相差显微镜

方法：1.体外培养人视网膜色素上皮细胞（humanretinalpigmentepithelialcell，hRPEcell），并通过倒置相差显微镜观察其生长情况。

Observe the situation of human retinal pigment epithelial cell ( hRPE cell ) cultured in vitro thougth inverted phase contrast microscope . 2 .

本研究采用相差显微镜、透射电镜、扫描电镜等技术观察细胞形态结构，采用MTT法研究血清和血清替代品对ICE6细胞生长的影响。

In this study , phase contrast microscope , transmission and scan electron microscope were applied to observe the form and constructure ;

通过相差显微镜观察CAI处理后MCF-7细胞的形态学变化。

The morphological changes of MCF-7 cells were observed and photographed by a phase-contrast microscope .

利用扫描电子显微镜（SEM）、相差显微镜和动态热机械分析（DMA）表征CR/BR共混物的相态结构；

SEM , phase contrast optical microscopy , DMA were used to prove the phase structure of the blends .

在相差显微镜下动态观察培养AML细胞的形态和活力。

Morphologies and viability of cultured AML cells were examined every day under phase contrast microscopy .

方法：微孔滤膜过滤、乙酸乙酯福尔马林分离、Giemsa染色、油镜及相差显微镜观察。

Methods : Micromembrane filtration , ethyl acetate-formalin separation , giemsa stain .

以倒置相差显微镜观察PC12细胞神经突起的变化；

The morphology of PC12 cells was observed by phase-contrast microscopy .

以相差显微镜及成像分析技术测定纤毛运动频率（CBF）。

Ciliary beating frequency ( CBF ) was measured by phase contrast microscope and videotape analysis .

用倒置相差显微镜和荧光显微镜观察标记细胞的形态和GFP表达。

Morph and expression of GFP of labeled NSCs were observed with invert phase-contrast light microscope and fluorescence microscope respectively .

方法：1.采用胰酶消化法获得Wistar大鼠CFs。采用倒置相差显微镜和波形蛋白免疫荧光法鉴定细胞。

CFs were isolated by trypsin digestion method from Wistar rats and identified by invert microscope and vimentin immunofluorescence . 2 .

方法依据ISO国际标准[1]，采用体外细胞培养四甲基偶氮唑盐比色法和倒置相差显微镜形态观察法对新型粘接剂的生物相容性进行评价。

Methods According to ISO-7406 criterion , MTT-assay and Inverted Microscope for the culture cells in vitro were used to evaluate the compatibility of a new adhesive .

采用密炼机在线取样，相差显微镜拍照研究乙烯-1-辛烯共聚物（POE）在聚丙烯（PP）中的相分散行为。

Dispersing behavior of poly ( octene-1-ethylene )( POE ) in polypropylene ( PP ) matrix were investigated in mixer with on-line analysis by phase contrast microscope .

相差显微镜观察两者联合作用12h大量细胞固缩变圆，随时间延长胞体萎缩裂解。

Major cells shrunk in 12 h under phase-contrast microscopy , and cell body became atrophic and split with prolonged time .

相差显微镜观察凋亡细胞的形态学改变，流式细胞仪（FCM）检测细胞凋亡率及ΔΨm变化；

The cells were examined by phase contrast microscopy , flow cytometry ( FCM ) for evidence of apoptosis , and also by FCM for ΔΨ m.

取培养3、6d的细胞-支架复合物的标本，用倒置相差显微镜和扫描电镜观察细胞的形态及在支架上的粘附、生长情况。

The cells growth on the scaffolds was observed by scanning electron microscope ( SEM ) .

结果：经9d诱导培养，细胞数量增加，体积增大，相差显微镜、HE染色和扫描显微镜下均见细胞表面树突状突起，呈典型DC形态。

RESULTS : After 9-day culture , the size and quantity of the cells increased . Dendritic structure was observed under microscope and SEM .

第二部分1.将原代培养的SD大鼠心肌细胞或内皮细胞接种于petri皿中，倒置相差显微镜下观察细胞形态。

Primary myocardial cells and endothelial cells from the Sprague Dawley rats were cultured and seeded in the petri dishes . Cells morphology were observed by inverted phase contrast microscop . 2 .

方法用不同浓度的BOD处理体外培养的人肺癌A549细胞，应用倒置相差显微镜、MTT分析方法检测A549细胞形态学变化及细胞存活率的变化。

Methods After treating A549 cells with different concentrations of BOD , light microscopy and MTT assay were used to determine morphological changes and cell viability respectively .

方法选取肾病内科住院病人血尿患者102人，分别用UF-100尿沉渣分析仪与相差显微镜检查分析尿液中红细胞的相关参数。

Methods Urinary samples from 102 inpatients with kidney disease were examined by UF-100 and phase-contrast microscopy respectively .

用相差显微镜观察形态学，DNA琼脂糖凝胶电泳和Hoechst33258核染色分析神经元凋亡，二乙酸荧光素（FDA）染色法检测细胞的存活率。

Morphology of neurons was observed by phase-contrast microscopy and Hoechst 33258 nucleus staining . The neuronal viability was measured by fluorescein diacetate ( FDA ) staining . DNA fragmentation was analyzed by agarose gel electrophoresis .

方法：运用电镜和相差显微镜观察UU人工感染正常精子和未感染精子24h后的形态学改变。

Methods : Using electron microscope and phase contrast microscope , we observed morphological changes of sperm infected for 24 hour by UU and no infected by UU .

通过溶解试验、电子显微镜、相差显微镜、元素分析、IR和NMR分析了共聚物的结构，证明该共聚物主要为嵌段体。

Its structure was identified by means of solubility test , IR , NMR , element analysis , electron microscope and phase contrast microscope , and the copolymer was proved to be mainly a block one .

结果对于血尿来源，UF-100尿沉渣分析仪检查的敏感性（93.1%）高于相差显微镜检查的敏感性（83.3%），P0.05；

Results The sensitivity of identification of origin UF-100 was 93.1 % , which was significantly higher than that 83.3 % of the phase-contrast microscopy ( P 0.05 );

倒置相差显微镜及透射电镜观察SGC7901细胞形态学变化。

Invert phase-contrast and transmission electron microscopy were used to observe the morphological variations of apoptotic cells . 3 .

倒置相差显微镜对滑膜细胞进行形态学观察，免疫细胞化学（SABC法）染色对滑膜成纤维细胞进行鉴定。

Inverted microscope was employed to observe the synovial cells and immunocytochemistry ( SABC method ) staining was used to identified synovial fibroblasts .

方法将100μg/ml大蒜素直接加入肿瘤细胞培养6h，用相差显微镜观察肿瘤细胞形态学变化。

Methods 100 μ g / ml allicin was added to the tumor cell culture for 6h , the morphology of tumor cells was observed by discrepancy microscope .

观察加药前后HEC-1B细胞的形态学变化。使用倒置相差显微镜。

Observe the morphology changes of HEC-1-B cells . Using inverted phase contrast microscope . 4 .

结论UF-100与相差显微镜结合使用是血尿来源鉴别的最好策略。

Conclusion The best strategy is to combine UF-100 and phase contrast microscopy for the localization of the sites of hematuria .

方法利用GFP-腺病毒空载体感染NSCs（NSCs-GFP），经G418筛选并连续传代，在相差显微镜下观察未感染NSCs和感染NSCs的形态以及在荧光显微镜下观察感染NSCs及其诱导分化后的GFP表达情况；

After the infected cells were selected by G418 , GFP expression of NSCs-GFP and differentiated NSCs-GFP were detected under phase contrast microscope and fluorescent microscope .

方法选取肾内科门诊病例95例和住院病例86例，分别用UF-100与相差显微镜检查分析尿液中红细胞的信息。

Methods Urine samples from 95 out patients and 86 in patients with kidney disease were measured by UF-100 and phase-contrast microscopy .