皇家嘎拉

以皇家嘎拉苹果的叶片为转化受体，通过根癌农杆菌介导将铁结合蛋白（Ferritin）基因导入受体中，诱导获得了抗性芽。

The bean iron-binding gene ( Ferritin ) was introduced into the leaves of Royal Gala apple cultivar by Agrobacterium-mediated genetic transformation , and some Kanamycin-resistant shoots were obtained .

同源四倍体皇家嘎拉苹果的生长结果特性

Growth and fruit characteristics of autotetraploid plants of Royal Gala apple

本试验首先以皇家嘎拉苹果叶片为试材，采用三种方法提取了总RNA并加以比较。

At first , the three methods were adopted to extract RNA from apple leaves .

比较了三种方法提取皇家嘎拉苹果叶片总RNA的效果，发现改进的十二烷基硫酸钠-热酚法可提出高质量的RNA，且成本低廉，最终选定此法用于实验。

Three methods were adopted to extract RNA from apple leaves , the results showed that SDS-Hot Hydroxybenzene method was better than others .

茎段外植体白化处理促进皇家嘎拉苹果不定芽再生

Etiolation of stem explants benefits adventitious bud regeneration of ' Royal Gala ' apple

秋水仙素处理离体叶片获得皇家嘎拉苹果四倍体植株

Regeneration of Tetraploid Plants of Royal Gala Apple Variety From in vitro Leave Treated with Colchicine

同时，本研究建立了生产示范基地，为‘太平洋嘎拉’进一步的开发工作提供了有利的技术支撑。主要研究结果如下：1.‘太平洋嘎拉’来源于‘皇家嘎拉’的一个优良的早熟芽变。

At the same time , this research established the production bases , which offer the technical support for a further development of ' Pacific Gala ' . The main findings as following : 1 .

为了解皇家嘎拉苹果四倍体与二倍体对照植株在生长结果特性方面的差异，取2种果树的接穗高接到10年生大树上，在植株结果后进行了调查研究。

In order to understand their difference in growth and fruit characteristics , the scions from the two kinds of plant were grafted onto the 10-year-old apple trees , investigation were carried out after the trees began to bear fruit .