioned from glutenin protein by SDS phosphate buffer ;

本文报道了用Radial-PakC18柱，以含有23%乙腈的磷酸缓冲液作流动相，用带有紫外检测器的高效液相色谱测定硫酸多粘菌素E中的多粘菌素E1和E2方法。

The method for determining Polymyxin E_1 and E_2 in Polymyxin E sulfate by High-performance Liquid Chromatography with ultraviolet detector and 23 % acetonitrile in phosphoric acid buffer as mobile phase and Radial-Pak C_ ( 18 ) Column is reported .

分别以二甲基亚砜（DMSO）和磷酸缓冲液（PBS）作为吸收液，用大气收集器（U形多孔玻板吸收管）采集香烟主流烟雾，制得DMSO烟雾溶液和PBS烟雾溶液。

The mainstream whole smoke was collected using atmosphere collector & U shape glass plate absorption tube with poly hole , and both dimethyl sulfoxide ( DMSO ) and phosphate buffer solution ( PBS ) were used as absorbents respectively .

结果表明，当石榴叶（g）与磷酸缓冲液（mL）之比为1∶2，磷酸缓冲液pH值为7.6，提取时间为1.5h。

The results showed that the optimal isolating condition were : the ratio of the pomegranate leaves to the phosphoric acid buffer solution was 1 ∶ 2 ( g / mL ), the phosphoric acid buffer solution pH value was 7.6 , the extractive time was 1.5 h.

在pH7.0的磷酸缓冲液中，用循环伏安法制备了聚甲苯胺蓝修饰碳纤维微柱电极。

Poly-toluidine blue ( PTB ) modified carbon fiber microcylinder electrode ( CFME ) was prepared in phosphate buffer solution ( pH 7.0 ) by cyclic voltammetry .

方法结扎小型猪冠状动脉前降支创建心肌梗死动物模型，分两组，分别于结扎后2周经前降支注入自身骨髓干细胞和磷酸缓冲液（PBS）。

Methods Chronic myocardial infarction models were created in 9 health porcines by left anterior descending artery ligation . The models were divided into 2 groups , and self bone marrow cells or PBS solution were injected via anterior descending artery 2 weeks after ligation , respectively .

结果表明Zamboni氏固定液用于原位杂交组织化学与免疫组织化学结合法最佳，而4%多聚甲醛磷酸缓冲液用单纯原位杂交最佳。

The results showed that Zamboni 's fixative was the best for the combination of in situ hybridization histochemistry with immunohistochemistry while 4 % paraformaldehyde-phosphate buffer was best for in situ hybridization histochemistry .

结果载药壳聚糖胶囊在pH1.2盐酸溶液及pH6.8磷酸缓冲液中药物累积释放量6h内不大于10%，而在模拟大肠液中，4h释药基本完全。

RESULTS Neither of the accumulated release of selected drugs in the capsule was more than 10 % within 6 h in the artificial medium of stomach and small intestine , while the release reached to about 100 % in the case of the large intestinal liquid in 4 h.

然后将其浸入冰冷的40g/L0.1M多聚甲醛磷酸缓冲液中，4℃下固定24小时（PH7.3）。

Tissue including the tooth germs or primordial were dissected from the embryos and fixed in the ice-cold 4 % paraformaldehyde in 0.1M phosphate buffer ( PH 7.3 ) 24h at 4 ℃ .

磷酸缓冲液的防护作用因植物种类而异。

The protective effect of phosphate buffer was different with plant species .

磷酸缓冲液对亚硫酸伤害的防护作用研究

Study on the Protective Function of Phosphate Buffer to Sulphurous Acid harm

采用β-甘油磷酸缓冲液提取食管上皮组织中糖原或含糖物质。

Glycogen from the esophageal mucous epithelium was extracted with β - glycerophosphate buffer .

100例正常血清乳酸脱氢酶同工酶焦磷酸缓冲液琼脂糖电泳法测定与方法学探讨

100 cases normal serum LDH isoenzyme pyrophosphoric buffer agarose gel electrophoresis determination and methodological study

磷酸缓冲液对大豆种子萌发期抗冷性的影响

Effect of Phosphate Buffer on the Chilling Injury Resistance of Glycine Max Seed in the Bud

它在稀磷酸缓冲液，pH6.8,28～30℃结晶。

And crystallized in a dilute phosphate buffer , pH 6.8 , at 28 ~ 30 ℃ .

磷酸缓冲液对小麦苗保护酶系的影响及与SO2污染的关系

Phosphate buffers effect on protective enzyme system and relationship of enzyme system with so_2 pollution in Wheat Seedlings

方法用pH-7.2～7.4的磷酸缓冲液从人毛发中抽提出多肽生殖激素，并用常规的放射免疫方法测定。

Methods Polypeptide hormones were extracted by pH 7.2 ~ 7.4 phosphate buffer solution and assayed by the current RIA of DPC instruction .