核苷酸转移酶

原位末端脱氧核糖核苷酸转移酶介导dUTP标记技术观察凋亡细胞。

The cell apoptosis was observed via in situ terminal deoxyribonucleotide transferase-medicated dUTP labeling technique .

方法：采用免疫组化和末端脱氧核糖核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）法检测Bax和Bcl-2基因蛋白及凋亡细胞。

Methods : Bax and Bcl-2 genes protein expression and cardiomyocyte apoptosis were tested by immunohistochemical method and in situ TdT-mediated dUTP nick end labeling ( TUNEL ) .

照射后12h，用末端脱氧核苷酸转移酶介导的切口末端标记法检测海马齿状回凋亡细胞；

After radiation for 12 hours , the apoptosis in hippocampal dentate gyrus was detected with the cutting end labeling method mediated by terminal deoxynucleotidyl transferase .

终未脱氧核糖核苷酸转移酶介导的原位缺口末端标记（TUNEL）法结合图像分析技术检测左室心肌细胞凋亡；

Combined with automatic imaging analysis technique , cardiac myocytes ' apoptosis in left ventricle was detected by using in situ terminal deoxynucleotidyl transferase mediated dUTP nick end labeling ( TUNEL ) staining .

方法：采用瑞－姬染色和末端脱氧核苷酸转移酶（TdT）介导的脱氧核苷酸原位末端标记法（TUNEL）检测凋亡生精细胞。

Methods : Apoptotic spermatogenic cells were examined by Wright Giemsa staining and terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate ( dUTP ) biotin nick end labeling ( TUNEL ) technique .

方法：对51例CA和18例正常上皮分别采用免疫组化ABC法检测Fas抗原、末端脱氧核苷酸转移酶介导的dUTP缺口末端标记技术检测细胞凋亡。

Methods : The expression of keratinocyte Fas antigen and apoptosis in 51 cases of CA and 18 normal controls was detected respectively with immunohistochemical staining ( ABC method ) and terminal deoxynucleotidyl transferase-mediated dUTP nick endlabeling .

采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法（TUNEL）检测肺泡上皮细胞凋亡情况；用重力法测定血管外肺水（EVLW）含量；

Extravascular lung water ( EVLW ) was measured by gravimetric method . Alveolar epithelium apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end labeling ( TUNEL ) .

以透射电子显微镜、末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）法和流式细胞术分别检测调亡细胞的形态学变化、DNA断裂情况和不同细胞周期的DNA含量变化。

The morphological change , DNA breakage and the change of DNA content of the apoptotic cells in different cell cycles were detected by Transmission electronic microscope , Terminal deoxynucleotidyl transferase mediated dUTP nick end labeling ( TUNEL ) assay and flow cytometry .

采用原位PANT（DNA聚合酶I介导的生物素标记的dATP缺口平移）及原位TUNEL（末端脱氧核苷酸转移酶介导的dUTP末端标记），分别检测DNA损伤的单链断裂及双链断裂。

DNA polymerase I mediated biotin dATP nick translation ( PANT ) and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling ( TUNEL ) were adopted for in situ identification of single and double strand DNA fragmentation , respectively .

方法用原位末端脱氧核糖核苷酸转移酶介导的dUTP缺口末端标记（TUNEL），DNA梯子和体外培养等方法对38例MDS患者骨髓单个核细胞（BMMC）凋亡进行研究。

Methods Apoptosis of bone marrow mononuclear cells ( BMMC ) from 38 MDS patients was studied by TdT mediated dUTP nick end labeling ( TUNEL ) in situ , DNA ladder and shortterm BMMC culture in vitro .

方法分别采用端粒酶活性试剂盒和末端脱氧核苷酸转移酶介导缺口末端标记（TUNEL）法对56例膀胱癌标本进行端粒酶活性和细胞凋亡的检测，并结合临床资料进行分析。

[ WT5HZ ] Methods [ WT5BZ ] The intensity of telomerase activity and cellular apoptosis of bladder cancer were detected by telomeric repeat amplification protocol ( TRAP ) and terminal deoxynucleotidyl transferase mediated dUTP biotin nick end labeling ( TUNEL ) assay respectively .

本文采用Okamura氏的寡聚脱氧胸苷酸（oligo-dT）-纤维素柱层析法分离纯化小牛胸腺末端脱氧核苷酸转移酶（TdT）。

Present paper used Okamura 's method of oligo dT-cellulose affinity chromato - . graphy to isolate and purify terminal deoxynucleotidyltransferase ( TdT ) from calf thymus . The procedure consists of : extraction of crude enzyme ;

胸腺激素对末端脱氧核苷酸转移酶的影响

The effect of thymic hormone on terminal deoxynucleotidyl transferase

末端脱氧核苷酸转移酶输卵管内核前阶段移植

Pronuclear stage tubal transfer

应用DNA3末端转移推测死亡时间胸腺淋巴细胞末端脱氧核苷酸转移酶和脱氧核糖核酸α-聚合酶活性的部分抑制

Estimation of Postmortem Intervals by Using Terminal Deoxynucleotidyl Transferase ; Partial Inhition of DNA Polymerase and Terminal Deoxyribonucleotide Transferase Activities from Bovine Thymus Lymphocytes

各组分别于相应时间点取脑组织，然后应用末端脱氧核苷酸转移酶介导的脱氧三磷酸尿苷缺口末端标记法测定大鼠大脑皮层、海马神经细胞凋亡数目。

Brain tissues were obtained at corresponding time points in each group , respectively , and then the cerebral cortex and apoptotic number of nerve cells in hippocampus were measured with terminal deoxynucleotidyl transferase ( TdT ) mediated Dutp nick end labeling ( TUNEL ) .

DNA聚合酶mu（polμ）基因仅存在于哺乳动物基因组中，是脱氧核苷酸末端转移酶（TdT）的重复基因；

Evidence also shows that DNA polymerase mu ( pol μ) is only present in mammals , and is a duplicated gene of terminal deoxynucleotidyl transferase ( TdT ) .

应用脱氧核糖核苷酸末端转移酶介导的dUTP缺口末端标记技术（TUNEL）对TGF-β1诱导的肝癌细胞的凋亡进行定量检测；

TGF - β 1-induced apoptosis in hepatic carcinoma cell lines was measured by the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling ( TUNEL ) assay .

应用光镜、核苷酸末端转移酶介导的dUTP缺口标记法观察各组小鼠视网膜组织结构的改变；

Morphologic changes of photoreceptor cells in different group were examined by light microscope and the apoptosis was detected by TdT-mediated dUTP nick end labeling .

分别按期摘取眼球，制备视网膜消化铺片微血管标本，PAS染色及核苷酸末端转移酶介导dUTP缺口翻译法（TUNEL法）特异性标记凋亡细胞，光镜下观察。

Eyeballs were selected separately , retinals digesting stretched preparation capillary samples were prepared , and apoptosis cells were specificity marked by PAS dyeing and ribonucleotide terminal transferase mediating dUTP nick-translation ( TUNEL method ), to observe below light microscope .

本研究选用了三种含有不同p53基因状态的人肝癌细胞系，应用脱氧核糖核苷酸末端转移酶介导的dUTP缺口末端标记技术（TUNEL）对TGF-β1诱导的肝癌细胞的凋亡进行了定量检测。

Three human hepatic carcinoma cell lines , involving different status of the p53 gene respectively , was used in this study . TGF - β 1-induced apoptosis in hepatic carcinoma cell lines was quantitated using the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling ( TUNEL ) assay .