接触抑制

因此，寻找一种更能够耐受接触抑制的MSCs，应用于细胞治疗，从而提高细胞治疗的效果是必要的。

Therefore , it is needed to find a suitable source of MSCs possessing more tolerance to the contact inhibition for improving the cell therapy .

同时将mtLMP1和wtLMP1分别导入Rat1细胞，接触抑制试验比较二者对细胞的转化作用。

Moreover , mt-LMP1 and wt-LMP1 were transfected into Rat-1 cells to compare their transforming effects by contact inhibition assay .

血清饥饿、接触抑制及生长期细胞对体细胞克隆胚的发育没有显著的影（P0.05）。

There was no significant difference in developmental competence of somatic cell cloned embryos when donor cells were treated by serum starvation , contact restrain , or no-treatment ( P0.05 ) . 9 .

结论AF浓度>4×10-4u/mL，能通过持续接触抑制体外培养的胎儿晶状体上皮细胞的生长，并且具有浓度依赖效应。

Conclusion AF can inhibit the growth of fetal LECs through constant contact when its concentration exceeds 4 × 10 ~ - 4 u / mL , and the inhibitory effect of AF is dose-dependent .

然而，由于移植区域恶劣环境（如：局部缺血、氧化应激、炎症反应等）的作用以及一次性植入大量细胞产生细胞接触抑制而导致MSCs大量的凋亡或死亡，从而降低了细胞治疗效能。

However , bad transplanted environment ( such as ischemia , oxidative stress , inflammatory reaction ) and contact inhibition , which cause abundant death or apoptosis of MSCs , are unfavorable for application of cell therapy .

利用血清饥饿以及接触抑制对细胞进行细胞周期同期化处理，发现细胞同期到G0/G1期的效率相同，而且在处理2d之后，G0/G1期细胞比例不再有显著增加。

Cell cycle stage was synchronized by either serum starvation or contact inhibition . No significant difference of population at G0 / G 1 between them and no further increases of G0 / G1 cell population after extension of synchronization beyond 2d .

结果被LMP1基因转染的细胞生长旺盛，丧失接触抑制，生长速度增快，在软琼脂中能够形成多个集落，并能在裸鼠体内成瘤。

Results The LMP1 gene transfected cells grew vigorously and rapidly , lost contact inhibition , formed more colonies in soft agar , developed tumors in nude mice .

结果培养液的pH值在7.2～7.4之间时，MSCs生长活跃，增殖速度极快，无接触抑制，镜下细胞呈单一梭形外观的成纤维细胞，形成成纤维细胞集落。

Results The growth and the proliferation velocity of MSCs were active and fast respectively when the pH level of culture medium were between 7.2 ~ 7.4 , MSCs formed the cell clone having the anagogic cell shape and exhibited a typical fibroblast-like morphology .

表明微囊囊膜的存在并未对具有贴壁生长和接触抑制性特征的HL-7702细胞的增殖和生理功能产生明显的不利影响。

That the presence of microcapsule membrane did not have adherent contact inhibition of growth and characteristics of the HL-7702 cell proliferation and biological function , significant adverse effects .

培养15～18d，细胞出现80%～90%的融合，克隆间出现重叠，无接触抑制现象发生。

Culture 15 ~ 18d , 80 % ~ 90 % cells emerged fusion , the cells overlapped between clones , and have no the phenomenon of contacting control .

处于同一代龄的细胞，则接触抑制期细胞的ACPase活性显著高于生长期细胞。

In the same PDL group , ACPase ac ivity was much higher in cells of contact inhibition state than that in cells of growth state . Centrophenoxine reduced the ACPase activity in all tested PDLs .

接触抑制的上皮细胞形态不均一。

Appearance of the contact repressing cells are not alike .

细胞生长呈单层，锚着依赖和接触抑制；

The cells remained monolayer , anchorage dependent and the growth was attachment-inhibited .

截肢伤小鼠巨噬细胞对T细胞的直接接触抑制作用

Study on inhibition of T cells by direct contact with macrophages after amputation injury

这些细胞为高度不规则形，重叠生长方式，没有接触抑制；

These cells demonstrated high irregular cell and overlapping growth pattern , without contact inhabit .

结果细胞培养呈单层，锚定依赖和接触抑制生长。

Results At the20th passage , the SHEE cell remained monolayer , anchorage-dependent and attachment-inhibited growth .

该细胞贴壁生长，具有接触抑制的性质。

These cells were able to grow adhering to the wall and stop growing with contact inhibition .

细胞呈贴壁生长，趋向复层，无接触抑制。

The cell line grew with adherence , showing a tendency of multi layer growth without contact inhibition .

培养细胞在相差显微镜下观察，呈典型的铺路石状排列，细胞间存在接触抑制；

The culture cells are observed with microscopy phase contrast and the cells arrange in shape of typical " cobble-stone " .

电镜显示，体外细胞在材料表面及孔隙中附着、生长良好，存在接触抑制现象；

Good adhesion , growth and inhibition of osteoblasts were seen on the surface and pore of the material in vitro .

A组细胞随培养时间的延长细胞呈多层生长，不发生接触抑制现象。

The cells in the Group A grew with many layers along with the prolongation of the culture time without contacted inhibition .

创伤后巨噬细胞对T细胞白介素2及白介素2受体α基因表达的直接接触抑制作用

Inhibition of T cells interleukin 2 and inter - leukin 2 receptor a gene expression by direct contact with macrophages after trauma

所建北山羊和山羊成纤维细胞系开始生长缓慢、后期生长接触抑制，属于正常细胞；

The cell lines of goat and Capra ibex were normal cells because they grown slowly at beginning and then had contact inhibition .

利用小鼠截肢伤模型，观察创伤小鼠巨噬细胞对T细胞的直接接触抑制作用。

A murine amputation injury model was used and the inhibition of T cells by direct contact with macrophages from traumatized mice was observed .

对以上结果和视黄酸及亚硒酸钠使肝癌细胞接触抑制恢复及表型逆转的关系作了讨论。

The relationship of above mentioned results to the restoration of contact inhibition and the reverse of the phenotype of the hepatocarcinoma cell was discussed .

从转化灶中分离的细胞，失去了在培养中的接触抑制，能在软琼脂培养基中形成集落。

The transformed cells separated from the foci lost their ability of post-confluence inhibition of division , and could grow into colony in soft agar medium .

另外，黏附丧失可导致接触抑制障碍，从而在肿瘤发生的早期阶段起某种作用。

In addition , loss of cell adhesion may contribute to loss of contact inhibition and thus play a role at the earlier stage of the neoplastic process .

调节T细胞是一类具有免疫抑制作用的细胞群，通过细胞接触抑制或抑制性细胞因子主动抑制自身免疫性T细胞。对于维持机体免疫自稳、预防自身免疫病具有极其重要的作用。

Regulatory T cells ( Treg ) are a kind of cell group with immunosuppressive effects , which inhibit autoimmune T cell by cell-contact suppression or suppressive cytokines .

肝癌细胞在体外二维培养中存在明显的接触抑制丧失现象，突出表现为细胞恶性增殖行为。

Liver cancer cells in vitro two-dimensional cultivation have an obvious phenomenon of loss of contact inhibition , which is the outstanding performance for the proliferation of malignant cells .

5号以短梭形为主，核质比例变小，有一定的接触抑制，致瘤能力较低，为高分化的骨肉瘤细胞株。

The 5th clone strain was high differentiation with more multiangle , low nucleo-cytoplasmic ratio , limited contact inhibition , inferior capacity of tumorigenicity in nude mice and so on .