巢蛋白

缺氧条件下鼠胚软骨c-Fos和巢蛋白表达及当归的保护作用

Effect of Chinese angelica on the expression of c-Fos and nidogen in embryonic cartilage of rats during hypoxia stage

应用Olympus光学显微镜观察细胞抗-神经巢蛋白和D2受体阳性染色阳性细胞数目计数。

The number of positive cells of cell anti-nerve nidogen and D2 receptor positive staining with Olympus optical microscope .

巢蛋白mRNA在小鼠中枢神经系统发育过程中的表达

Nestin mRNA expression during the development of mouse central nervous system

慢性压迫性脊髓损伤后巢蛋白mRNA表达的观察

Nestin mRNA expression after chronic compressive spinal cord injury in adult rats

与小鼠cDNA序列比较结果表明，小鼠巢蛋白基因包含3个内含子。

Sequence comparison of the cDNA and genomic DNA revealed three introns in this gene .

c-Fos和巢蛋白在鼠胚肺内的表达及缺氧对其影响

Expression of c-Fos and nestin in lung of rats ' embryo and effect of hypoxia

免疫荧光染色检测神经干细胞特异性抗原神经巢蛋白Nestin的表达；

Expression of the neural stem cell marker nestin by immunoflurescence staining ;

目的探讨巢蛋白（nestin）在瘢痕疙瘩和增生性瘢痕中的表达。

Objective To investigate the expression of nestin in keloid and hypertrophic scarring .

目的观察成年大鼠慢性压迫性脊髓损伤后及减压后早期巢蛋白与巢蛋白mRNA的相关性表达。

Objective To investigate the regeneration of neural precursor cells in adult rats after chronic compressive spinal cord injury by analyzing the expressions of nestin and nestin mRNA .

双重反应结果显示，巢蛋白与GABA在海马神经元内无共存。

Double labeling immunohistochemistry demonstrated that there was no co existence of nestin and GABA within the hippocampal neurons .

克隆细胞表达巢蛋白（Nestin）。

The clones expressed nestin antigen .

部分胰岛素和CK19染色强阳性的细胞中巢蛋白染色为弱阳性，胰升糖素强阳性的部位则未见巢蛋白染色阳性的细胞。

Some of the insulin and CK19 positively staining cells were positive for nestin expression .

在成年SD大鼠的脑室系统中，巢蛋白主要分布在侧脑室外侧壁及其周围脑区。

In the CNS of adult SD rats , however , nestin protein was mainly located in the lateral wall of the lateral ventricle and the adjacent region .

目的通过检测胃肠道间质瘤（GIST）组织中巢蛋白（nestin）的表达，探讨nestin在GIST的诊断、鉴别诊断及起源中的意义。

Objective To investigate the expression of nestin protein and its clinicopathological and diagnostic significance in gastrointestinal stromal tumor ( GIST ) .

倒置显微镜下观察NSCs形态及生物学特性，免疫细胞化学方法（SABC法）检测巢蛋白（Nestin）。

Observing the biological characteristics of NSCs under the inverted microscope morphological and immunocytochemistry ( SABC ) detect nestin .

小鼠巢蛋白（nestin）基因编码了一个中等纤维骨架蛋白，该基因在小鼠中枢神经系统发育过程中瞬时性表达。

Abstract Mouse nestin , an intermediate filament gene , is transiently expressed during the development of the central nervous system .

诱导6d，部分细胞有神经巢蛋白成分表达。

Some cells had the expression of the component of nerve nidogen after induction for 6 days .

目的：观察巢蛋白（nestin）在局灶脑缺血区的反应模式，以探讨其在脑梗塞灶修复过程中的作用。

AIM : To explore the expressive profile of nestin protein in the focal ischemic brain and to study the recovery mechanism of brain focal infarct .

结果体外培养的神经球表达巢蛋白（nestin），神经球分化的细胞表达神经丝和胶质纤维酸性蛋白。

Results Neural spheres express nestin and the cells differentiated from the spheres express neurofilament 200 ( NF200 ) or glial fibrillary acidic protein ( GFAP ) .

巢蛋白和阶段特异性胚胎抗原-1在O-2A谱系细胞中的表达

The Expression of Nestin and Stage Specific Embryonic Antigens-1 in O-2A Lineage Cells

以免疫组化方法检测神经元特异性烯醇化酶（NSE）、神经丝蛋白（NF-M）、巢蛋白（nestin）、胶质纤维酸性蛋白（GFAP）的表达。

Neuron-specific enolase ( NSE ), neurofilament ( NF ), nestin , glial fibrillary acidic protein ( GFAP ) were detected by immunocytochemistry .

方法应用动脉瘤夹压迫建立大鼠脊髓压迫损伤模型，通过组织病理学及免疫组织化学方法检测不同时段室管膜细胞的反应性增生和神经外胚层多潜能细胞特异性抗原巢蛋白（nestin）的表达。

Methods Rat spinal cord injury models were established by aneurysm clip compression , nestin expression and cell proliferation of ependymal cells were showed with pathological and immunohistochemical staining .

模型组和神经生长因子治疗组3d时缺血皮质巢蛋白阳性突起主要与胶质纤维酸性蛋白共存，14d时巢蛋白与神经元特异性烯醇化酶共存明显增多。

In the model and NGF treatment group , nestin positive cells mostly coexisted with glial fibrillary acidic protein on the 3rd day , and double stained with neurone specific enolase was obviously increased on the 14th day .

目的研究磁刺激对损伤脊髓组织的中间丝蛋白&巢蛋白（nestin）表达的影响。

Objective To study the effects of magnetic stimulation ( MS ) on expression of intermediate filament protein ( nestin ) in spinal neurons of rats after spinal cord injury ( SCI ) .

巢蛋白抗体IgG，β-微管蛋白Ⅲ抗体IgG，胶质纤维酸性蛋白抗体IgG，生物素标记二抗和三抗、异硫氰酸荧光素标记二抗。

Nestin antibody IgG , β - microtubule protein ⅲ antibody IgG , glial fibrillary acidic protein antibody IgG , biotin labeled second antibody and third antibody and fluorescinisothiocyate ( FITC ) - labeled second antibody were used in this experiment .

未诱导细胞近100%呈巢蛋白阳性，仅有极少数细胞Tubulin阳性或胶质纤维酸性蛋白阳性。

About 100 % of the cells that were not induced appeared positive nidogen , only extremely a few cells had Tubulin positive or positive expression of glial fibriliary acidic protein .

用免疫组织化学和逆转录-聚合酶链反应（RT-PCR）方法检测巢蛋白、胰十二指肠同源盒基因-1（Pancreaticandduodenalhomeoboxgene-1，PDX-1/IPF-1）以及胰岛内分泌激素胰岛素和胰升糖素（Glucagon）的表达。

Immunohistochemical staining and reverse transcription-polymerase chain reaction ( RT-PCR ) were used to detect the expression of the nestin , pancreatic and duodenal homeobox gene-1 ( PDX-1 / IPF-1 ), islet endocrine hormone insulin and glucagon .

目的研究电针治疗对成年大鼠脑缺血后缺血侧神经干细胞巢蛋白（nestin）表达的影响，探讨电针治疗缺血性脑损伤的作用机制。

Objective To study the influence of electroacupuncture on the expression of Nestin in neural stem cells after focal cerebral ischemia in rats and to explore the mechanism of electroacupuncture in treating ischemic brain injury .

RPCR结果显示全反式视黄酸诱导组巢蛋白在诱导前后均有表达，神经元特异性烯醇化酶在诱导后16h可见明显的扩增条带，24h后更加明显。

RT-PCR results showed that atRA group expressed nestin before and after induction . At 16 hours following induction , NSE exhibited significant amplification band , and became more obvious at 24 hours .

将从2个月龄大鼠脑海马、纹状体等区域分离的细胞培养于含EGF和bFGF的DMEM/F12培养液中，在光镜下观察细胞的形态特征，并行神经巢蛋白（nestin）细胞化学染色。

NSC isolated from two months old rat ′ s brain region like hippocampus and striatum was cultivated in a DMEM / F12 medium containing EGF and bFGF , and was identified with morphological character and nestin immunocytochemistry test .