氯霉素乙酰转移酶

通过设计4个引物进行重叠（overlapping）PCR，由此克隆了人肿瘤坏死因子受体I死亡域（Deathdomain）与氯霉素乙酰转移酶（CAT）的融合蛋白基因（DdLcat）。

By designing four primers for an overlapping PCR , we created a fusion gene Ddl cat encoding human TNF receptor I death domain and chloramphenicol acetyltransferase ( CAT ) .

BGC-Ha-ras癌基因上游区片段对细菌氯霉素乙酰转移酶报告基因表达的促进作用

The Enhancer-like Effect of the Upstream Fragment of the BGC-Ha-ras Oncogene to the Expression of Bacterial Chloramphenicol Acetyltransferase Gene

氯霉素乙酰转移酶检测（CATassay）

Chloramphenicol Acetyl Transferase Assay ( CAT Assay )

不同种类的抗性细菌可以产生不同种类的氯霉素乙酰转移酶，其中以CATⅠ，Ⅱ，Ⅲ研究的最多，这三者中又以CATⅠ对底物的亲合性为最高。

Variants of chloramphenicol acetyltransferase come from a variety of bacterial species . The more attention is paid on CAT I , IK III , among which CAT I has the highest substrate affinity .

氯霉素乙酰转移酶突变体检测氯霉素

A New Method for Detecting the Chloramphenicol with the Mutanted Chloramphenicol Acetyltransferase

氯霉素乙酰基转移酶双抗体夹心ELISA检测方法的建立

Establishment of a sandwich ELISA method for detection of reporter chloramphenicol acetyltransferase gene

目的对氯霉素乙酰基转移酶（Chloramphenicolacetyltransferase，CAT）基因进行表达和纯化。

Objective To express and purify chloramphenicol acetyltransferase ( CAT ) gene .

方法：构建c-fos启动区氯霉素乙酰化转移酶（chloramphenicolacetyl-transferase，CAT），然后转染HeLa癌细胞株，用不同浓度SeO2处理后观察细胞中CAT的活性。

METHODS : HeLa cells were transfected with plasmids containing upstream regulating regions of c fos chloramphenicol acetyl transferase ( CAT ) .

方法构建针对氯霉素（Cm）乙酰转移酶（cat）并含卡那霉素（Km）抗性基因筛选指标的EGS重组质粒以及只含Km抗性基因但不含EGS的对照质粒。

Methods Recombinant EGS plasmids directed against Cm acetyl transferase ( cat ) and containing kanamycin ( Km ) drug-resistance gene and control plasmids only containing kanamycin-resistance gene without EGS were constructed .

方法32P随机引物标记氯霉素耐药基因为探针，以SlotBlot、Southernblot法检测，同时以氯霉素乙酰转移酶活性（CAT）和药敏试验（MIC）作为耐药标志。

Methods Detection was carried out by slot blot and southern blot , using Cm resistance gene labeled with ~ ( 32 ) P by random primed method as the probe . CAT and MIC were exploited as the marker of drug resistance .