杆状病毒表达系统

目的利用Bac-to-Bac杆状病毒表达系统，将人碱性成纤维细胞生长因子（humanbasicfibroblastgrowthfactor，hbFGF）基因进行表达，并检测重组蛋白的特异性及生物学活性。

Objective Bac-to-Bac baculovirus expression system was used to obtain biologically active recombinant human basic fibroblast growth factor ( hbFGF ) in insect cells .

结论：重组的BacTRⅡ杆状病毒表达系统可以用于正确表达TGFβRⅡ/Fc融合蛋白。

CONCLUSION : The recombinant Bac TR ⅱ baculovirus expression system can be correctly used to express chimeric protein TGF β R ⅱ / Fc .

棉铃虫组织蛋白酶B在杆状病毒表达系统中的表达及鉴定

Expression and identification of Helicoverpa armigera cathepsin B in baculovirus system

RNA干扰技术和家蚕杆状病毒表达系统在纤维素酶研究中的应用前景

Application Prospects of RNA Interference and Silkworm Baculovirus Expression System in Cellulase Research

呼吸道合胞病毒融合蛋白F在重组杆状病毒表达系统中的表达及抗原性研究

Expressions of the fusion protein F of respiratory syncytial virus in recombinant baculovirus and its antigenicity analysis

采用昆虫细胞杆状病毒表达系统，构建了中国HIV1gag基因的杆状病毒表达质粒，得到了表达HIV1E和B亚型gag的重组杆状病毒。

Using the Bac to Bac system , we obtained the recombinant baculoviruses containing gag genes .

低分子量尿激酶与AnnexinV的融合基因在家蚕杆状病毒表达系统中的表达

Expression of Prourokinase ( 144-411 ) - Annexin V Chimeras in Bombyx mori Baculovirus Expression Vector System

实验共分两部分：实验一：带有His标签的禽流感病毒HA基因在Bac-N-Blue杆状病毒表达系统中的表达。

Experment ⅰ: Study on expression of HA gene of avian influenza virus with 6 × His tag in Bac – N-Blue expression system of baculovirus .

论文的主要内容包括：（1）利用杆状病毒表达系统表达全长核受体PPARγ。

The principal contents of this thesis including : ( 1 ) expression of the full-length PPAR γ by BEVS .

[2]构建了能够表达多角体的重组BmNPV杆状病毒表达系统。

[ 2 ] A novel BmNPV expression system which can produce the polyhedrin was constructed .

应用BactoBac杆状病毒表达系统成功地表达了VP3基因并获得了具有良好免疫活性的重组蛋白。

Using Bac-to-Bac expression system , the recombinant protein has been achieved and proven to exhibit high immune activity .

神经营养素-4（NT-4）在昆虫杆状病毒表达系统中的克隆与表达

Expression of Neurotrophin-4 ( NT-4 ) Gene in Baculovirus Expression Vector System

目的建立昆虫杆状病毒表达系统表达人II型CD74胞外端片段的技术平台。

Objective To establish an expression system of human II CD74 extracellular fragment by insect baculovirus vector expression system in insect cells .

结果：1.用杆状病毒表达系统在昆虫细胞中成功表达了汉滩病毒G1和G2蛋白。表达的重组蛋白与病人血清和用灭活病毒免疫的兔血清均发生特异性反应。

The recombinant Gl and G2 proteins were expressed successfully in insect cells and the proteins reacted specifically with the HFRS patient sera and positive rabbit serum . 2 .

不同区段HIV-1env基因在Bac-to-Bac昆虫细胞杆状病毒表达系统中的表达及检测

Expression and Detection of Different Fragments of HIV-1 Envelope Genes in a Bac-to-Bac Baculovirus Insect cell Expression System

Bac-to-Bac策略是目前昆虫杆状病毒表达系统获取重组杆状病毒最为方便、快捷的途径之一。

The strategy of Bac-to-Bac is one of the most convenient and rapid ways to generate recombinant baculovirus .

方法：通过RT-PCR法扩增汉族人GDNF前体cDNA，利用昆虫杆状病毒表达系统（BES）表达此前体cDNA，原代培养中脑多巴胺能神经元对表达产物进行活性测定。

Methods : The Han nationality GDNF precursor cDNA was amplified by RT PCR and expressed by baculovirus expression system . The biological activity of the expressed product was measured by the primary culture of midbrain dopaminergic neurons .

以上结果显示：ChiA、CP两基因为病毒复制非必需基因，且通过ChiA、CP两基因的失活，使家蚕杆状病毒表达系统表达外源蛋白的水平有了一定的提高；

The above result showed : Chia and CP gene were not essential for virus replication , and by inactivating ChiA and CP gene , the expressing level of foreign protease of BEVS was improved .

本研究利用Bac-to-Bac重组杆状病毒表达系统在昆虫细胞系Sf21细胞中得到了HCV结构蛋白的成功表达，并在人宫颈癌细胞系HeLa细胞中检测到了E1区基因的转录。

In this work , we successfully expressed HCV structural proteins in insect cells by using recombinant baculovirus expression system and detected the transcription of E1 coding sequence in HeLa cells transduced by recombinant baculoviruses .

实验中选用了Bac-to-Bac杆状病毒表达系统，这是一种通过位点特异的转座作用产生用于表达的重组杆状病毒的系统。

The Bac-to-Bac ? Baculovirus expression system has been chosen to express these proteins . This is an efficient site-specific transposition system to generate Baculovirus for high-level expression of recombinant proteins .

证明了杆状病毒表达系统可以用来生产抗CSFV病毒的亚基疫苗或抗原，这将有利于对猪瘟病毒的发病机理和治疗方法的研究。

Our results provide evidence that bacmid-based baculovirus expression system could be used as an alternative strategy to develop the subunit vaccine or antigen against CSFV of swine , which will be beneficial to the mechanistic and therapeutic studies against swine virus .

利用杆状病毒表达系统表达金鱼生长激素I基因

Expression of Goldfish Growth Hormone I Gene Using Baculovirus Expression System

昆虫杆状病毒表达系统研究进展及其应用展望

Research Progress and Application Prospect of Insect Baculovirus Expression System

昆虫杆状病毒表达系统表达人载脂蛋白A-Ⅰ

Expression of Human Apolipoprotein A - ⅰ in Baculovirus - insect Cell System

半胱氨酸蛋白酶基因在家蚕杆状病毒表达系统中的表达

Expression of Cysteine Protease Gene in Bm-BmNPV Expression System

介绍了杆状病毒表达系统及其优点。

Baculovirus expressed system and its advantages were introduced .

杆状病毒表达系统的发展

Brief description of baculovirus expression system development

昆虫杆状病毒表达系统的研究及其新进展

Advances in Insect Baculovirus Expression System

杆状病毒表达系统是一个裂解性的系统，杆状病毒本身具有多个蛋白酶基因，在表达目的基因产物的同时，这些蛋白酶也得到了表达。

BEVS is a lytic expression system . There are several proteinase coded by the BmNPV itself .

利用昆虫杆状病毒表达系统方法来表达其主要的结构蛋白是获得有天然活性抗原的最有前途的方法。

The most promising method is using a baculovirus expression system , expressing major structural activity protein .