插入序列

插入序列IS2的研究&Ⅱ.突变体的极性效应和DNA序列分析

The Study of Insertion Sequence IS 2 ⅱ . Polarity Effect of the Mutant and DNA Sequence Analysis

幽门螺杆菌中国菌株插入序列IS607的分布

Distribution of Insertion Sequence 607 of Helicobacter Pylori for China Strains

我国税收基本法研究basic载体中，经测序鉴定插入序列的正确性。

Study on Basic Tax Law of China basic vector .

应用插入序列PCR进行大肠杆菌O（157）：H7基因分型的研究

Study on the application of insert sequence PCR in genotyping of E.coli O_ ( 157 ): H_7

起始单链DNA随机库为96个碱基短链核苷酸的混合物，其中含有60个随机插入序列。

The starting DNA pool was a mixture of 96-mer single-stranded DNA ( ssDNA ) molecules that had randomized 60-nucleotide inserts .

结果AD与LRP基因上游区域5′端插入序列多态性无关联；

Results There was no association between AD and the tetranucleotide polymorphism ;

经GeneBankBlast分析证实DNA插入序列与目的片段DNA序列完全完全一致。

The GeneBank Blast analysis confirmed that fragment of DNA insertion sequences and completely identical DNA sequences .

插入序列采用PstI和测序的方法来证实。

The inserted sequences were confirmed by Pst I digestion and sequencing .

注意，可以将零插入序列值，这通常适用于Informix串行类型。

Notice that zero is inserted into the serial value , as is usual for the Informix serial type .

结论首次发现大肠杆菌K99基因中含有插入序列IS1。

Conclusion Inserted sequence IS1 was observed in E.coli K99 gene for the first time .

该插入序列造成GRA1基因移码突变。

The frameshift mutation for GRA1 gene resulted from the insertion of exogenous sequence .

引物来自结核分支杆菌特异重复插入序列IS6110。

IS6110 , a specific insert sequence of M.tuberculosis , was used as primer .

插入序列在细菌产生新的O-抗原形式中也起重要作用，大肠杆菌O3，O15和O57的O-抗原基因簇都是在插入序列的作用下形成的。

It is shown that the O-antigen gene clusters of E.coli O3 , O15 and O57 are formed by the insertion sequences .

coli中，Sec的掺入需要在一个顺式作用元件&Sec插入序列（SECIS）和四种蛋白质（SelA，SelB，SelC，SelD）的共同参与下才能完成。

It was found that the incorporation of selenocysteine needs a cis-acting element ( SECIS ) and four proteins ( SelA , SelB , SelC , SelD ) in E. coli .

方法构建纳米颗粒信号放大载体，建立杂交信号放大方法，检测标本中结核分枝杆菌特异性的插入序列IS6110。

Methods Nanoparticle signal amplification carrier was prepared , based on which a signal amplification method was developed to detect the specific inserted sequence IS6110 in M.tuberculosis .

通过构建三个双元载体的质粒pDsE、pDsG和pAc和农杆菌介导的T-DNA转基因，发展了一个带有基因捕获器和增强子捕获器的Ac/Ds转座子系统作为插入序列标签来研究水稻基因组。

By constructing three binary vectors ( pDsE , pDsG and pAc ) and T-DNA transformation mediated by Agrobacterium , we developed an Ac / Ds system harboring gene trap and enhancer trap for gene tagging in rice .

该基因在3′-端非翻译区（3′-UTR）具有硒代半胱氨酸插入序列（SECIS）结构，其大小为102bp。

The gene contained a selenocysteine insertion sequences ( SECIS ) element in its 3 ′ - untranslated region ( 3 ' - UTR ) at the size of 102 bp .

携带耐药基因的基因转移元件有：质粒、转座子、整合型噬菌体和整合子，以及最近几年才发现的插入序列共同区（ISCR）。

There are plasmid , transposon , temperate phage , integron and insertion sequences common regions ( ISCR ), which can get and transfer the resistance genes .

对于基因分布和结构的分析显示这些基因变种是猪链球菌整合外源基因的结果。我们同样发现了插入序列和polyG等与猪链球菌基因交换相关的遗迹。

Our results suggested these variations resulted from integration of foreign genes and vestiges of genetic exchange such as insertion sequences and poly G were also found in some sequences .

结论布鲁杆菌插入序列IS711在基因分布上存在多态性。

Conclusions There was high genetic polymorphism with insert sequence 711 of Brucella .

目的：研究鼠疫耶尔森氏菌的插入序列IS1541的插入位点的特性。

Objective : Studying on the character of IS 1541 of Yersinia pestis .

结核分枝杆菌中插入序列的研究

Species identification of Mycobacterium tuberculosis with insertion

而其它几株重组病毒拯救不成功，没有检测到特异性的插入序列。

While the viruses containing other foreign sequences were not rescued successfully and the inserted sequence were undetectable .

结核分枝杆菌插入序列6110DNA实时定量检测及其对结核病诊断价值的探讨

Real-time Quantitative Assay of Insertion Sequence 6110 DNA of Mycobacterium Tuberculosis and Its Value for Diagnosis of Tuberculosis

设法用减少插入序列长度的办法，提出一种快速插入的排序方法。

This paper puts forward to a sort method of quick insertion through trying to shorten the length of insertion sequence .

耐药基因可以通过整合子、转座子、插入序列及某些未知的转移机制在质粒间、质粒与染色体间转移或集聚重排。

These resistant genes can be transmitted among plasmids and chromosomes through integron , transposon , IS and other unknown elements .

我们研究发现，有些果蝇基因的外显子和插入序列的碱基组成没有明显区别。

We discovered that there is not an obvious difference between the base components of extrons and intervening sequences of some Drosophila melanogaster genes .

将两株细菌基因组中插入序列和假基因的数量及其分布的对比分析，进一步证实插入序列在假基因形成以及维持细菌基因组多态性的作用。

Compared with the distribution of IS and pseudogenes between genomes of two strains , IS was further demonstrated to play a role at formation of pseudogene and genomic polymorphism .

预测发现该菌株具有大量的插入序列、串联重复序列和长末端重复序列，其中插入序列的存在暗示该菌株在进化过程中发生了大规模的基因片段插入与缺失。

A lot of repeat sequences , including insert sequences , tandem repeat sequences and long terminal repeat , were predicted , which might be the result of adaption to new environment or the insertion and deletion of gene on a large scale .

插入CpG序列和IL-2基因的HBV重组真核表达载体的构建

Construction of recombinant HBV mammalian expression vectors by insertion of CpG motif and IL-2 gene respectively