悬浮细胞

不同诱导处理后水稻悬浮细胞的活性氧变化与有关酶系的关系

Relationship between the Changes of Active Oxygen Species and Defense Enzymes in Suspension Cultured Cells Treated by Different Inducers

这些结果表明Ca~（2+）-CaM参与了机械刺激诱导的烟草悬浮细胞中NO的产生。

All of these results suggest that Ca ~ ( 2 + ) - CaM involved in the MS-induced NO formation in the tobacco suspension cultured cells .

H2O2诱导烟草悬浮细胞的凋亡

H_2O_2 Induces Apoptosis of Tobacco Suspension Cells

高产SOD大蒜悬浮细胞系的选育

Selection of high-yield SOD garlic suspension cell line

水稻悬浮细胞中稻瘟菌激发子诱导性受体类似激酶cDNA的克隆及特征分析

Cloning and characterization of an elicitor inducible receptor like kinase cDNA from rice suspension cells

Rr射线照射使悬浮细胞系产生致死损伤，更加速了细胞嚣解体和细胞死亡。

Treatement with5000R γ - rays brought about even greater damage to the suspensions , accelerating the disintegration of cell organs .

进行了大蒜愈伤组织的诱导以及高产超氧化物歧化酶（SOD）大蒜悬浮细胞系的筛选。

Induction of garlic callus and selection of highly producing SOD garlic suspension cell line were studied .

玉米转座因子Ac／Ds导入水稻花药悬浮细胞并再生成植株

Introduction of Maize Transposable element Ac / Ds into Rice Anther-derived Suspension Cells and Plant Regeneration

机械刺激诱导烟草悬浮细胞NO生成的途径及其与Ca～（2+）/CaM的相关性

Mechanical Stimulation-induced Nitric Oxide Burst , Possible Pathways and Involvement of Ca ~( 2 + ) and CaM in Tobacco Cultured Cells

筛选获得了高产SOD大蒜悬浮细胞系，培养18d后达到最大生物量及最大SOD总酶活分别为22.12g。

High yield SOD garlic suspension cell line was obtained after selection .

以上结果表明cAMP参与了烟草悬浮细胞中ABA信号的传递。

These results suggested that cAMP was an important messenger in ABA signal transduction in tobacco suspension cell .

收集培养至第3周的悬浮细胞，采用免疫细胞化学法进行CD34单克隆抗体鉴定。

The suspended cells were collected after 3 weeks of culture for anti-CD34 monoclonal antibodies identification by immunocytochemistry .

用苹果（MaluspumilaMill）胚珠诱导愈伤组织并建立悬浮细胞系。

Calli were induced and suspension cell lines were set up from ovule of Malus pumila Mill .

结论改良MTT法在研究悬浮细胞增殖及药物毒性方面明显优于传统方法。

Conclusions Improved MTT assay had more advantages than the traditional one in studying the proliferation and cytotoxicity on suspended cell lines .

利用植物组织培养并结合HPLC检测技术，对鬼臼毒素等9种活性化合物在掌叶大黄发状根、离体培养根及悬浮细胞3种培养系统中的生物转化进行了研究。

By using plant tissue culture technology and HPLC techniques , the biotransformation of 9 active chemicals by the 3 in vitro culture systems of Rheum palmatum was studied .

结果表明：在每个7d一次的继代周期中，拟南芥悬浮细胞的生长表现出明显的S型生长曲线。

The typical S-like growth curve in a subculture cycle lasting 7 days was obtained .

抗逆诱导剂IPT和ABA对水稻悬浮细胞PAL、LOX基因表达状况的影响

Effects of Stress-Resistance Inducer IPT and ABA on Expression of PAL 、 LOX Genes in Suspension Cells of Rice

随着愈伤组织继代次数的增加，悬浮细胞胞外POD活性和细胞悬浮度呈先上升后下降趋势。

In the latter generations of rice suspension cell , the activity of extracellular POD and the cell suspensibility curve raises at first and then declines .

研究了甘露醇和6-BA处理对水稻悬浮细胞再分化、过氧化物酶及IAA氧化酶的影响。

Effects of mannitol and 6 BA on the rice cell redifferentiation , activities of peroxidase and IAA oxidase were investigated .

以白桦悬浮细胞为材料，通过同源序列RT-PCR方法结合RACE技术首次克隆获得白桦法呢基焦磷酸合酶FPS的cDNA全长序列。

Birch suspension cells as material , through homologous sequence RT-PCR method combined with RACE technology cloned and obtained firstly birch farnesyl pyrophosphate synthase FPS cDNA full-length sequence .

4℃低温2h处理能促进悬浮细胞生长，提高植板率；

Low temperature treatment at 4 ℃ for 2 hours could enhance the growth of cell , and increase the planting efficiency as well .

3天后更换培养液，除去绝大部分悬浮细胞，可见分散的BMSCs小集落，集落细胞呈放射状生长。

The culture medium was changed after days . Remove most of the suspended cells , we can found scattered BMSCs colony , radially grew .

CEV可以在悬浮细胞中复制。

CEV cln replicate in cell suspension cultures .

结果：诱导5～7d后的悬浮细胞具有典型的树突状细胞形态。

Results : After 5 ~ 7 days ' culture , the non-adherent cells had the typical morphology of DCs .

贴壁24h后去悬浮细胞、低接种密度传代培养可获增殖能力强、功能活跃的MSCs。

Discarding the suspending cells after 24h incubation and subculturing the cells with low seeding density were helpful for acquiring MSCs with improved reproductive capability and active function .

培养48h的粘附和悬浮细胞之PO活性均低于培养24h的相同细胞（P<0.01）。

The PO activity of the adherent cells and detached cells cultured 48 hours were lower than the same cells cultured 24 hours ( P < 0.01 ) .

本文报导在美国从普度大学校园的红豆杉属（Taxus）植物的嫩针叶和嫩茎枝诱导出愈伤组织（Callus）和建立悬浮细胞培养。

In this paper , the callus was induced by young needles and young stems of Taxus at Purdue University Campus . And the cell suspension culture of Taxus was established .

在WP培养基中补充营养物质可使悬浮细胞中的总生物碱达最高水平（345.33mg/l）。

The highest level of total alkaloid ( 345.33mg / I ) has been obtained when nutritious materials has been replenished in the WP medium .

免疫细胞化学染色结果显示，培养至两三周后的骨髓悬浮细胞多数呈CD34阳性，阳性率达（74.6±1.7）%。

The result of immunohistochemistry staining suggested that most of the suspended cells cultured for 2 to 3 weeks showed CD34-positive with positive rate of ( 74.6 ± 1.7 ) % .

用ABA处理悬浮细胞24h后GUS活性显著升高，说明外源ABA能够诱导rd29A启动子的表达，获得了用于ABA信号转导研究的实用细胞系。

Treatment of the suspension cells with ABA led to an increase in GUS activity , indicating that these transgenic suspension cells are useful for the study of ABA signaling .