嵌合基因

NOS/HuIFNa嵌合基因的构建及其与Ti质粒载体的拼接

Construction of nos / huifna chimeric gene and its fusion with Ti plasmid vector

IL-18和HIV-1gag-gp120嵌合基因DNA疫苗联合免疫小鼠的免疫应答

The immune response in mice inoculated with HIV-1 gag-gp120 chimeric gene DNA vaccine and IL-18 plasmid

两种人粒酶B嵌合基因的表达及对肿瘤细胞的杀伤作用

Expression of Two Chimeric Human Granzyme B Genes and Their Killing Activities of Tumor Cells

bax嵌合基因克隆及其在人舌癌细胞的表达

Clone of bax chimeric gene and its expression in human squamous lingual carcinoma cell line

结果：bax基因编码序列及嵌合基因限制性酶谱正确；

Results : The coding sequence of bax and pSV-CIP-bax-CAT gene was characterized by restriction analysis .

P（SAG12）-ipt嵌合基因转化樱桃番茄的研究

Transference of the Gomphosis Gene P_ ( SAG12 ) - ipt Into Cherry Tomato

目的：构建能在人舌癌细胞中高效表达Bax蛋白的bax嵌合基因。

Purpose : To construct a bax chimeric gene that can be expressed effectively in human squamous lingual carcinoma cell line .

嵌合基因bcr-abl表达融合蛋白Bcr-Abl。

This fusion gene encodes chimeric protein Bcr - Abl .

番茄ACC合成酶基因的反义RNA-核酶嵌合基因植物表达载体的构建及对番茄的转化

Construction of an Expressing Vector for Antisense RNA-Ribozyme Chimeric DNA Sequence Against Tomato ACC Synthase Gene and Transformation of Tomato

P（SAG12）序列克隆及P（SAG12）-ipt嵌合基因植物表达载体的构建

Cloning and Sequence Analysis of P_ ( SAG12 ) and Construction of Plant Expression Vector with the Chimeric Gene of P_ ( SAG12 ) - ipt

目的：探讨IL18和HIV1gaggp120嵌合基因的DNA疫苗联合免疫小鼠的免疫应答。

AIM : To explore the immune response in mice inoculated with HIV-1 chimeric gene gag-gp120 DNA vaccine and IL-18 plasmid .

目的构建表达HIV1gaggp120嵌合基因的酵母工程菌，并优化表达条件。

Objective To make up an engineering yeast strain expressing HIV 1 gag gp120 chimeric gene .

目的：在本室前期工作的基础上构建汉滩病毒M基因G2片段与S基因0.7kb片段嵌合基因的重组腺病毒。

AIM : To express the chimeric gene containing M segment and 0.7 kb fragment of S segment of Hantaan virus in adenovirus expression system .

结论：诱导剂佛波酯和BCR-ABL嵌合基因可以影响EDAG基因在白血病细胞系K562中的表达。

CONCLUSION : PMA and BCR-ABL chimeric gene can influence the expression of EDAG gene in leukemia cell line K562 .

VCH3（-276）GUS（无SA顺式作用元件）嵌合基因驱动的GUS酶活性最低，仅仅稍高于对照。

VCH3 ( - 276 ) GUS ( not containing SA cis-motif ) directed the lowest GUS activity , only a little higher than CK .

本文从植物线粒体基因组结构特点、遗传差异、类质粒、嵌合基因、RNA编辑和核质互作等方面概述了植物线粒体遗传物质与CMS关系的研究进展。

Research progress of the relation between mitochondrial genetic material and CMS in plants is introduced in regarding to plant mitochondrial genome structure character , genetic diversity , plasmid-like , chimeric gene , RNA editing and nucleo-cytoplasmic interaction .

目的：构建含有HIV-1（中国株）gag-gp120嵌合基因的重组杆状病毒表达载体。

Objective : A new recombinant baculovirus transfer vector was constructed , in which a recombinant gene fragment encoding HIV-1 gag-gp120 chimeric gene was inserted .

目的构建含戊型肝炎病毒（HEV）开放读码框架（ORF2）和ORF3嵌合基因的重组质粒，并探讨其作为DNA疫苗的可能性。

Objective To construct a recombinant plasmid which contained fragments of HEV ORF2 and ORF3 chimeric gene , and then assess its feasibility for use as a DNA vaccine .

目的表达抗人肺癌单克隆抗体（mAb）LC1的人鼠嵌合基因工程抗体。

Aim To express human mouse chimeric genetic engineering antibody of monoclonal antibody ( mAb ) LC 1 to human pulmonary carcinoma .

将4个含有不同大小启动子区的克隆片段与GUS基因编码区连接构建成嵌合基因，通过基因枪轰击转入香蕉叶、根和果实的细胞后。

The 5 ′ - flanking fragments in different length were fused to the coding sequence of GUS gene . These constructs were delivered to leaf , root and fruit cells of banana via particle bombardment .

同时，也按照经典的Mariani系统构建了以barstar为核心的雄性不育恢复嵌合基因，并转化烟草获得kan抗性植株。

In the meantime , an expression system of chimeric male sterility restore gene with barstar had been constructed and Kan-resistant tobacco plants also regenerated .

普通烟草花叶病毒外壳蛋白基因已和花椰菜花叶病毒35S启动子及3′未端重组成嵌合基因。

The coat protein gene of tobacco mosaic virus strain OM have been combined with the 35S promoter and terminator of cauliflower mosaic virus as a chimeric gene .

CMS育性的恢复依赖核恢复基因，恢复基因可通过干扰CMS相关嵌合基因的活动，影响CMS相关嵌合基因转录本转录模式、转录本丰度以及转录本加工编辑和翻译等过程。

CMS fertility restoration is thought to rely on nuclear restorer gene , which can interfere with the activities of CMS-related chimeric gene and impact on transcript pattern , transcript abundance and transcript process of editing and translation of chimeric gene .

人Ⅰ型免疫缺陷病毒gag-env嵌合基因在重组痘苗中的表达

Expression of gag-env Chimeric Gene of Human Immunodeficiency Virus Type 1 in Recombinant Vaccinia Virus

结论：IL18和HIV1gaggp120嵌合基因的DNA疫苗联合免疫可诱导小鼠产生特异性细胞和体液免疫，且IL18发挥了免疫佐剂的作用。

CONCLUSION : The specific cellular and humoral immunity in mice can be induced by co-inoculating HIV-1 gag-gp120 chimeric gene DNA vaccine and IL-18 plasmid indicating that IL-18 augments immune response as immunoadjuvant .

转染BCR-ABL嵌合基因两种不同的方法，通过检测荧光素酶报告基因的方法，观察用两种不同的方法处理细胞后对EDAG基因表达的影响。

K562 cells were induced by PMA or transfected with BCR-ABL chimeric gene , and the expression of EDAG gene in the reporter cells was determined with luciferase assay .

关于CMS败育机理的研究有很多报道，线粒体基因组分子内或分子间频繁重组所形成的异常嵌合基因被认为是CMS产生的分子基础。

People have engaged in studying the mechanism of CMS Abortion for a long time , it is widely considered that abnormal chimeric gene in mitochondrial genome formed by intramolecular or intermolecular frequent recombination is the molecular basis and primary factor of CMS .

目的利用塞姆利基森林病毒（SFV）复制子构建新型真核表达载体，并对含HIV-1中国流行株B亚型核心蛋白p24及多表位MEG嵌合基因的核酸疫苗进行表达与鉴定。

Objective To construct a novel eukaryotic expression vector based on Semliki forest virus ( SFV ) replicon and express the DNA vaccine containing subtype core protein p24 and multi-epitope MEG chimeric gene of epidemic strain B of HIV-1 in China .

由于RGD水蛭素嵌合基因上游连接了金黄色葡萄球菌蛋白A（SPA）的信号肽序列，在IPTG诱导下两种嵌合分子都获得了分泌表达，表达产物主要集中在细胞周质空间。

Coli strain BL21 ( DE3 ), and gene expression was induced by IPTG . The target proteins were directed into the periplasmic space by the staphylococcal protein A ( SPA ) signal sequence preceding the RGD hirudin gene .

为研制灵敏、特异的抗人类T淋巴细胞白血病病毒（HTLV）抗体诊断试剂，将一段HTLVⅠ和HTLVⅡenv区嵌合基因在大肠杆菌中表达，获得的重组抗原具有良好活性。

For development of a sensitive and specific diagnostic kit of the human T-lymphotropic virus ( HTLV ), a chimeric envelope gene of the HTLV - ⅰ and HTLV - ⅱ was expressed in E. coli .