柠檬酸合成酶

人柠檬酸合成酶cDNA的克隆及其表达谱分析

Cloning and tissue expression pattern analysis of the human citrate synthase cDNA

有人已经证实哺乳动物组织的柠檬酸合成酶受ATP的抑制。

The citrate synthase of mammalian tissues have been shown to be inhibited by ATP .

根据烟草中柠檬酸合成酶（citratesynthase，cs）基因的序列设计两个上下游引物，并在上下游引物中分别加入NcoⅠ和XbaⅠ酶切位点。

Primers were designed according to the nucleotide sequence of citrate synthase ( cs ) gene .

结果表明：①从6～8月柠檬酸合成酶（CS）活性迅速增加，8月中旬达到高峰，随后迅速下降，在10月中旬其活性出现回升后又再次下降；

The results showed : ① From June to August , the activity of citrate synthetase ( CS ) increased rapidly and reached the maximum in middle of August , then decreased rapidly . In middle of October , CS activity returned to rise and decreased again .

方法：测定通过口服途径亚慢性暴露于百草枯、MPTP或等量生理盐水的小鼠脑内线粒体呼吸链中复合酶Ⅰ、Ⅱ、Ⅳ及柠檬酸合成酶的活性。

Methods : Activities of complex ⅰ, complex ⅱ, complex ⅳ and citrate synthase were measured after mice exposed to paraquat or MPTP or the same amount normal saline administered orally every day for 2 months .

结果表明：HC-3强烈地抑制柠檬酸合成酶（CS）活性，使果实中有机酸含量极显著降低，同时增加了果实中的总糖含量，因此在生产中具有推广应用价值。

The results show that HC-3 inhibited CS activity of treated fruit , the organic acid content of fruit in treatment is lower than that in control . Besides , the sucrose content in fruit increases by HC-3 spray . So HC-3 is to be practically valuable .

烟草柠檬酸合成酶基因的克隆及其光诱导型植物表达载体的构建

Cloning of tobacco citrate synthase cDNA and construction of its light inducible plant expression vector

其中，酶学水平上，比较了两菌株在初级代谢过程中的四种关键酶酶活，分别是葡萄糖-6-磷酸脱氢酶、柠檬酸合成酶、异柠檬酸裂解酶和苹果酸合成酶。

Glucose-6-phosphate dehydrogenase , citrate synthase , isocitrate lyase and malate synthetase are the four key enzymes involved in the primary metabolism .