延伸反应

引物延伸反应表明DNA分子发生聚合。

The primer extension experiment showed the aggregation of DNA template by photooxydation .

基于引物延伸反应进行SNP基因分型的电化学方法

Electrochemical Detection for SNP Genotyping Based on Primer Extension Reaction

应用建立的HPLC方法检测单碱基引物延伸反应的产物，并且对产物进行了定量。

The products of SNuPE for2 Y-SNP loci were qualified and quantified with the ion-pair reversed-phase HPLC .

利用两种基因的特定引物及总RNA进行的引物延伸反应产物在电泳的凝胶上均呈现一条十分清楚的带，表明这两种多拷贝小麦基因的转录分别起始于一个核苷酸。

One distinct band was detected in each primer extension reaction with total RNA extracted from wheat leaves and the primers specific for the wheat genes .

长、短链探针以链延伸反应提高基因传感器检测MRSA灵敏度的实验研究

Sensitivity Improvement of Detecting MRSA Using Gene Sensor by Strand Extension Reaction with Long and Short Chain Probes

RT-PCR扩增出VH和VL基因，经重叠延伸反应组装成单链抗体（ScFv）。

The VH and VL genes were amplified by RT-PCR , and ScFv was assembled by splicing overlap extension PCR .

MALDI-TOF-MS结合引物延伸反应检测K-ras基因型方法的建立

Establishment and Application of Research Model for Screening the K-ras Alleles by MALDI-TOF-MS Coupled with Primer Extension Reaction Method

最后在72℃延伸反应10min。

And 5 min final extension at 72 ℃ .

目的利用链延伸反应的原理延长引物链以提高石英谐振式基因传感器表面的质量负载，从而提高传感器检测MRSA的灵敏度。

OBJECTIVE To improve the sensitivity of detecting MRSA using quartz oscillation gene sensor ( QOGS ) by increasing its surface load on the basis of the principle of strand extension reaction .

方法应用荧光标记双脱氧核苷酸进行单碱基延伸反应，同步检测人类DRB基因10个单核苷酸多态性位点。

Methods Using fluorescent dye labeled ddNTP , the single nucleotide extension ( SNE ) reaction was conducted to analyze 10 SNP loci in human DRB gene .

方法采用GP5+/GP6+引物系统扩增58例宫颈癌组织，将荧光偏振（FP）检测技术与模板指导的末端延伸反应（TDIFP）结合，检测HPV58，确定HPV58感染阳性宫颈癌组织。

Methods HPV 58 from 58 biopsy tissues of cervical cancer was detected by GP5 + / GP6 + PCR followed by template-directed dye-terminator incorporation assay with fluorescence polarization detection ( TDI-FP ) .

基因传感器阵列表面分别固定上金黄色葡萄球菌mecA及femA两种探针片段，待杂交上相应的靶序列片段后，加入Klenow酶在室温下进行链延伸反应。

Two kinds of mecA and femA probes of staphylococcus aureus were immobilized on the gold electrode surface of QOGS array . Klenow large fragment was added to extend the strand in room temperature after the probes were hybridized with corresponding target DNA . 4 .

链延伸反应提高压电基因传感器的灵敏度的实验研究

Sensitivity improvement of piezoelectric gene sensor by strand extension reaction

末端标记引物延伸反应对核酸单碱基辨认的实验研究

Single Base Discrimination Using Terminal Labeled Primer Extension

该方法是基于由目标分子引发的等温指数降解反应，即结合了聚合酶的链延伸反应和限制性内切酶的双链切割反应。

The method is based on a novel target triggered isothermal exponential degradation reaction ( TT-isoTexpDR ), which integrates polymerase strand extension and double strand cleavage .

建立用普通高效液相色谱仪检测单链寡聚核苷酸的方法，为检测单碱基引物延伸反应的产物提供定量分析基础。

To quantify the products of single nucleotide primer extension ( SNuPE ), the single strain oligonucleotides were separated by High Performance Liquid Chromatography ( HPLC ) .

修饰引物的3′端第三个碱基处人为导入一个错配碱基来改善引物延伸反应的开关特性。

The switching characteristics of the primer extension reaction was increased by introducing an artificially mismatched base into the third position from the 3 ′ - terminus of the primers .

基于单碱基延伸标签反应的常见食源性致病菌基因芯片检测方法的建立

Development of Single Base Extension-tags Microarray for the Detection of Food-borne Pathogens

应用引物延伸预扩增反应技术检测孕妇血浆中的胎儿DNA

Detection of fetal DNA using primer extension preamplification followed by nested PCR

KCS基因家族编码β-酮脂酰CoA合成酶，催化超长链脂肪酸延伸过程的缩合反应，是超长链脂肪酸合成的限速酶。

KCS gene family encode β - ketoacyl CoA synthase , a rate-limiting enzyme in the synthesis of very long chain fatty acid ( VLCFA ), catalyzing the condensation reaction of VLCFA elongation process .