鸭瘟

用生物素标记核酸探针检测鸭瘟病毒DNA的研究

Detection of duck plague virus DNA with a biotin - labelled probe

鸭瘟病毒PCR检测方法的建立及验证

Development and Verification of PCR for Detection of Duck Plague Virus

鸭瘟病毒感染及其诱导α干扰素mRNA水平的动态定量研究

Quantitative Study on Duck Plague Virus Infection and Alpha-interferon mRNA Level

利用PCR检测鸭瘟病毒

Detection of duck plague virus by PCR

鹅细小病毒和鸭瘟病毒复合PCR检测方法的建立

Development of a multiplex PCR assay for detection of goose parvovirus and duck plague virus

鸭瘟病毒UL26基因原核表达及宿主细胞中的定位研究

DPV U_L26 Gene Prokaryotic Expression and the Study of Its Cellular Localization in Virus-infected Host Cells

鸭瘟病毒gB基因B细胞和T细胞表位的预测及其主要抗原域基因的原核表达

Prediction of B-and T-cell epitopes of gB gene of duck plague virus and prokaryotic expression of its major antigen determinants

试验结果表明，生物素标记核酸探针可检出10pg提纯的鸭瘟病毒DNA，并检出稀释10~5倍和肝组织鸭瘟病毒DNA；

The results showed that the biotinylated DNA probe could detect purified homologous DNA target at 10 pg level and could detect DPV DNA in 10 ~ 5-fold dilution of the liver tissue .

皮下注射鸭瘟弱毒苗诱导鸭局部黏膜和系统免疫中IgA、IgM和IgG的发生规律

Regularity of IgA , IgM and IgG antibodies in local mucosal and systemic immunity of ducklings vaccinated subcutaneously with attenuated duck plague virus vaccine strain

鸭瘟弱毒Cha株不同途径免疫雏鸭在体内分布和排泄规律的研究

Study on the Distribution and Excretion in Ducklings Vaccined with Duck Plague Virus Attenuated Cha Strain in Different Inoculated Routes

以DPV标准强株DNA为模板，建立了实时荧光定量PCR检测鸭瘟病毒的方法。

The primers amplified a 101 bp fragment between the sites 891-991.With DNA obtained from standard virulent strain of DPV , a real-time quantitative PCR for DPV was established successfully .

运用生物信息学方法，对鸭瘟病毒TK基因的基本元件和TK蛋白的理化性质、结构、功能进行分析。

The basic elements of Duck Plague Virus TK gene and physicochemical properties , structure , function of TK protein were analyzed by using bioinformatics method .

将鸭瘟病毒分离株SD-01在鸡胚成纤维细胞上增殖。

The SD-01 strain of DPV was propagated in chicken embryo fibroblast monolayer cells .

结果表明该方法可应用于临床鸭瘟的诊断与检测和DPV强毒株和疫苗株的PCR鉴别诊断。

The results showed that this PCR method based on DPV dUTPase gene can be applied in the diagnosis and detection of DPV , and it could also be used in differential diagnosis of DPV virulent strain and attenuated strain .

鸭瘟是由鸭瘟病毒（DPV）引起鸭和鹅的一种急性接触性传染病。不同年龄和品种的鸭均可感染该病毒，以麻鸭、番鸭和绵鸭感染性最高。

Duck plague which is caused by duck plague virus ( DPV ) is an acute contagious disease of ducks and goslings , No matter what age group or variety can infect this virus .

依据文献报道的鸭瘟病毒（DPV）的核苷酸序列，设计和合成了二对引物，分别为SP1和SP2，LP1和LP2。

According to the sequences of Duck Plague Virus ( DPV ) from GenBank , two pairs of primer , SP1 and SP2 , LP1 and LP2 , were designed .

鸭瘟病毒（DPV）为一种α疱疹病毒，是鸭瘟（DP）的病原。鸭瘟是鸭、鹅和天鹅等水禽的急性、高度接触性传染病，给养禽业造成了巨大的经济损失。

Duck plague virus ( DPV ), an alphaherpesvirus , is the causative agent of duck plague ( DP ) that is an acute , contagious disease in ducks , goose , swans and other waterfowls . DP has been responsible for considerable economic losses to the commercial duck industry .

商品肉鸭鸭瘟病毒的分离与鉴定

Isolation and identification of duck plague viruses from commercial meat-type ducks

这是国内外对鸭瘟病毒以上基因序列的首次报道。

This is the first report of the DPV genes above .

鸭瘟抗体检测方法研究

Detection of Antibodies against Duck Plague Virus by ELISA

鸭瘟病毒提纯方法比较

Comparison of purification methods of duck plague virus

鸭瘟病毒基因组核酸制备

Preparation of Genome DNA of Duck Plague Virus

雏鹅感染鸭瘟的诊断及防治

Diagnosis and Prevention of Duck Plague in Goslings

该结果有利于对鸭瘟病毒的诊断及致病机理的研究。

The results will help us to study the diagnosis and pathogenic mechanism of DPV .

不同条件对鸭瘟病毒感染细胞蛋白质组二维电泳分析结果的影响

Two-dimensional gel electrophoresis model for proteomic analysis of duck plague virus infected duck embryo fibroblasts

雏鹅的鸭瘟病原分离鉴定及油乳剂灭活苗研制

Isolation and Identification of Duck Plague Pathogens in Goslings and Development of An Oil-emulsion Inactivated Vaccine

鸭瘟病毒的研究

Investigations on duck plague virus

暴发性鸭瘟的诊断

Diagnosis of fulminant duck plague

鸭瘟病毒部分基因组序列的克隆及重组病毒转移载体的构建

Cloning and Analysis of Partial Genomic Sequence of Duck Enteritis Virus and Construction of Transfer Vector for Recombinant Virus

一种新型鸭瘟病原的分离鉴定及其特性鸭新型疱疹病毒的致病性研究

Isolation and Identification and Characteristics of the Pathogen of a New Duck Plague Pathogenicity of New Type Herpesvirus in Duck