幼嫩

利用青花菜幼嫩雌蕊进行染色体核型分析

Broccoli Karyotype Analysis by Using Immature Pistil

各个品种幼嫩豆荚的大小、形状、色泽、纤维素多少和嫩软程度差别很大。

Varieties differ greatly in size , shape , colour , and tenderness of the immature pods .

这些幼嫩的植物需要精心培育。

These delicate plants need careful nurturing .

但这真的让我很不舒服,因为它们在我幼嫩的皮肤上感觉很粗糙。

But it really made me uncomfortable , for they felt rough against my young skin .

结果表明：从幼嫩的叶片中提取的DNA质量高；

The results showed that the highest quality of DNA was from tender leaves .

基因表达分析表明：叶绿体Cu/ZnSOD基因在幼嫩的茎、花中有微量的表达，在叶片中高效表达；

The expression of chloroplast Cu / Zn SOD gene was determined using northern blotting analysis .

对T1代转基因植株的RTPCR分析表明，两个外源抗病基因在衰老叶片中的表达强于幼嫩叶片。

RT-PCR performance with T 1 plants showed that the transgenes express strongly in senescence leaves .

以大豆、黄瓜、水稻、玉米的幼嫩叶片为材料，采用4种不同的DNA提取方法，比较不同方法的DNA提取效率。

Compare the efficiency of 4 various DNA extraction methods on different plants , using the young leaves of soybean , cucumber , rice and maize as materials .

方法：以龙胆草幼嫩的茎为外植体，培养在MS培养基上，通过胚状体途径获得龙胆草组培苗芽丛。

Method : Tender stems of Gentiana scabra Bunge were cultured as explant in MS medium and clump of buds were acquired via embryoid .

愈伤组织、外植体的幼嫩叶片、花瓣在含不同浓度BA培养基上均未能诱导产生芽；

The bud can 't be induced from callus , petals and spires of explant with various concentration of BA ;

GUS的活性在幼嫩叶片中要高于衰老叶片中。

Moreover , the GUS activity was stronger in young leaves than in old leaves .

说明CPT的积累趋向于植物幼嫩和生长旺盛的组织。

All the facts explain that CPT tends to be accumulated in younger and actively growing tissues .

根据总RNA完整性、纯度和得率筛选出适合多花蔷薇幼嫩根、叶总RNA的提取方法。

The isolation procedure of total RNA from young root and leaf tissue of Rosa multiflora was optimized according to the integrity , purity and yield of RNA .

用强诱变剂N-甲基-N-硝基-N-亚硝基胍（NG）处理条斑紫菜和坛紫菜的幼嫩叶状体，进行色素突变体诱导。

: The young thalli of Porphyra yezoensis and P. haitanensis are induced by N-Methy-N-Nitro-N-Nitrosoguanidine ( NG ), a strong mutagen to induce the mutant of chromatophore .

EBL对PAs的调控最为明显的部位也是较幼嫩的叶片和靠近根尖的区域。

The most obvious places regulated by EBL were also more tender leaves and near the apical region of roots .

将柽柳幼嫩枝条接种于诱导培养基上诱导出丛生芽，再把柽柳丛生芽和嫩枝接种在含有不同浓度NAA的MS培养基上，使外植体分化生根。

Young branches of Tamarix chinensis were inoculated in MS medium for cluster bud , then these buds and young branches were transferred to MS medium supplemented by different concentration of NAA for root induction .

以蓝莓幼嫩叶片为试材，采用CTAB法提取基因组DNA，并对影响RAPD反应的因素进行了研究，建立了适用于蓝莓的RAPD最佳反应体系。

By CTAB method the genomic DNA of blueberry was extracted , using tender leaves as materials . Several factors which affected RAPD amplification in blueberry genomic DNA were analyzed and a better system was promoted .

4h后，叶片脱落酸含量也显著增加，从最幼嫩叶片到成熟叶片分别达到处理前的25，26，17，10和6倍。

Four hours after stress , ABA contents in leaves increased 25 , 26 , 17 , 10 , and 6 times respectively from the youngest leaves to mature leaves .

以虎舌红（ArdisiaMamillataHance）幼嫩茎尖为外植体，采用正交设计及方差分析对影响虎舌红植株离体培养的因素进行了优化研究。

The influencing factors of Ardisia mamillata Hance in vitro cultivation were optimized by orthogonal design and variance analysis using tender stem apexes of A.

SCN的二龄幼虫侵入到大豆幼嫩的根组织中，导致大豆根内的细胞变形并与之形成合胞体。

The second-stage juvenile ( J2 ) of SCN penetrates soybean roots and causes the formation of specialized feeding cells which are usually called syncytium in the roots ' vascular system .

以麻疯树幼嫩枝条腋芽为外植体，以MS为基本培养基，附加不同浓度的激素组合（6BA与IBA）进行芽诱导和增殖培养。

The axillary bud on young shoot of Barbados nut was used as explants , adding different concentration of plant hormone combinations ( 6-BA and IBA ) on minimal medium ( MS ), to carry out buds inducing and multiplication culture .

其原因一方面是由于不同组织运输IAA的能力不同，老组织（基部）运输IAA的能力低于幼嫩组织（上部），节部组织运输IAA的能力低于节间组织。

One reason might be because of lower capacity of the old tissue ( base ) to transport 3 H IAA than the young tissue ( upper part ) and lower capacity of node to transport 3 H IAA than internode .

通过试验不同激素配比研究发现：大多数以川芎幼嫩茎段为外植体均能长出愈伤组织，向培养基中添加6-BA（0.4mg/L）和2,4-D（2.0mg/L）为最佳，愈伤组织诱导率达87.5%；

Fresh shoots of Ligusticum chuanxiong Hort were used as explants in the culture tissue . The different harmone proportions with 6-BA and 2,4-D as hormones to induce callus and 6-BA , IAA to induce buds differentiating were comparison in detail .

该体系是以1cm2左右带叶脉的幼嫩叶切片为外植体；

The results showed that petiole Rieger begonia leaf with main vein ( 1 cm2 ) was used as explants ;

从渗透调节能力和离子稳态调控方面来看，EBL都是优先保护盐胁迫植株的幼嫩组织和生殖器官，这能大大提高植物的盐渍适应能力。

In view of regulations of osmotic adjustment ability and ion balance , EBL was priority to protect young tissues and reproductive organs of the salt-stressed plants , which could greatly increase the adaptation of plants to salinity .

全明星以2000Lx光强下培养的30～35d龄左右继代试管苗上幼嫩的，三片小叶都伸展开的叶片再生效果最好。

For " Allstar ", newly and fully unfolded young leaves from subculture shoots cultured for 30 to 35 days under 2000 Lx light intensity were most responsive to regeneration .

2,4D和6BA浓度明显影响愈伤组织质量和诱导率，幼嫩幼穗和较小的未成熟胚愈伤组织诱导率高。

The induction frequency and quality of calli were significantly influenced by the concentrations of 2,4-D and 6-BA . Young ears and smaller immature embryos produced calli in higher frequency .

FAD2-2A基因在根和发育中期、后期的种子中低水平表达，在幼嫩的茎、叶以及发育早期的种子中高水平表达；

FAD2-2A transcript showed a low level in the root , middle-stage seeds and pre-mature seeds , and FAD2-2A was expressed more in the first-stage seeds and tender leaves than in the other tissues .

FAD2-1A基因在根中的表达量最低，在种子发育早期、中期大量表达，在幼嫩叶子中表达水平也较高；

FAD2-1A transcripts showed the lowest level in the root and the highest level in the first-stage and middle-stage seeds , and it was expressed abundantly in the tender leaves , too .

三种植物幼嫩花粉原生质体的分离与培养

Isolation and Culture of Young Pollen Protoplasts in Three Plant Species