溴化乙锭
- 名ethidium bromide
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溴化乙锭在毛细管DNA传感器中的应用
Application of ethidium bromide in capillary DNA biosensor
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样本加样RNA量用溴化乙锭染色法评估。
The amount of transferred RNA was estimated using the ethidium bromide staining method .
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通过用溴化乙锭荧光法分析了这类配合物与DNA的相互作用,初步筛选了它们的抗癌活性。
Their antitumor activity was studied by ethidium fluorescence spectroscopy .
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以溴化乙锭作为与DNA结合的荧光探针,考察PB对其结合体系的影响。
Using ethidium bromide ( EB ) as a fluorescent probe , the binding of PB with DNA was studied .
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目的建立比溴化乙锭(EB)更为安全的核酸染色方法。
OBJECTIVE To set-up safe alternatives to ethidium bromide ( EB ) .
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用溴化乙锭作为荧光探针,还研究了La(Ⅲ)-L配合物与小牛胸腺DNA的相互作用的过程。
Using ethidium bromide as a fluorescence probe , the binding mode of the lanthanum (ⅲ) complex with calf-thymus DNA was studied spectroscopically .
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此外以溴化乙锭(EB)为荧光探针,对配合物与DNA的结合机理进行了探讨。
Moreover the mechanism of the interaction between the complex and DNA was also investigated using EB as a fluorescence probe .
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以溴化乙锭为荧光探针,利用荧光光谱法研究了枸橼酸氯米芬与脱氧核糖核酸(DNA)之间的相互作用。
The interaction between clomifene citrate ( CC ) and deoxyribonucleic acid ( DNA ) was studied by fluorescence spectroscopy using ethidium bromide ( EB ) as a fluorescence probe .
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吖啶橙/溴化乙锭(AO/EB)双荧光染色法及电镜(TEM)下观察细胞凋亡的形态学变化;
Additionally , HEC-B cell morphology changes of apoptosis were observed by AO / EB double fluorescent dye staining and by TEM .
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以溴化乙锭(EB)为荧光探针,研究了7-羟基黄酮及磷酰化7-羟基黄酮与DNA的弱相互作用。
The non-covalent interaction of 7-hydroxy flavone and its phosphate with DNA was studied using ethidium bromide ( EB ) as a probe .
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由溴化乙锭从小沟方向插入不同碱基对的结合能考察发现,溴化乙锭对DNA碱基对有特异性识别,并且与CA碱基对结合能最强。
Comparing the binding energy of EB with different base pairs , it was found that EB had special recognition among base pairs with the maximum binding energy with CA.
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方法应用溴化乙锭荧光法测定加或不加S9条件下AN对牛胸腺DNA交联作用。
Method DNA cross-link effect of AN in calf thymus with or without S9 were measured by EB fluorescence assay .
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结果表明,NC中溴化乙锭插入DNA的成分比TC增加了486%;
The results show that the component with ethidium bromide inserting DNA in tumoral chromatin ( TC ) increases by 4.86 % than that in normal chromatin ( NC ) .
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结果对ANC强抗性突变株DM104对化学特性不相关的化合物如溴化乙锭和诺丹明产生交叉抗性。
Results These mutants were cross resistant towards chemically unrelated compounds such as ethidium bromide and rhodamine .
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丫啶橙(AO)/溴化乙锭(EB)染色荧光显微镜观察thapsigargin处理后K562/A02细胞形态改变;
Morphological change of K562 / A02 cells treated with thapsigargin was examined with AO / EB fluorescent staining under fluorescent microscopy .
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方法:保护薇乔缝线反复浸于反义核酸溶液中72h,溴化乙锭(EB)标记,紫外灯下观察其吸附反义基因的情况。
Methods : The Coated Vicryl was soaked in antisense oligonucleotide solution repeatedly for 72 h then marked with EB solution and observed under ultraviolet lamp .
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综合两组脉冲电泳的电泳条件、溴化乙锭染色以及DNA杂交分析结果,草菇染色体DNA至少可分为9个谱带。
Based on the conditions of 2 sets of pulsed field gel electrophoresis , the results of ethidium bromide staining and DNA hybridization analysis with two moderately repetitive probes , it was concluded that at least 9 chromosomal DNAs were found in V. volvacea .
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溴化乙锭(EB)(0.6μg/ml)在24h内能强烈地诱导S1回复发光,引起S1的回复发光突变,显著提高了S1的回复发光突变率。
Ethidium bromide ( EB ) ( 0 . 6 ug / ml ) can strongly induce dark variant ( S1 ) reversion luminescence , cause reversion mutation and significantly increase reversion rate .
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方法将Feulgen法、甲基绿染色法、全染料(stainsall)法和银染法等几种非荧光DNA特异染色方法试用于彗星试验,并与溴化乙锭(EB)染色进行了比较。
Methods Some special un_fluorescent DNA staining methods , Feulgen , Methyl Green , Stains_all and Silver_Stain were tested in Comet Assay and the comparison studies with EB stain were carried out .
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溴化乙锭(EB)常用于指示小分子与DNA作用方式研究,与其相比,Ru(bipy)2dppx2+具有灵敏度高、毒性低、稳定性好、选择性好、使用方便等优点。
Compared with normal DNA interacting mode spectroscopic probe EB , Ru ( bipy ) 2dppx2 + has the advantages of higher sensitivity , lower toxicity , higher stability and simplicity .
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利用荧光光谱、紫外-可见吸收光谱、圆二色谱、琼脂糖凝胶电泳方法研究了小檗碱(berberine)和溴化乙锭(EB)与ct-DNA的光谱学作用。
The spectroscopy interactions between berberine and DNA , ethidium bromide and DNA are studied by UV-absorption , fluorescence , circular dichroism spectroscopy and agarose gel electrophoresis methods .
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溴化乙锭及放线菌素D对高分辨染色体的作用
Effect of ethidium bromide and actinomycin D on high resolution chromosome G-banding
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丫啶橙/溴化乙锭(AO/EB)染色法检测细胞凋亡;
Spontaneous apoptosis of cells was detected by AO / EB dyeing .
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溴化乙锭探针研究劳氏青莲与脱氧核糖核酸的相互作用
Studies on the Interaction between Thionine and Deoxyribo-Nucleic Acid by Ethidium Bromide Probe
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溴化乙锭诱导发光细菌暗变株回复突变的研究
Study on Genie Mutation Induced by Ethidium Bromide in Dark Variant of Luminous Bacteria
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大豆核染色体、叶绿体DNA-溴化乙锭复合体某些萤光特性的比较研究
A comparative study of some fluorescence properties of soybean nucleus and chloroplast DNA-ETHIDIUM bromide complexes
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扩增产物在1-8%的琼脂糖凝胶上进行电泳分离,溴化乙锭染色。
Amplified IPCR products were evaluated by1.8 % agarose gel electrophoresis and ethidium bromide staining .
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溴化乙锭的新应用:均相检测羟自由基的新型荧光探针
New Application of Ethidium Bromide : A Novel Fluorescence Probe for Homogeneous Detection of Hydroxy Radical
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经12%非变性聚丙烯酰胺凝胶电泳,溴化乙锭染色,紫外透射分析仪下观察并拍照。
After 12 % native polyacrylamide gel electrophoresis , ethidium bromide staining , observed and took photographs with ultra-violet transmission analysator .
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扩增产物以1.5%琼脂糖凝胶(含0.5μg/ml溴化乙锭)电泳分离。
Amplification products were separated by 1.5 % agarose gel ( contain 0.5 μ g / ml ethidium bromide ) electrophoresis .