瞬时转染

并用RT-PCR和westernblot检测质粒瞬时转染后的靶基因RNA水平和蛋白水平的变化。

RT-PCR and Western Blot were adopted to detect the mRNA and protein levels of NRas and c-Myc after transient transfection of these plasmids .

对于活性较高的化合物，我们还用细胞瞬时转染和MTT实验测试了它们在细胞水平的活性。

Some high-potency molecules were then subjected to transient transfection and MTT assays in order to test the activities of them on cell levels .

体外瞬时转染野生型PTEN过表达对乳腺癌MCF-7细胞的影响

Effects of overexpression of transiently transfected wild PTEN on breast cancer MCF-7 cells

方法采用瞬时转染报告基因测定法，测定细胞裂解液荧光素酶活性（p53的转录活性）或Western-blot、免疫共沉淀测定细胞内p53蛋白水平。

Method Transfection , reporter assay , western blot and autoradiography were performed to determine p53 transcriptional activity and its protein level .

瞬时转染SARS-CovM基因对大鼠肺成纤维细胞生长特性及生物学功能的影响

Influence on the growth and biological function of cultured rat lung fibroblasts transfected by SARS-CoV M gene

方法构建A类清道夫受体N端第1～27位氨基酸胞浆结构域缺失体的表达质粒，并将其经脂质体介导瞬时转染入CHO细胞中。

Methods A SR-A DNA mutant ( SR-A Δ _ 1-27 ) was constructed by truncating the 1 to 27 amino acid residues of the N-terminal cytoplasmic domain .

瞬时转染Arresten基因对血管内皮细胞迁移的影响

Inhibition of endothelial cell migration by transient transfection of Arresten gene

采用瞬时转染的方法，通过荧光素酶报告基因实验检测eNOS基因转录起始点上游长16kb启动子区域的活性。

Promoter activity of eNOS gene was determined by luciferase reporter gene assay .

通过瞬时转染PC12细胞实验研究不同截断突变体的促神经细胞分化功能。

The neuron differentiation function of different truncated mutants was observed by PC12 transient transfection assay .

瞬时转染293T细胞后收集含融合蛋白的细胞培养上清；

After transient expression , the cell supernatants of293T cells containing the aimed protein were obtained .

以其瞬时转染COS7细胞，应用RTPCR、细胞免疫荧光技术，及夹心ELISA检测融合蛋白的表达。

The recombination plasmid was transiently transfected into COS7 cells and the expression of the fusion protein was identified by RT PCR , immunofluorescent assay and sandwich ELISA .

目的：以CHO-K1细胞为宿主基因瞬时转染条件的优化。

Objective : To optimize the condition of gene transient transfection in CHO-K1 cell .

CHO-K1细胞中基因瞬时转染的条件优化

Optimization of Gene Transient Transfection in CHO-K1 Cell

以MDR1entryclone质粒瞬时转染MCF-7/ADM细胞株，MTT法测细胞对ADM的耐药性及流式细胞仪测试P-gp表达情况。

MCF-7 / ADM was transfected with entry clone plasmid , and MTT method was used for detecting cell drug resistance and flow cytometer was used to examine the expression of P-gp .

结果K562细胞瞬时转染MDM2siRNA48h后，MDM2蛋白质表达下降超过60%；

Results After 48 h of transient transfection with the MDM2 siRNA the results of Western blot showed that expression of MDM2 protein were knockdowned over 60 % in K562 cells .

结果：瞬时转染的神经胶质细胞中GLT-1基因的表达受到明显抑制，GLT-1蛋白含量明显下降。

Results : The distinct inhibition of the expression of GLT-1 in the transfected glial cells was observed .

流式细胞仪及WST-8法筛选荧光标记的PEI与ASODN在不同质量比时的瞬时转染效率及48h转染效率；

The instantaneous and 48 h transfection efficiencies of PEI were determined by flow cytometry and WST-8 test in various ratios of PEI / ASODN .

GFPeIF5A瞬时转染后，起初eIF5A为全细胞分布，以后逐渐转移到细胞质中。

The results of transient transfection showed that GFP-eIF-5A was distributed over whole cell at the beginning of its expression , but was gradually concentrated in cytoplasm .

瞬时转染CHO、Hela、UMR-106细胞，检测荧光素酶的活性，分析不同长度的启动子片段在各种细胞中的转录活性。

These report vectors were transiently transfected into CHO , Hela and UMR - 106 cells and luciferase assay was performed to analyse the transcription activation of these promoters .

获得的瞬时转染重组质粒的细胞系，经实时定量PCR鉴定1nicroRNA-21的表达水平与正常甲状腺乳头状癌细胞TPC-1相比明显升高（P0.01）。

The obtained recombinant plasmids were transiently transfected cell lines , compared by real-time quantitative PCR identification of microRNA-21 expression levels and normal thyroid papillary cancer cells TPC-1 was significantly increased ( P0.01 ) .

目的研究野生型PTEN体外瞬时转染对人乳腺癌MCF7细胞的生长抑制作用和对阿霉素敏感性的增效作用。

Objective To study whether transient overexpression of tumor suppressor gene PTEN could lead to growth suppression and up-regulate the sensitivity to doxorubicin of human breast cancer MCF-7 cells .

收集瞬时转染及筛选后的细胞培养基，用酶连结免疫吸附分析（ELISA）检测试剂盒检测人B型利钠肽（hBNP）的表达水平。

After transfected and during selected , the concentration of human B-type natriuretic peptide ( hBNP ) in cell culture medium was detected by enzyme-link immunosorbent assay ( ELISA ) .

结果瞬时转染70h细胞有目的蛋白表达。

Results Target protein was expressed in the CHO-dhfr ~ - cells 70 h after transient transfection .

脂质体lipofectamineTM2000介导瞬时转染，通过RT-PCR检测人胶质瘤细胞系BT-325细胞中ClC-2mRNA表达。

The plasmids were transfected transiently into human glioma cell line BT-325 cells by Lipofectamine ~ ( TM ) 2000.The mRNA expression of ClC-2 gene was detected by ( RT-PCR . )

目的：克隆并构建含人白介素12（hIL-12）基因p35、p40不同亚基的真核表达载体，瞬时转染真核细胞并诱导IL-12的表达。

AIM : To clone and construct the eukaryotic expression plasmids containing different subunits of human IL-12 and to investigate its expression following transient transfection into COS-7 cells .

K16-RGD介导TGF-β1基因瞬时转染效率可达（21.6±4.3）%。

The transient transfection efficiency of TGF - β 1 gene mediated by K16-RGD was about 18.6 % .

结果：EGFP瞬时转染效率为30%～40%，稳定转染效率约1104～5，且稳定转染的人胚胎干细胞均表达EGFP。

Results : Transient transfection efficiency was 30 % ~ 40 % , while stable transfection efficiency was about 1 / 10 4 ~ 5 . Furthermore , all stably transfected human embryonic stem cells expressed EGFP .

构建不同YY1结合位点突变的HPV16LCRCAT报道质粒，体外瞬时转染真核细胞，提取胞质蛋白，以建立的方法进行CAT表达检测。

Several CAT reporter plasmids containing different HPV 16 LCR sequences were generated and transfected transiently to monolayer cells in vitro . The cytoplasm proteins were extracted and the expressions of CAT were evaluated with the newly established ELISA assay .

结果：（1）脂质体法将FAM标记的阴性对照siRNA瞬时转染大鼠海马神经元，流式细胞仪检测转染效率为34.3%。

The localization of FAM and neurite outgrowth of hippocampal neurons were observed by fluorescence microscope . Results : ( 1 ) The negative control FAM-siRNA was transfected successfully with lipofection reagent and transfection efficiency tested by Flow cytometry came up to 34.3 % .

目的探讨造影剂声诺维（SonoVue）联合超声辐照人脐血管内皮细胞（HUVEC）时增强型绿色荧光蛋白报告基因质粒（pEGFP）的瞬时转染效率。

Objective To investigate the transfection efficiency of plasmid vector coding enhanced green fluorescence protein ( pEGFP ) in human umbilical vein endothelial cell ( HUVEC ) by using ultrasound exposure and ultrasound contrast agent ( SonoVue ) .